Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-2024 Volume 27 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2024 Volume 27 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer

  • Authors:
    • Ying Wang
    • Botao Yu
    • Mengyuan Qu
    • Fengjuan Liu
    • Xiao Wu
  • View Affiliations / Copyright

    Affiliations: Department of Nosocomial Infection, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong 266042, P.R. China, Department of Emergency Medicine, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong 266042, P.R. China, Department of Radiophysics, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong 266042, P.R. China, Ward for Phase I Clinical Trial, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong 266042, P.R. China, Department of Pulmonary and Critical Care Medicine, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong 266042, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 109
    |
    Published online on: January 22, 2024
       https://doi.org/10.3892/etm.2024.12397
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Lung cancer is a harmful type of malignancy and the leading cause of cancer‑associated mortality. It is therefore imperative to develop novel drugs effective for treating this cancer. The Traditional Chinese Medicine compound Britannin has been previously reported to inhibit the development of certain cancers, such as pancreatic, breast and liver cancer. Moreover, Kruppel‑like factor 5 (KLF5) has been identified an on oncogene in lung cancer. In the present study, the possible regulatory effects and underlying mechanism of Britannin in lung cancer were investigated. A549 and 16HBE cells were treated with different concentrations of Britannin. Subsequently, Cell counting kit‑8, EdU staining and colony formation assays were used to detect the proliferative ability of these cells. Cell migration was detected by wound healing and Transwell assays, respectively. XF96 extracellular flux analyzer was used to analyze the extent of extracellular acidification and oxygen consumption rate in cells, whereas assay kits were used to detect glucose and lactic acid levels in the cell supernatant. The targeting effect between Britannin and the KLF5 protein was investigated using molecular docking technology. The protein expression levels of KLF5 in cells challenged with Britannin was detected by western blotting. Finally, overexpression of KLF5 in A549 cells was performed before cell proliferation, migration and the glycolysis rate were measured to explore the regulatory effects of Britannin. Britannin was found to inhibit the proliferation, migration and glycolysis of lung cancer cells, during which the protein expression levels of KLF5 were decreased. This suggests that Britannin regulated the expression of KLF5 in A549 cells. Overexpression of KLF5 reversed the inhibitory effects of Britannin on the proliferation, migration and glycolysis in lung cancer cells. In conclusion, these results suggest that Britannin can inhibit cell proliferation, migration and glycolysis by downregulating KLF5 expression in lung cancer cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Greenberg AK, Yee H and Rom WN: Preneoplastic lesions of the lung. Respir Res. 3(20)2002.PubMed/NCBI View Article : Google Scholar

2 

Mao Y, Yang D, He J and Krasna MJ: Epidemiology of Lung Cancer. Surg Oncol Clin N Am. 25:439–445. 2016.PubMed/NCBI View Article : Google Scholar

3 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 71:209–249. 2021.PubMed/NCBI View Article : Google Scholar

4 

Lemjabbar-Alaoui H, Hassan OU, Yang YW and Buchanan P: Lung cancer: Biology and treatment options. Biochim Biophys Acta. 1856:189–210. 2015.PubMed/NCBI View Article : Google Scholar

5 

Bilfinger T, Keresztes R, Albano D and Nemesure B: Five-Year Survival Among Stage IIIA Lung Cancer Patients Receiving Two Different Treatment Modalities. Med Sci Monit. 22:2589–2594. 2016.PubMed/NCBI View Article : Google Scholar

6 

Johnson ML and Patel JD: Chemotherapy and targeted therapeutics as maintenance of response in advanced non-small cell lung cancer. Semin Oncol. 41:93–100. 2014.PubMed/NCBI View Article : Google Scholar

7 

Hui H, Wang M, Guo H, Wang Y and Shi B: Poziotinib-induced cutaneous adverse reactions in the treatment of non-small cell lung cancer: A case report. Int J Dermatol. 61:769–770. 2022.PubMed/NCBI View Article : Google Scholar

8 

Leonardi GC, Oxnard GR, Haas A, Lang JP, Williams JS and Awad MM: Diabetic Ketoacidosis as an Immune-related Adverse Event from Pembrolizumab in Non-Small Cell Lung Cancer. J Immunother. 40:249–251. 2017.PubMed/NCBI View Article : Google Scholar

