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Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms

  • Authors:
    • Lei Yan
    • Chu Chen
    • Lingling Wang
    • Hongxiang Hong
    • Chunshuai Wu
    • Jiayi Huang
    • Jiawei Jiang
    • Jiajia Chen
    • Guanhua Xu
    • Zhiming Cui
  • View Affiliations / Copyright

    Affiliations: The First People's Hospital of Nantong, The Second Affiliated Hospital of Nantong University, Research Institute for Spine and Spinal Cord Disease of Nantong University, Nantong, Jiangsu 226019, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 292
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    Published online on: May 22, 2024
       https://doi.org/10.3892/etm.2024.12581
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Abstract

Spinal cord injury (SCI) is a severe neurological complication following spinal fracture, which has long posed a challenge for clinicians. Microglia play a dual role in the pathophysiological process after SCI, both beneficial and detrimental. The underlying mechanisms of microglial actions following SCI require further exploration. The present study combined three different machine learning algorithms, namely weighted gene co‑expression network analysis, random forest analysis and least absolute shrinkage and selection operator analysis, to screen for differentially expressed genes in the GSE96055 microglia dataset after SCI. It then used protein‑protein interaction networks and gene set enrichment analysis with single genes to investigate the key genes and signaling pathways involved in microglial function following SCI. The results indicated that microglia not only participate in neuroinflammation but also serve a significant role in the clearance mechanism of apoptotic cells following SCI. Notably, bioinformatics analysis and lipopolysaccharide + UNC569 (a MerTK‑specific inhibitor) stimulation of BV2 cell experiments showed that the expression levels of Anxa2, Myo1e and Spp1 in microglia were significantly upregulated following SCI, thus potentially involved in regulating the clearance mechanism of apoptotic cells. The present study suggested that Anxa2, Myo1e and Spp1 may serve as potential targets for the future treatment of SCI and provided a theoretical basis for the development of new methods and drugs for treating SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Yan L, Chen C, Wang L, Hong H, Wu C, Huang J, Jiang J, Chen J, Xu G, Cui Z, Cui Z, et al: Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms. Exp Ther Med 28: 292, 2024.
APA
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J. ... Cui, Z. (2024). Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms. Experimental and Therapeutic Medicine, 28, 292. https://doi.org/10.3892/etm.2024.12581
MLA
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J., Jiang, J., Chen, J., Xu, G., Cui, Z."Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms". Experimental and Therapeutic Medicine 28.1 (2024): 292.
Chicago
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J., Jiang, J., Chen, J., Xu, G., Cui, Z."Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms". Experimental and Therapeutic Medicine 28, no. 1 (2024): 292. https://doi.org/10.3892/etm.2024.12581
Copy and paste a formatted citation
x
Spandidos Publications style
Yan L, Chen C, Wang L, Hong H, Wu C, Huang J, Jiang J, Chen J, Xu G, Cui Z, Cui Z, et al: Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms. Exp Ther Med 28: 292, 2024.
APA
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J. ... Cui, Z. (2024). Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms. Experimental and Therapeutic Medicine, 28, 292. https://doi.org/10.3892/etm.2024.12581
MLA
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J., Jiang, J., Chen, J., Xu, G., Cui, Z."Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms". Experimental and Therapeutic Medicine 28.1 (2024): 292.
Chicago
Yan, L., Chen, C., Wang, L., Hong, H., Wu, C., Huang, J., Jiang, J., Chen, J., Xu, G., Cui, Z."Analysis of gene expression in microglial apoptotic cell clearance following spinal cord injury based on machine learning algorithms". Experimental and Therapeutic Medicine 28, no. 1 (2024): 292. https://doi.org/10.3892/etm.2024.12581
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