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Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance

  • Authors:
    • Yan Zhang
    • Xiaodong Zhang
    • Aiping Yu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China, Department of Ultrasound, Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 116
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    Published online on: January 29, 2024
       https://doi.org/10.3892/etm.2024.12403
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Abstract

The expression of macrophage activation‑specific factors in hyperplastic scar (HS) tissues during hyperplasia phase was detected by antibody array imprinted membrane method and the role of macrophage activation in the natural evolution of HS was explored. A total of 83 patients with HS admitted to the Affiliated Hospital of Beihua University (Jilin, China) between February 2021 and July 2021 were enrolled. The clinical data of the patients were retrospectively analyzed. These patients were divided into the hyperplasia HS group (n=26) and the decline HS group (the HS tissues ceased to grow and were in regression periods; n=57) according to the time of scar formation and clinical characteristics. The HS tissues were collected from patients in both groups. The contents of IL‑12, IL‑10, VEGF and basic fibroblast growth factor (bFGF) were detected by antibody array imprinted membrane method and the contents of IL‑12, IL‑10, VEGF and bFGF in tissues with various groups of tissues and clinical features were compared. The connection between macrophage activation‑specific factors with VEGF and bFGF was analyzed using Pearson correlation analysis. The contents of IL‑10 (9.48±1.06), VEGF (24.15±2.64) and bFGF (37.48±2.56) were much lower and IL‑12 levels (16.45±0.85) were strongly higher in hyperplasia HS group compared with those in the decline HS group (14.56±1.26 for IL‑10, 27.85±2.63 for VEGF, 43.15±3.16 for bFGF and 10.46±0.75 for IL‑12, P<0.001). In the hyperplasia HS group, the contents of IL‑10, VEGF and bFGF were obviously higher and the IL‑12 levels were markedly lower in patients with age ≥30 years, protuberance height <2 mm, soft flexibility, low hyperemia degree and no concomitant symptoms than those in the patients with age <30 years, protuberance height ≥2 mm, hard flexibility, high hyperemia degree and concomitant symptoms (P<0.001). Pearson correlation analysis showed that IL‑12 was negatively correlated with VEGF and bFGF (r=‑0.328, 0.600, P<0.01). IL‑10 was positively correlated with VEGF and bFGF (r=0.486, 0.684, respectively, P<0.001). In conclusion, macrophage activation‑specific factors were abnormally expressed in hyperplasia HS, mainly M1 macrophages, accompanied by severe inflammatory reaction. The transformation of M1 macrophage into M2 macrophage usually occurred during the declining HS phase, which accelerated scar formation by promoting the formation of fibroblasts and angiogenesis. Detection of macrophage activation‑specific factors may contribute to evaluate the clinical stage of HS.
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1 

Ogawa R, Dohi T, Tosa M, Aoki M and Akaishi S: The latest strategy for keloid and hypertrophic scar prevention and treatment: The Nippon medical school (NMS) Protocol. J Nippon Med Sch. 88:2–9. 2021.PubMed/NCBI View Article : Google Scholar

2 

Bi M, Sun P, Li D, Dong Z and Chen Z: Intralesional injection of botulinum toxin type A compared with intralesional injection of corticosteroid for the treatment of hypertrophic scar and keloid: A systematic review and meta-analysis. Med Sci Monit. 25:2950–2958. 2019.PubMed/NCBI View Article : Google Scholar

3 

Nischwitz SP, Fink J, Schellnegger M, Luze H, Bubalo V, Tetyczka C, Roblegg E, Holecek C, Zacharias M, Kamolz LP and Kotzbeck P: The role of local inflammation and hypoxia in the formation of hypertrophic scars-a new model in the Duroc pig. Int J Mol Sci. 24(316)2022.PubMed/NCBI View Article : Google Scholar

