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Salsolinol improves angiotensin II‑induced myocardial fibrosis in vitro via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway

  • Authors:
    • Xian Zhang
    • Ze Shao
    • Yuchao Ni
    • Feilong Chen
    • Xia Yu
    • Jiasheng Wen
  • View Affiliations / Copyright

    Affiliations: Cardiology Department, Kunshan Hospital of Integrated Traditional Chinese and Western Medicine, Kunshan, Jiangsu 215332, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 527
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    Published online on: September 27, 2023
       https://doi.org/10.3892/etm.2023.12226
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Abstract

The clinical incidence of congestive heart failure (CHF) is very high and it poses a significant threat to the health of patients. The traditional Chinese medicine monomer salsolinol is widely used to treat similar symptoms of CHF. However, there have been no reports on the effect of salsolinol for the management of CHF and its effects on myocardial fibrosis. In the present study, salsolinol was used to treat angiotensin II (AngII)‑induced human cardiac fibroblasts (HCFs) and cell proliferation and migration were assessed using a CCK‑8, EdU staining assay and wound healing assay. Subsequently, immunofluorescence, western blotting and other techniques were used to detect indicators associated with cell fibrosis and relevant kits were used to detect markers of cellular inflammation and reactive oxygen species (ROS) production. Molecular docking analysis was used to predict the relationship between salsolinol and lysine‑specific histone demethylase 1A (LSD1). Subsequently, the expression of LSD1 in the serum of CHF patients was detected by reverse transcription‑quantitative PCR. Finally, LSD1 was overexpressed in cells to explore the regulatory mechanism of salsolinol in AngII‑induced HFCs. Salsolinol reduced the proliferation and migration. Salsolinol reduced the expression of fibrosis marker proteins α‑smooth muscle actin, Collagen I and Collagen III in a concentration‑dependent manner, thereby reducing cell fibrosis. In addition, salsolinol reduced the levels of TNF‑α and IL‑6 in the cell supernatant and ROS production following AngII induction. Salsolinol inhibited LSD1 expression and regulated the STAT3/Notch‑1 signaling pathway. Upregulation of LSD1 reversed the effects of salsolinol on AngII‑induced HCFs. Salsolinol inhibited LSD1 via regulation of the STAT3/Notch‑1 signaling pathway to improve Ang II‑induced myocardial fibrosis in vitro.
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1 

Kennelly P, Sapkota R, Azhar M, Cheema FH, Conway C and Hameed A: Diuretic therapy in congestive heart failure. Acta Cardiol. 77:97–104. 2022.PubMed/NCBI View Article : Google Scholar

2 

Abdelbasset WK and Alqahtani BA: A randomized controlled trial on the impact of moderate-intensity continuous aerobic exercise on the depression status of middle-aged patients with congestive heart failure. Medicine (Baltimore). 98(e15344)2019.PubMed/NCBI View Article : Google Scholar

3 

Wen J, Li M, Zhang W, Wang H, Bai Y, Hao J, Liu C, Deng K and Zhao Y: Role of higenamine in heart diseases: A mini-review. Front Pharmacol. 12(798495)2022.PubMed/NCBI View Article : Google Scholar

4 

Liu M, Li Y, Tang Y, Zheng L and Peng C: Synergistic effect of aconiti lateralis radix praeparata water-soluble alkaloids and ginseng radix et rhizoma total ginsenosides compatibility on acute heart failure rats. J Chromatogr B Analyt Technol Biomed Life Sci. 1137(121935)2020.PubMed/NCBI View Article : Google Scholar

5 

Xu X, Xie X, Zhang H, Wang P, Li G, Chen J, Chen G, Cao X, Xiong L, Peng F and Peng C: Water-soluble alkaloids extracted from aconiti radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway. Biomed Pharmacother. 135(111184)2021.PubMed/NCBI View Article : Google Scholar

6 

Yang Y, Hu P, Zhou X, Wu P, Si X, Lu B, Zhu Y and Xia Y: Transcriptome analysis of aconitum carmichaelii and exploration of the salsolinol biosynthetic pathway. Fitoterapia. 140(104412)2020.PubMed/NCBI View Article : Google Scholar

