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GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway

  • Authors:
    • Ye Ma
    • Lixing Zhang
    • Xin Gao
    • Dongming Zhu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Medical Laboratory, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 118
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    Published online on: January 29, 2024
       https://doi.org/10.3892/etm.2024.12407
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Abstract

Pancreatic cancer (PC) is a deadly and aggressive disease, which is characterized by poor prognosis. It has been reported that glutathione peroxidase 3 (GPX3) is involved in the development of several types of cancer. The present study aimed to explore the regulatory role of GPX3 in PC and uncover its underlying mechanism. Bioinformatics analysis was initially carried out to predict the expression profile of GPX3 in PC and its association with prognosis. The expression levels of GPX3 were also detected in PC cells by reverse transcription‑quantitative PCR and western blot analysis. Following transfection to induce GPX3 overexpression, the proliferation ability of PC cells was assessed by Cell Counting Kit‑8, colony formation and 5‑ethynyl‑2'‑deoxyuridine incorporation assays. In addition, wound healing and Transwell assays were performed to evaluate the migration and invasion abilities of PC cells. Cell apoptosis was assessed by flow cytometric analysis. The expression levels of epithelial‑mesenchymal transition (EMT)‑, apoptosis‑, and JNK signaling‑related proteins were detected by western blot analysis. Additionally, for rescue experiments, JNK signaling was activated following cell treatment with anisomycin. The results showed that GPX3 was downregulated in PC and its expression was associated with favorable prognosis. In addition, cell transfection‑induced GPX3 overexpression markedly inhibited cell proliferation, migration and invasion, and inhibited EMT. In addition, GPX3 improved the chemo‑sensitivity of PC and gemcitabine (GEM)‑resistant PC cells to GEM. Furthermore, GPX3 significantly suppressed JNK/c‑Jun signaling in PC, while anisomycin treatment reversed the inhibitory effects of GPX3 on the malignant behavior and chemo‑resistance of PC cells. The results of the present study indicated that GPX3 could serve as a tumor suppressor in PC via inhibiting JNK/c‑Jun signaling, thus providing novel insights into the treatment of PC.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Zhang L, Gao X and Zhu D: GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway. Exp Ther Med 27: 118, 2024.
APA
Ma, Y., Zhang, L., Gao, X., & Zhu, D. (2024). GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway. Experimental and Therapeutic Medicine, 27, 118. https://doi.org/10.3892/etm.2024.12407
MLA
Ma, Y., Zhang, L., Gao, X., Zhu, D."GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway". Experimental and Therapeutic Medicine 27.3 (2024): 118.
Chicago
Ma, Y., Zhang, L., Gao, X., Zhu, D."GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway". Experimental and Therapeutic Medicine 27, no. 3 (2024): 118. https://doi.org/10.3892/etm.2024.12407
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Zhang L, Gao X and Zhu D: GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway. Exp Ther Med 27: 118, 2024.
APA
Ma, Y., Zhang, L., Gao, X., & Zhu, D. (2024). GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway. Experimental and Therapeutic Medicine, 27, 118. https://doi.org/10.3892/etm.2024.12407
MLA
Ma, Y., Zhang, L., Gao, X., Zhu, D."GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway". Experimental and Therapeutic Medicine 27.3 (2024): 118.
Chicago
Ma, Y., Zhang, L., Gao, X., Zhu, D."GPX3 represses pancreatic cancer cell proliferation, migration and invasion, and improves their chemo‑sensitivity by regulating the JNK/c‑Jun signaling pathway". Experimental and Therapeutic Medicine 27, no. 3 (2024): 118. https://doi.org/10.3892/etm.2024.12407
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