Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2024 Volume 28 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2024 Volume 28 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis

  • Authors:
    • Ao Xuan Zhen
    • Kyoung Ah Kang
    • Mei Jing Piao
    • Pincha Devage Sameera Madushan Fernando
    • Herath Mudiyanselage Udari Lakmini Herath
    • Jin Won Hyun
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, College of Medicine and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea
    Copyright: © Zhen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 275
    |
    Published online on: May 8, 2024
       https://doi.org/10.3892/etm.2024.12563
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Particulate matter 2.5 (PM2.5) imposes a heavy burden on the skin and respiratory system of human beings, causing side effects such as aging, inflammation and cancer. Astaxanthin (ATX) is a well‑known antioxidant widely used for its anti‑inflammatory and anti‑aging properties. However, few studies have investigated the protective effects of ATX against PM2.5‑induced senescence in HaCaT cells. In the present study, the levels of reactive oxygen species (ROS) and antioxidant enzymes were measured after treatment with PM2.5. The results revealed that PM2.5 generated excessive ROS and reduced the translocation of nuclear factor erythroid 2‑related factor 2 (NRF2), subsequently reducing the expression of antioxidant enzymes. However, pretreatment with ATX reversed the ROS levels as well as the expression of antioxidant enzymes. In addition, ATX protected cells from PM2.5‑induced DNA damage and rescued PM2.5‑induced cell cycle arrest. The levels of senescence‑associated phenotype markers, such as interleukin‑1β, matrix metalloproteinases, and β‑galactosidase, were increased by exposure to PM2.5, however these effects were reversed by ATX. After interfering with NRF2 mRNA expression and exposing cells to PM2.5, the levels of ROS and β‑galactosidase were higher compared with siControl RNA cells exposed to PM2.5. However, ATX inhibited ROS and β‑galactosidase levels in both the siControl RNA and the siNRF2 RNA groups. Thus, ATX protects HaCaT keratinocytes from PM2.5‑induced senescence by partially inhibiting excessive ROS generation via the NRF2 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Lelieveld J, Evans JS, Fnais M, Giannadaki D and Pozzer A: The contribution of outdoor air pollution sources to premature mortality on a global scale. Nature. 525:367–371. 2015.PubMed/NCBI View Article : Google Scholar

2 

Boogaard H, Patton AP, Atkinson RW, Brook JR, Chang HH, Crouse DL, Fussell JC, Hoek G, Hoffmann B, Kappeler R, et al: Long-term exposure to traffic-related air pollution and selected health outcomes: A systematic review and meta-analysis. Environ Int. 164(107262)2022.PubMed/NCBI View Article : Google Scholar

3 

Ryu YS, Kang KA, Piao MJ, Ahn MJ, Yi JM, Hyun YM, Kim SH, Ko MK, Park CO and Hyun JW: Particulate matter induces inflammatory cytokine production via activation of NFκB by TLR5-NOX4-ROS signaling in human skin keratinocyte and mouse skin. Redox Biol. 21(101080)2019.PubMed/NCBI View Article : Google Scholar

4 

Piao MJ, Ahn MJ, Kang KA, Ryu YS, Hyun YJ, Shilnikova K, Zhen AX, Jeong JW, Choi YH, Kang HK, et al: Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis. Arch Toxicol. 92:2077–2091. 2018.PubMed/NCBI View Article : Google Scholar

5 

Hyun YJ, Piao MJ, Kang KA, Zhen AX, Madushan Fernando PDS, Kang HK, Ahn YS and Hyun JW: Effect of fermented fish oil on fine particulate matter-induced skin aging. Mar Drugs. 17(61)2019.PubMed/NCBI View Article : Google Scholar

6 

Reynolds WJ, Hanson PS, Critchley A, Griffiths B, Chavan B and Birch-Machin MA: Exposing human primary dermal fibroblasts to particulate matter induces changes associated with skin aging. FASEB J. 34:14725–14735. 2020.PubMed/NCBI View Article : Google Scholar

7 

Mendonça ELSS, Xavier JA, Fragoso MBT, Silva MO, Escodro PB, Oliveira ACM, Tucci P, Saso L and Goulart MOF: E-stilbenes, general chemical and biological aspects, potential pharmacological activity based on the Nrf2 pathway. Pharmaceuticals (Basel). 17(232)2024.PubMed/NCBI View Article : Google Scholar

8 

Zhang LM, Lv SS, Fu SR, Wang JQ, Liang LY, Li RQ, Zhang F and Ma YX: Procyanidins inhibit fine particulate matter-induced vascular smooth muscle cells apoptosis via the activation of the Nrf2 signaling pathway. Ecotoxicol Environ Saf. 223(112586)2021.PubMed/NCBI View Article : Google Scholar