7 

Wen J, Zhang L, Liu H, Wang J, Li J, Yang Y, Wang Y, Cai H, Li R and Zhao Y: Salsolinol attenuates doxorubicin-induced chronic heart failure in rats and improves mitochondrial function in H9c2 cardiomyocytes. Front Pharmacol. 10(1135)2019.PubMed/NCBI View Article : Google Scholar

8 

Zhang S, Liu M, Yao Y, Yu B and Liu H: Targeting LSD1 for acute myeloid leukemia (AML) treatment. Pharmacol Res. 164(105335)2021.PubMed/NCBI View Article : Google Scholar

9 

Guenantin AC, Jebeniani I, Leschik J, Watrin E, Bonne G, Vignier N and Pucéat M: Targeting the histone demethylase LSD1 prevents cardiomyopathy in a mouse model of laminopathy. J Clin Invest. 131(e136488)2021.PubMed/NCBI View Article : Google Scholar

10 

Huo JL, Jiao L, An Q, Chen X, Qi Y, Wei B, Zheng Y, Shi X, Gao E, Liu HM, et al: Myofibroblast deficiency of LSD1 alleviates TAC-induced heart failure. Circ Res. 129:400–413. 2021.PubMed/NCBI View Article : Google Scholar

11 

Bao J, Ye C, Zheng Z and Zhou Z: Fmr1 protects cardiomyocytes against lipopolysaccharide-induced myocardial injury. Exp Ther Med. 16:1825–1833. 2018.PubMed/NCBI View Article : Google Scholar

12 

Evans DA: History of the harvard ChemDraw project. Angew Chem Int Ed Engl. 53:11140–11145. 2014.PubMed/NCBI View Article : Google Scholar

13 

Lin Z, Zhang Z, Ye X, Zhu M, Li Z, Chen Y and Huang S: Based on network pharmacology and molecular docking to predict the mechanism of Huangqi in the treatment of castration-resistant prostate cancer. PLoS One. 17(e0263291)2022.PubMed/NCBI View Article : Google Scholar

14 

Zhang Y, Liu X, Long J, Cheng X, Wang X and Feng X: Exploring active compounds and mechanisms of angong niuhuang wan on ischemic stroke based on network pharmacology and molecular docking. Evid Based Complement Alternat Med. 2022(2443615)2022.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Yu H, Basu S and Hallow KM: Cardiac and renal function interactions in heart failure with reduced ejection fraction: A mathematical modeling analysis. PLoS Comput Biol. 16(e1008074)2020.PubMed/NCBI View Article : Google Scholar

17 

González A, Schelbert EB, Diez J and Butler J: Myocardial interstitial fibrosis in heart failure: Biological and translational perspectives. J Am Coll Cardiol. 71:1696–1706. 2018.PubMed/NCBI View Article : Google Scholar

18 

Rao M, Wang X, Guo G, Wang L, Chen S, Yin P, Chen K, Chen L, Zhang Z, Chen X, et al: Resolving the intertwining of inflammation and fibrosis in human heart failure at single-cell level. Basic Res Cardiol. 116(55)2021.PubMed/NCBI View Article : Google Scholar

19 

Kurose H: Cardiac fibrosis and fibroblasts. Cells. 10(1716)2021.PubMed/NCBI View Article : Google Scholar

20 

Tallquist MD: Cardiac fibroblast diversity. Annu Rev Physiol. 82:63–78. 2020.PubMed/NCBI View Article : Google Scholar

21 

Liu M, López de Juan Abad B and Cheng K: Cardiac fibrosis: Myofibroblast-mediated pathological regulation and drug delivery strategies. Adv Drug Deliv Rev. 173:504–519. 2021.PubMed/NCBI View Article : Google Scholar

22 

Maruyama K and Imanaka-Yoshida K: The pathogenesis of cardiac fibrosis: A review of recent progress. Int J Mol Sci. 23(2617)2022.PubMed/NCBI View Article : Google Scholar