9 

Ganapathy-Kanniappan S and Geschwind JF: Tumor glycolysis as a target for cancer therapy: progress and prospects. Mol Cancer. 12(152)2013.PubMed/NCBI View Article : Google Scholar

10 

Zhang L, Zhang Z and Yu Z: Identification of a novel glycolysis-related gene signature for predicting metastasis and survival in patients with lung adenocarcinoma. J Transl Med. 17(423)2019.PubMed/NCBI View Article : Google Scholar

11 

Smolle E, Leko P, Stacher-Priehse E, Brcic L, El-Heliebi A, Hofmann L, Quehenberger F, Hrzenjak A, Popper HH, Olschewski H and Leithner K: Distribution and prognostic significance of gluconeogenesis and glycolysis in lung cancer. Mol Oncol. 14:2853–2867. 2020.PubMed/NCBI View Article : Google Scholar

12 

Li X, Yang G, Li X, Zhang Y, Yang J, Chang J, Sun X, Zhou X, Guo Y, Xu Y, Liu J and Bensoussan A: Traditional Chinese medicine in cancer care: a review of controlled clinical studies published in chinese. PLoS One. 8(e60338)2013.PubMed/NCBI View Article : Google Scholar

13 

Su XL, Wang JW, Che H, Wang CF, Jiang H, Lei X, Zhao W, Kuang HX and Wang QH: Clinical application and mechanism of traditional Chinese medicine in treatment of lung cancer. Chin Med J (Engl). 133(24):2987–2997. 2020.PubMed/NCBI View Article : Google Scholar

14 

Wei Z, Chen J, Zuo F, Guo J, Sun X, Liu D and Liu C: Traditional Chinese Medicine has great potential as candidate drugs for lung cancer: A review. J Ethnopharmacol. 300(115748)2023.PubMed/NCBI View Article : Google Scholar

15 

Mohammadlou H, Hamzeloo-Moghadam M, Yami A, Feizi F, Moeinifard M and Gharehbaghian A: Britannin a Sesquiterpene Lactone from Inula aucheriana Exerted an Anti-leukemic Effect in Acute Lymphoblastic Leukemia (ALL) Cells and Enhanced the Sensitivity of the Cells to Vincristine. Nutr Cancer. 74:965–977. 2022.PubMed/NCBI View Article : Google Scholar

16 

Lu H, Wu Z, Wang Y, Zhao D, Zhang B and Hong M: Study on inhibition of Britannin on triple-negative breast carcinoma through degrading ZEB1 proteins. Phytomedicine. 104(154291)2022.PubMed/NCBI View Article : Google Scholar

17 

Luo Y and Chen C: The roles and regulation of the KLF5 transcription factor in cancers. Cancer Sci. 112:2097–2117. 2021.PubMed/NCBI View Article : Google Scholar

18 

Zhang H, Shao F, Guo W, Gao Y and He J: Knockdown of KLF5 promotes cisplatin-induced cell apoptosis via regulating DNA damage checkpoint proteins in non-small cell lung cancer. Thorac Cancer. 10:1069–1077. 2019.PubMed/NCBI View Article : Google Scholar

19 

Tung CH, Huang MF, Liang CH, Wu YY, Wu JE, Hsu CL, Chen YL and Hong TM: alpha-Catulin promotes cancer stemness by antagonizing WWP1-mediated KLF5 degradation in lung cancer. Theranostics. 12:1173–1186. 2022.PubMed/NCBI View Article : Google Scholar

20 

Lu X, Zhao N, Duan G, Deng Z and Lu Y: Testis developmental related gene 1 promotes non-small-cell lung cancer through the microRNA-214-5p/Kruppel-like factor 5 axis. Bioengineered. 13:603–616. 2022.PubMed/NCBI View Article : Google Scholar