9 

Kahremany S, Hofmann L, Eretz-Kdosha N, Silberstein E, Gruzman A and Cohen G: SH-29 and SK-119 attenuates air-pollution induced damage by activating Nrf2 in HaCaT cells. Int J Environ Res Public Health. 18(12371)2021.PubMed/NCBI View Article : Google Scholar

10 

Han SI, Chang SH, Lee C, Jeon MS, Heo YM, Kim S and Choi YE: Astaxanthin biosynthesis promotion with pH shock in the green microalga, Haematococcus lacustris. Bioresour Technol. 314(123725)2020.PubMed/NCBI View Article : Google Scholar

11 

Kumar S, Kumar R, Diksh Kumari A and Panwar A: Astaxanthin: A super antioxidant from microalgae and its therapeutic potential. J Basic Microbiol. 62:1064–1082. 2022.PubMed/NCBI View Article : Google Scholar

12 

Ashrafizadeh M, Ahmadi Z, Yaribeygi H, Sathyapalan T and Sahebkar A: Astaxanthin and Nrf2 signaling pathway: A novel target for new therapeutic approaches. Mini Rev Med Chem. 22:312–321. 2022.PubMed/NCBI View Article : Google Scholar

13 

Imokawa G: Intracellular signaling mechanisms involved in the biological effects of the xanthophyll carotenoid astaxanthin to prevent the photo-aging of the skin in a reactive oxygen species depletion-independent manner: The key role of mitogen and stress-activated protein kinase 1. Photochem Photobiol. 95:480–489. 2019.PubMed/NCBI View Article : Google Scholar

14 

Zhen AX, Piao MJ, Hyun YJ, Kang KA, Madushan Fernando PDS, Cho SJ, Ahn MJ and Hyun JW: Diphlorethohydroxycarmalol attenuates fine particulate matter-induced subcellular skin dysfunction. Mar Drugs. 17(95)2019.PubMed/NCBI View Article : Google Scholar

15 

Matthews HK, Bertoli C and de Bruin RAM: Cell cycle control in cancer. Nat Rev Mol Cell Biol. 23:74–88. 2022.PubMed/NCBI View Article : Google Scholar

16 

Hernandez-Segura A, Nehme J and Demaria M: Hallmarks of cellular senescence. Trends Cell Biol. 28:436–453. 2018.PubMed/NCBI View Article : Google Scholar

17 

Oh JH, Joo YH, Karadeniz F, Ko J and Kong CS: Syringaresinol inhibits UVA-induced MMP-1 expression by suppression of MAPK/AP-1 signaling in HaCaT keratinocytes and human dermal fibroblasts. Int J Mol Sci. 21(3981)2020.PubMed/NCBI View Article : Google Scholar

18 

Samdavid Thanapaul RJR, Shvedova M, Shin GH, Crouch J and Roh DS: Elevated skin senescence in young mice causes delayed wound healing. Geroscience. 44:1871–1878. 2022.PubMed/NCBI View Article : Google Scholar

19 

Ryu YS, Kang KA, Piao MJ, Ahn MJ, Yi JM, Bossis G, Hyun YM, Park CO and Hyun JW: Particulate matter-induced senescence of skin keratinocytes involves oxidative stress-dependent epigenetic modifications. Exp Mol Med. 51:1–14. 2019.PubMed/NCBI View Article : Google Scholar

20 

Zhen AX, Piao MJ, Kang KA, Fernando PD, Herath HM, Cho SJ and Hyun JW: 3-Bromo-4,5-dihydroxybenzaldehyde protects keratinocytes from particulate matter 2.5-induced damages. Antioxidants (Basel). 12(1307)2023.PubMed/NCBI View Article : Google Scholar

21 

Wang L, Lee W, Jayawardena TU, Cha SH and Jeon YJ: Dieckol, an algae-derived phenolic compound, suppresses airborne particulate matter-induced skin aging by inhibiting the expressions of pro-inflammatory cytokines and matrix metalloproteinases through regulating NF-κB, AP-1, and MAPKs signaling pathways. Food Chem Toxicol. 146(111823)2020.PubMed/NCBI View Article : Google Scholar

22 

Ma C, Yang K, Wang Y and Dai X: Anti-aging effect of agar oligosaccharide on male Drosophila melanogaster and its preliminary mechanism. Mar Drugs. 17(632)2019.PubMed/NCBI View Article : Google Scholar

23 

Liu XY, Liu D, Lin GP, Wu YJ, Gao LY, Ai C, Huang YF, Wang MF, El-Seedi HR, Chen XH and Zhao C: Anti-ageing and antioxidant effects of sulfate oligosaccharides from green algae Ulva lactuca and Enteromorpha prolifera in SAMP8 mice. Int J Biol Macromol. 139:342–351. 2019.PubMed/NCBI View Article : Google Scholar

