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Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement

  • Authors:
    • Xue Ding
    • Lingyan Lai
    • Ying Jia
    • Xingyun Liu
    • Jia Hu
    • Wanlin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Orthodontics, School of Stomatology, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 121
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    Published online on: January 31, 2024
       https://doi.org/10.3892/etm.2024.12409
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Abstract

It has been reported that the force of orthodontic correction triggers periodontal tissue remodeling by affecting angiogenesis. However, the manifestation of the vascular response to orthodontic tooth movement in the setting of chronic fluorosis is unclear. The aim of the present study was to preliminarily explore the effect of orthodontic treatment on the angiogenesis of gingival tissue in rats with chronic fluorosis by monitoring changes in the expression of vascular endothelial growth factor (VEGF), phosphatidylinositol‑3 kinase (PI3K), AKT (or protein kinase B) and endothelial nitric oxide synthase (eNOS) in the gingival tissue. A total of 60 rats were randomly divided equally into the orthodontic group (O group; n=30) and fluorosis orthodontic group (FO group; n=30). Each of these groups was divided into 0‑, 3‑, 7‑, 14‑ and 21‑day groups (n=6/group). Fluorosis and orthodontic tooth movement models were established, and rats in each group were sacrificed for tissue sampling at the corresponding time points. Tissue morphology was observed via hematoxylin and eosin (H&E) staining. The protein and mRNA expression levels of VEGF, PI3K, AKT and eNOS in gingival tissue were detected by western blotting and reverse transcription‑quantitative polymerase chain reaction, respectively. The H&E staining images showed that the FO group had smaller blood vessels and reduced vascular proliferation compared with the O group. Furthermore, the mRNA and protein expression levels of VEGF, PI3K, AKT and eNOS were reduced in the gingiva of rats in the FO group compared with the O group, and certain reductions were significant during the delayed tooth movement period. In addition, with the extension of the application of orthodontic stress, the mRNA and protein expression levels of VEGF, PI3K, AKT and eNOS in the gingiva of the O and FO groups showed a trend of increasing at first and subsequently decreasing, which corresponds with the tooth movement cycle. In conclusion, chronic fluorosis may inhibit the angiogenesis and the expression of the VEGF/PI3K/AKT/eNOS pathway in gingival tissue of orthodontic tooth movement.
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Copy and paste a formatted citation
Spandidos Publications style
Ding X, Lai L, Jia Y, Liu X, Hu J and Chen W: Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement. Exp Ther Med 27: 121, 2024.
APA
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., & Chen, W. (2024). Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement. Experimental and Therapeutic Medicine, 27, 121. https://doi.org/10.3892/etm.2024.12409
MLA
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., Chen, W."Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement". Experimental and Therapeutic Medicine 27.3 (2024): 121.
Chicago
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., Chen, W."Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement". Experimental and Therapeutic Medicine 27, no. 3 (2024): 121. https://doi.org/10.3892/etm.2024.12409
Copy and paste a formatted citation
x
Spandidos Publications style
Ding X, Lai L, Jia Y, Liu X, Hu J and Chen W: Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement. Exp Ther Med 27: 121, 2024.
APA
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., & Chen, W. (2024). Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement. Experimental and Therapeutic Medicine, 27, 121. https://doi.org/10.3892/etm.2024.12409
MLA
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., Chen, W."Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement". Experimental and Therapeutic Medicine 27.3 (2024): 121.
Chicago
Ding, X., Lai, L., Jia, Y., Liu, X., Hu, J., Chen, W."Effects of chronic fluorosis on the expression of VEGF/PI3K/AKT/eNOS in the gingival tissue of rats with orthodontic tooth movement". Experimental and Therapeutic Medicine 27, no. 3 (2024): 121. https://doi.org/10.3892/etm.2024.12409
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