4 

Paish HL, Kalson NS, Smith GR, Del Carpio Pons A, Baldock TE, Smith N, Swist-Szulik K, Weir DJ, Bardgett M, Deehan DJ, et al: Fibroblasts promote inflammation and pain via IL-1α induction of the monocyte chemoattractant chemokine (C-C Motif) ligand 2. Am J Pathol. 188:696–714. 2018.PubMed/NCBI View Article : Google Scholar

5 

Hedayatyanfard K, Haddadi NS, Ziai SA, Karim H, Niazi F, Steckelings UM, Habibi B, Modarressi A and Dehpour AR: The renin-angiotensin system in cutaneous hypertrophic scar and keloid formation. Exp Dermatol. 29:902–909. 2020.PubMed/NCBI View Article : Google Scholar

6 

Li J, Wang J, Wang Z, Xia Y, Zhou M, Zhong A and Sun J: Experimental models for cutaneous hypertrophic scar research. Wound Repair Regen. 28:126–144. 2020.PubMed/NCBI View Article : Google Scholar

7 

Bahrami M, Haji Molla Hoseini M, Rezaei M and Ziai SA: Umbelliprenin increases the M1/M2 ratio of macrophage polarization and improves the M1 macrophage activity in THP-1 cells cocultured with AGS cells. Evid Based Complement Alternat Med. 2021(9927747)2021.PubMed/NCBI View Article : Google Scholar

8 

Wang HT, Han JT and Hu DH: Research advances on the role and mechanism of inflammatory response in the formation of hypertrophic scars and keloids. Zhonghua Shao Shang Za Zhi. 37:490–494. 2021.PubMed/NCBI View Article : Google Scholar : (In Chinese).

9 

Weber KJ, Sauer M, He L, Tycksen E, Kalugotla G, Razani B and Schilling JD: PPARγ deficiency suppresses the release of IL-1β and IL-1α in macrophages via a type 1 IFN-dependent mechanism. J Immunol. 201:2054–2069. 2018.PubMed/NCBI View Article : Google Scholar

10 

Barone N, Safran T, Vorstenbosch J, Davison PG, Cugno S and Murphy AM: Current advances in hypertrophic scar and keloid management. Semin Plast Surg. 35:145–152. 2021.PubMed/NCBI View Article : Google Scholar

11 

Lei JA, Zhou Y and Qin ZL: Research advances on inflammatory responses involved in keloid development. Zhonghua Shao Shang Za Zhi. 37:591–595. 2021.PubMed/NCBI View Article : Google Scholar : (In Chinese).

12 

Ogawa R: The most current algorithms for the treatment and prevention of hypertrophic scars and keloids: A 2020 update of the algorithms published 10 years ago. Plast Reconstr Surg. 149:79e–94e. 2022.PubMed/NCBI View Article : Google Scholar

13 

Bojanic C, To K, Hatoum A, Shea J, Seah KTM, Khan W and Malata CM: Mesenchymal stem cell therapy in hypertrophic and keloid scars. Cell Tissue Res. 383:915–930. 2021.PubMed/NCBI View Article : Google Scholar

14 

Lee HJ and Jang YJ: Recent understandings of biology, prophylaxis and treatment strategies for hypertrophic scars and keloids. Int J Mol Sci. 19(711)2018.PubMed/NCBI View Article : Google Scholar

15 

Frech FS, Hernandez L, Urbonas R, Zaken GA, Dreyfuss I and Nouri K: Hypertrophic scars and keloids: Advances in treatment and review of established therapies. Am J Clin Dermatol. 24:225–245. 2023.PubMed/NCBI View Article : Google Scholar

16 

Knowles A and Glass DA II: Keloids and hypertrophic scars. Dermatol Clin. 41:509–517. 2023.PubMed/NCBI View Article : Google Scholar

17 

Le X and Wu WW: The therapeutic effect of Interleukin-18 on hypertrophic scar through inducing Fas ligand expression. Burns. 47:430–438. 2021.PubMed/NCBI View Article : Google Scholar