24 

Sorrenti V, Davinelli S, Scapagnini G, Willcox BJ, Allsopp RC and Willcox DC: Astaxanthin as a putative geroprotector: Molecular basis and focus on brain aging. Mar Drugs. 18(351)2020.PubMed/NCBI View Article : Google Scholar

25 

Chen Z, Xiao J, Liu H, Yao K, Hou X, Cao Y and Liu X: Astaxanthin attenuates oxidative stress and immune impairment in D-galactose-induced aging in rats by activating the Nrf2/Keap1 pathway and suppressing the NF-κB pathway. Food Funct. 11:8099–8111. 2020.PubMed/NCBI View Article : Google Scholar

26 

Herath HMUL, Piao MJ, Kang KA, Zhen AX, Fernando PDSM, Kang HK, Yi JM and Hyun JW: Hesperidin exhibits protective effects against PM2.5-mediated mitochondrial damage, cell cycle arrest, and cellular senescence in human HaCaT keratinocytes. Molecules. 27(4800)2022.PubMed/NCBI View Article : Google Scholar

27 

Kumari R and Jat P: Mechanisms of cellular senescence: Cell cycle arrest and senescence associated secretory phenotype. Front Cell Dev Biol. 9(645593)2021.PubMed/NCBI View Article : Google Scholar

28 

Zhou Q, Wang W, Wu J, Qiu S, Yuan S, Fu PL, Qian QR and Xu YZ: Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered. 13:2017–2027. 2022.PubMed/NCBI View Article : Google Scholar

29 

Chen L, Yang R, Qiao W, Zhang W, Chen J, Mao L, Goltzman D and Miao D: 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling. Aging Cell. 18(e12951)2019.PubMed/NCBI View Article : Google Scholar

30 

Lee JJ, Ng SC, Hsu JY, Liu H, Chen CJ, Huang CY and Kuo WW: Galangin reverses H2O2-induced dermal fibroblast senescence via SIRT1-PGC-1α/Nrf2 signaling. Int J Mol Sci. 23(1387)2022.PubMed/NCBI View Article : Google Scholar

31 

Kumar N, Reddi S, Devi S, Mada SB, Kapila R and Kapila S: Nrf2 dependent antiaging effect of milk-derived bioactive peptide in old fibroblasts. J Cell Biochem. 120:9677–9691. 2019.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhen AX, Kang KA, Piao MJ, Madushan Fernando PS, Lakmini Herath HU and Hyun JW: Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis. Exp Ther Med 28: 275, 2024.
APA
Zhen, A.X., Kang, K.A., Piao, M.J., Madushan Fernando, P.S., Lakmini Herath, H.U., & Hyun, J.W. (2024). Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis. Experimental and Therapeutic Medicine, 28, 275. https://doi.org/10.3892/etm.2024.12563
MLA
Zhen, A. X., Kang, K. A., Piao, M. J., Madushan Fernando, P. S., Lakmini Herath, H. U., Hyun, J. W."Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis". Experimental and Therapeutic Medicine 28.1 (2024): 275.
Chicago
Zhen, A. X., Kang, K. A., Piao, M. J., Madushan Fernando, P. S., Lakmini Herath, H. U., Hyun, J. W."Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis". Experimental and Therapeutic Medicine 28, no. 1 (2024): 275. https://doi.org/10.3892/etm.2024.12563
Copy and paste a formatted citation
x
Spandidos Publications style
Zhen AX, Kang KA, Piao MJ, Madushan Fernando PS, Lakmini Herath HU and Hyun JW: Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis. Exp Ther Med 28: 275, 2024.
APA
Zhen, A.X., Kang, K.A., Piao, M.J., Madushan Fernando, P.S., Lakmini Herath, H.U., & Hyun, J.W. (2024). Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis. Experimental and Therapeutic Medicine, 28, 275. https://doi.org/10.3892/etm.2024.12563
MLA
Zhen, A. X., Kang, K. A., Piao, M. J., Madushan Fernando, P. S., Lakmini Herath, H. U., Hyun, J. W."Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis". Experimental and Therapeutic Medicine 28.1 (2024): 275.
Chicago
Zhen, A. X., Kang, K. A., Piao, M. J., Madushan Fernando, P. S., Lakmini Herath, H. U., Hyun, J. W."Protective effects of astaxanthin on particulate matter 2.5‑induced senescence in HaCaT keratinocytes via maintenance of redox homeostasis". Experimental and Therapeutic Medicine 28, no. 1 (2024): 275. https://doi.org/10.3892/etm.2024.12563
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team