21 

Shi J, Yang C, An J, Hao D, Liu C, Liu J, Sun J and Jiang J: KLF5-induced BBOX1-AS1 contributes to cell malignant phenotypes in non-small cell lung cancer via sponging miR-27a-5p to up-regulate MELK and activate FAK signaling pathway. J Exp Clin Cancer Res. 40(148)2021.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Zhang YF, Zhang ZH, Li MY, Wang JY, Xing Y, Ri M, Jin CH, Xu GH, Piao LX, Zuo HX, Jin HL, Ma J and Jin X: Britannin stabilizes T cell activity and inhibits proliferation and angiogenesis by targeting PD-L1 via abrogation of the crosstalk between Myc and HIF-1alpha in cancer. Phytomedicine. 81(153425)2021.PubMed/NCBI View Article : Google Scholar

24 

Li K, Zhou Y, Chen Y, Zhou L and Liang J: A novel natural product, britanin, inhibits tumor growth of pancreatic cancer by suppressing nuclear factor-kappaB activation. Cancer Chemother Pharmacol. 85:699–709. 2020.PubMed/NCBI View Article : Google Scholar

25 

Cui YQ, Liu YJ and Zhang F: The suppressive effects of Britannin (Bri) on human liver cancer through inducing apoptosis and autophagy via AMPK activation regulated by ROS. Biochem Biophys Res Commun. 497:916–923. 2018.PubMed/NCBI View Article : Google Scholar

26 

Karlstaedt A, Barrett M, Hu R, Gammons ST and Ky B: Cardio-Oncology: Understanding the Intersections Between Cardiac Metabolism and Cancer Biology. JACC Basic Transl Sci. 6:705–718. 2021.PubMed/NCBI View Article : Google Scholar

27 

Danhier P, Banski P, Payen VL, Grasso D, Ippolito L, Sonveaux P and Porporato PE: Cancer metabolism in space and time: Beyond the Warburg effect. Biochim Biophys Acta Bioenerg. 1858:556–572. 2017.PubMed/NCBI View Article : Google Scholar

28 

Hong H, Luo B, Xie Z, Li M, Xu Q, He Z and Peng Z: Retracted: Britannin mediates apoptosis and glycolysis of T-cell lymphoblastic lymphoma cells by AMPK-dependent autophagy. J Biochem Mol Toxicol. e23211:2022.PubMed/NCBI View Article : Google Scholar

29 

Gong T, Cui L, Wang H, Wang H and Han N: Knockdown of KLF5 suppresses hypoxia-induced resistance to cisplatin in NSCLC cells by regulating HIF-1α-dependent glycolysis through inactivation of the PI3K/Akt/mTOR pathway. J Transl Med. 16(164)2018.PubMed/NCBI View Article : Google Scholar

30 

Xia W, Bai H, Deng Y and Yang Y: PLA2G16 is a mutant p53/KLF5 transcriptional target and promotes glycolysis of pancreatic cancer. J Cell Mol Med. 24:12642–12655. 2020.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Yu B, Qu M, Liu F and Wu X: Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer. Exp Ther Med 27: 109, 2024.
APA
Wang, Y., Yu, B., Qu, M., Liu, F., & Wu, X. (2024). Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer. Experimental and Therapeutic Medicine, 27, 109. https://doi.org/10.3892/etm.2024.12397
MLA
Wang, Y., Yu, B., Qu, M., Liu, F., Wu, X."Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer". Experimental and Therapeutic Medicine 27.3 (2024): 109.
Chicago
Wang, Y., Yu, B., Qu, M., Liu, F., Wu, X."Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer". Experimental and Therapeutic Medicine 27, no. 3 (2024): 109. https://doi.org/10.3892/etm.2024.12397
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yu B, Qu M, Liu F and Wu X: Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer. Exp Ther Med 27: 109, 2024.
APA
Wang, Y., Yu, B., Qu, M., Liu, F., & Wu, X. (2024). Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer. Experimental and Therapeutic Medicine, 27, 109. https://doi.org/10.3892/etm.2024.12397
MLA
Wang, Y., Yu, B., Qu, M., Liu, F., Wu, X."Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer". Experimental and Therapeutic Medicine 27.3 (2024): 109.
Chicago
Wang, Y., Yu, B., Qu, M., Liu, F., Wu, X."Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer". Experimental and Therapeutic Medicine 27, no. 3 (2024): 109. https://doi.org/10.3892/etm.2024.12397
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team