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Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats

  • Authors:
    • Yongli Wang
    • Xingcun Li
    • Hongliang Gao
    • Qian Lu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Huzhou Central Hospital, Huzhou Basic and Clinical Translation of Orthopedics Key Laboratory, Huzhou, Zhejiang 313300, P.R. China, Public Health Section, Huzhou Central Hospital, Huzhou Basic and Clinical Translation of Orthopedics Key Laboratory, Huzhou, Zhejiang 313300, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 538
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    Published online on: October 2, 2023
       https://doi.org/10.3892/etm.2023.12237
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Abstract

Osteoporosis is a systemic bone metabolic disorder that plagues the health and quality of life of the elderly. Autophagy plays an important role in bone formation while maintaining the homeostasis of the body. Trehalose is a mTOR‑independent autophagy inducer, but to the best of our knowledge, there is no rat model of postmenopausal osteoporosis. The present study found that trehalose can delay postmenopausal osteoporosis in rats, which may be achieved by inducing and enhancing AKT/transcription factor EB pathway‑dependent autophagy flow. The specific mechanism of its occurrence needs to be further studied. Trehalose‑containing drugs are promising for delaying postmenopausal osteoporosis. Hematoxylin and eosin (H&E) staining, western blotting, micro computerized tomography (CT) scanning and Transmission electron microscopy were used to investigate the role of trehalose in postmenopausal osteoporosis rat model at protein, cell and histology aspects. According to the H&E staining results, the bone trabecular histological structure of the trehalose group was superior to that of the model group. The Micro CT scanning indicated the imaging structure of bone trabeculae in the trehalose group was superior to than that in the model group. Western blotting indicated the activation of autophagic flow in trehalose group, the autophagy degree of the trehalose group is greater than that of the model group; Transmission electron microscopy indicated the autophagy degree of the Trehalose group was greater than that of the model group under electron microscopy. Trehalose can delay postmenopausal osteoporosis in rats, which may be achieved by inducing and enhancing Akt/TFEB pathway‑dependent autophagy flow.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Li X, Gao H and Lu Q: Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats. Exp Ther Med 26: 538, 2023.
APA
Wang, Y., Li, X., Gao, H., & Lu, Q. (2023). Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats. Experimental and Therapeutic Medicine, 26, 538. https://doi.org/10.3892/etm.2023.12237
MLA
Wang, Y., Li, X., Gao, H., Lu, Q."Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats". Experimental and Therapeutic Medicine 26.5 (2023): 538.
Chicago
Wang, Y., Li, X., Gao, H., Lu, Q."Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats". Experimental and Therapeutic Medicine 26, no. 5 (2023): 538. https://doi.org/10.3892/etm.2023.12237
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Li X, Gao H and Lu Q: Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats. Exp Ther Med 26: 538, 2023.
APA
Wang, Y., Li, X., Gao, H., & Lu, Q. (2023). Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats. Experimental and Therapeutic Medicine, 26, 538. https://doi.org/10.3892/etm.2023.12237
MLA
Wang, Y., Li, X., Gao, H., Lu, Q."Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats". Experimental and Therapeutic Medicine 26.5 (2023): 538.
Chicago
Wang, Y., Li, X., Gao, H., Lu, Q."Trehalose delays postmenopausal osteoporosis by enhancing AKT/TFEB pathway‑dependent autophagy flow in rats". Experimental and Therapeutic Medicine 26, no. 5 (2023): 538. https://doi.org/10.3892/etm.2023.12237
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