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Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation

  • Authors:
    • Aydin Yavuz
    • Ayşegül Küçük
    • Aydan İremnur Ergörün
    • Ali Doğan Dursun
    • Zeynep Yiğman
    • Metin Alkan
    • Mustafa Arslan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Faculty of Medicine, Gazi University, Ankara 06510, Turkey, Department of Physiology, Faculty of Medicine, Kutahya Health Science University, Kutahya 43020, Turkey, Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara 06510, Turkey, Department of Physiology, Faculty of Medicine, Atılım University, Ankara 06830, Turkey, Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara 06510, Turkey
    Copyright: © Yavuz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 242
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    Published online on: April 3, 2024
       https://doi.org/10.3892/etm.2024.12530
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Abstract

Sepsis is a systemic inflammatory response syndrome that develops in the host against microorganisms. This response develops away from the primary infection site and results in end‑organ damage. The present study aimed to investigate the protective and therapeutic effects on lung and kidney tissue of silymarin (S) and dexmedetomidine (DEX) applied 1 h before and after sepsis induced by the cecal ligation and puncture (CLP) method in rats. A total of 62 rats was randomly divided into eight groups: i) Control (n=6); ii) cecal perforation (CLP; n=8); iii) S + CLP (n=8; S + CLP; S administered 1 h before CPL); iv) CLP + S (n=8; S administered 1 h after CLP); v) DEX + CLP (n=8; D + CLP; DEX administered 1 h before CLP); vi) CLP + D (n=8; DEX administered 1 h after CLP); vii) SD + CLP (n=8; S and DEX administered 1 h before CLP) and viii) CLP + SD (n=8; S and DEX administered 1 h after CLP). After the cecum filled with stool, it was tied with 3/0 silk under the ileocecal valve and the anterior surface of the cecum was punctured twice with an 18‑gauge needle. A total of 100 mg/kg silymarin and 100 µg/kg DEX were administered intraperitoneally to the treatment groups. Lung and kidney tissue samples were collected to evaluate biochemical and histopathological parameters. In the histopathological examination, all parameters indicating kidney injury; interstitial edema, peritubular capillary dilatation, vacuolization, ablation of tubular epithelium from the basement membrane, loss of brush border in the proximal tubule epithelium, cell swelling and nuclear defragmentation; were increased in the CLP compared with the control group. Silymarin administration increased kidney damage, including ablation of tubular epithelium from the basement membrane, compared with that in the CLP group. DEX significantly reduced kidney damage compared with the CLP and silymarin groups. The co‑administration of DEX + silymarin decreased kidney damage, although it was not as effective as DEX‑alone. To conclude, intraperitoneal DEX ameliorated injury in CLP rats. DEX + silymarin partially ameliorated injury but silymarin administration increased damage. As a result, silymarin has a negative effects with this dosage and DEX has a protective effect. In the present study, it was determined that using the two drugs together had a greater therapeutic effect than silymarin and no differences in the effects were not observed any when the application times of the agents were changed.
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Copy and paste a formatted citation
Spandidos Publications style
Yavuz A, Küçük A, Ergörün AI, Dursun AD, Yiğman Z, Alkan M and Arslan M: Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation. Exp Ther Med 27: 242, 2024.
APA
Yavuz, A., Küçük, A., Ergörün, A.I., Dursun, A.D., Yiğman, Z., Alkan, M., & Arslan, M. (2024). Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation. Experimental and Therapeutic Medicine, 27, 242. https://doi.org/10.3892/etm.2024.12530
MLA
Yavuz, A., Küçük, A., Ergörün, A. I., Dursun, A. D., Yiğman, Z., Alkan, M., Arslan, M."Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation". Experimental and Therapeutic Medicine 27.6 (2024): 242.
Chicago
Yavuz, A., Küçük, A., Ergörün, A. I., Dursun, A. D., Yiğman, Z., Alkan, M., Arslan, M."Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation". Experimental and Therapeutic Medicine 27, no. 6 (2024): 242. https://doi.org/10.3892/etm.2024.12530
Copy and paste a formatted citation
x
Spandidos Publications style
Yavuz A, Küçük A, Ergörün AI, Dursun AD, Yiğman Z, Alkan M and Arslan M: Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation. Exp Ther Med 27: 242, 2024.
APA
Yavuz, A., Küçük, A., Ergörün, A.I., Dursun, A.D., Yiğman, Z., Alkan, M., & Arslan, M. (2024). Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation. Experimental and Therapeutic Medicine, 27, 242. https://doi.org/10.3892/etm.2024.12530
MLA
Yavuz, A., Küçük, A., Ergörün, A. I., Dursun, A. D., Yiğman, Z., Alkan, M., Arslan, M."Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation". Experimental and Therapeutic Medicine 27.6 (2024): 242.
Chicago
Yavuz, A., Küçük, A., Ergörün, A. I., Dursun, A. D., Yiğman, Z., Alkan, M., Arslan, M."Evaluation of the efficacy of silymarin and dexmedetomidine on kidney and lung tissue in the treatment of sepsis in rats with cecal perforation". Experimental and Therapeutic Medicine 27, no. 6 (2024): 242. https://doi.org/10.3892/etm.2024.12530
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