23 

Hong LL, Zhao Y, Chen WD, Yang CY, Li GZ, Wang HS and Cheng XY: Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats. Front Cardiovasc Med. 9(913661)2022.PubMed/NCBI View Article : Google Scholar

24 

Guo S, Li P, Fu B, Chuo W, Gao K, Zhang W, Wang J, Chen J and Wang W: Systems-biology dissection of mechanisms and chemical basis of herbal formula in treating chronic myocardial ischemia. Pharmacol Res. 114:196–208. 2016.PubMed/NCBI View Article : Google Scholar

25 

Zhang X, Li LX, Yu C, Nath KA, Zhuang S and Li X: Targeting lysine-specific demethylase 1A inhibits renal epithelial-mesenchymal transition and attenuates renal fibrosis. FASEB J. 36(e22122)2022.PubMed/NCBI View Article : Google Scholar

26 

Pan X, Li J, Tu X, Wu C, Liu H, Luo Y, Dong X, Li X, Pan LL and Sun J: Lysine-specific demethylase-1 regulates fibroblast activation in pulmonary fibrosis via TGF-β1/Smad3 pathway. Pharmacol Res. 152(104592)2020.PubMed/NCBI View Article : Google Scholar

27 

Zhang H, Xing J and Zhao L: Lysine-specific demethylase 1 induced epithelial-mesenchymal transition and promoted renal fibrosis through Jagged-1/Notch signaling pathway. Hum Exp Toxicol. 40 (12 Suppl):S203–S214. 2021.PubMed/NCBI View Article : Google Scholar

28 

Chen X, Su J, Feng J, Cheng L, Li Q, Qiu C and Zheng Q: TRIM72 contributes to cardiac fibrosis via regulating STAT3/Notch-1 signaling. J Cell Physiol. 234:17749–17756. 2019.PubMed/NCBI View Article : Google Scholar

29 

Zhang X, Zheng C, Gao Z, Wang L, Chen C, Zheng Y and Meng Y: PKM2 promotes angiotensin-II-induced cardiac remodelling by activating TGF-β/Smad2/3 and Jak2/Stat3 pathways through oxidative stress. J Cell Mol Med. 25:10711–10723. 2021.PubMed/NCBI View Article : Google Scholar

30 

Skoumal R, Tóth M, Serpi R, Rysä J, Leskinen H, Ulvila J, Saiho T, Aro J, Ruskoaho H, Szokodi I and Kerkelä R: Parthenolide inhibits STAT3 signaling and attenuates angiotensin II-induced left ventricular hypertrophy via modulation of fibroblast activity. J Mol Cell Cardiol. 50:634–641. 2011.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Shao Z, Ni Y, Chen F, Yu X and Wen J: Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway. Exp Ther Med 26: 527, 2023.
APA
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., & Wen, J. (2023). Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway. Experimental and Therapeutic Medicine, 26, 527. https://doi.org/10.3892/etm.2023.12226
MLA
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., Wen, J."Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway". Experimental and Therapeutic Medicine 26.5 (2023): 527.
Chicago
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., Wen, J."Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway". Experimental and Therapeutic Medicine 26, no. 5 (2023): 527. https://doi.org/10.3892/etm.2023.12226
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Shao Z, Ni Y, Chen F, Yu X and Wen J: Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway. Exp Ther Med 26: 527, 2023.
APA
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., & Wen, J. (2023). Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway. Experimental and Therapeutic Medicine, 26, 527. https://doi.org/10.3892/etm.2023.12226
MLA
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., Wen, J."Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway". Experimental and Therapeutic Medicine 26.5 (2023): 527.
Chicago
Zhang, X., Shao, Z., Ni, Y., Chen, F., Yu, X., Wen, J."Salsolinol improves angiotensin II‑induced myocardial fibrosis <em>in vitro</em> via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway". Experimental and Therapeutic Medicine 26, no. 5 (2023): 527. https://doi.org/10.3892/etm.2023.12226
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