18 

Wei J, Wang Z, Zhong C, Ding H, Wang X and Lu S: LncRNA MIR503HG promotes hypertrophic scar progression via miR-143-3p-mediated Smad3 expression. Wound Repair Regen. 29:792–800. 2021.PubMed/NCBI View Article : Google Scholar

19 

Zhang D, Li B and Zhao M: Therapeutic strategies by regulating interleukin family to suppress inflammation in hypertrophic scar and keloid. Front Pharmacol. 12(667763)2021.PubMed/NCBI View Article : Google Scholar

20 

Shi J, Shi S, Xie W, Zhao M, Li Y, Zhang J, Li N, Bai X, Cai W, Hu X, et al: IL-10 alleviates lipopolysaccharide-induced skin scarring via IL-10R/STAT3 axis regulating TLR4/NF-κB pathway in dermal fibroblasts. J Cell Mol Med. 25:1554–1567. 2021.PubMed/NCBI View Article : Google Scholar

21 

Chen J, Zhou R, Liang Y, Fu X, Wang D and Wang C: Blockade of lncRNA-ASLNCS5088-enriched exosome generation in M2 macrophages by GW4869 dampens the effect of M2 macrophages on orchestrating fibroblast activation. FASEB J. 33:12200–12212. 2019.PubMed/NCBI View Article : Google Scholar

22 

Xu X, Gu S, Huang X, Ren J, Gu Y, Wei C, Lian X, Li H, Gao Y, Jin R, et al: The role of macrophages in the formation of hypertrophic scars and keloids. Burns Trauma. 8(tkaa006)2020.PubMed/NCBI View Article : Google Scholar

23 

Li T, Chen W, Zhang Q and Deng C: Human-specific gene CHRFAM7A mediates M2 macrophage polarization via the Notch pathway to ameliorate hypertrophic scar formation. Biomed Pharmacother. 131(110611)2020.PubMed/NCBI View Article : Google Scholar

24 

Wilgus TA: Vascular endothelial growth factor and cutaneous scarring. Adv Wound Care (New Rochelle). 8:671–678. 2019.PubMed/NCBI View Article : Google Scholar

25 

Apte RS, Chen DS and Ferrara N: VEGF in signaling and disease: Beyond discovery and development. Cell. 176:1248–1264. 2019.PubMed/NCBI View Article : Google Scholar

26 

Qi X, Liu Y and Yang M: Circ_0057452 functions as a ceRNA in hypertrophic scar fibroblast proliferation and VEGF expression by regulating TGF-β2 expression and adsorbing miR-145-5p. Am J Transl Res. 13:6200–6210. 2021.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhang X and Yu A: Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance. Exp Ther Med 27: 116, 2024.
APA
Zhang, Y., Zhang, X., & Yu, A. (2024). Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance. Experimental and Therapeutic Medicine, 27, 116. https://doi.org/10.3892/etm.2024.12403
MLA
Zhang, Y., Zhang, X., Yu, A."Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance". Experimental and Therapeutic Medicine 27.3 (2024): 116.
Chicago
Zhang, Y., Zhang, X., Yu, A."Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance". Experimental and Therapeutic Medicine 27, no. 3 (2024): 116. https://doi.org/10.3892/etm.2024.12403
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhang X and Yu A: Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance. Exp Ther Med 27: 116, 2024.
APA
Zhang, Y., Zhang, X., & Yu, A. (2024). Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance. Experimental and Therapeutic Medicine, 27, 116. https://doi.org/10.3892/etm.2024.12403
MLA
Zhang, Y., Zhang, X., Yu, A."Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance". Experimental and Therapeutic Medicine 27.3 (2024): 116.
Chicago
Zhang, Y., Zhang, X., Yu, A."Expression of macrophage activation‑specific factors in hyperplastic scar tissue during hyperplasia phase by antibody array blotting membrane assay and its clinical significance". Experimental and Therapeutic Medicine 27, no. 3 (2024): 116. https://doi.org/10.3892/etm.2024.12403
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