Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2024 Volume 28 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2024 Volume 28 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer

  • Authors:
    • Jianping Jiang
    • Weiqiang Mo
    • Xue Lian
    • Dakui Cao
    • Haiying Cheng
    • Haiqin Wang
  • View Affiliations

    Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314000, P.R. China, Department of Nursing Administration, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314000, P.R. China
  • Published online on: May 22, 2024     https://doi.org/10.3892/etm.2024.12583
  • Article Number: 294
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to assess the roles of peripheral circulating tumor cell (CTC) count, CTC subtypes and programmed death ligand 1 (PD‑L1) expression in the clinical staging and prognosis of patients with non‑small cell lung cancer (NSCLC). A total of 100 patients with NSCLC with available tumor tissues were enrolled in the present study, and 7.5 ml peripheral blood was collected. Patients were divided into PD‑L1‑positive and PD‑L1‑negative groups according to PD‑L1 immunohistochemical staining. Peripheral blood samples from both groups were analyzed to determine the CTC count, epithelial‑type CTCs (E‑CTCs), mesenchymal‑type CTCs (M‑CTCs) and PD‑L1 expression. Clinical data were collected, and patients were followed up for a maximum of 36 months, with patient death as the endpoint event. Patients with PD‑L1‑positive tumors had a worse prognosis compared with those with PD‑L1‑negative tumors (P=0.045). The PD‑L1‑positive group exhibited significantly higher numbers of CTCs and M‑CTCs compared with the PD‑L1‑negative group (P≤0.05). However, the number of E‑CTCs did not differ significantly between the two groups (P>0.05). PD‑L1‑positive patients with higher CTC and M‑CTC counts had relatively poorer prognoses (P≤0.05), while the number of E‑CTCs had no significant effect on prognosis (P>0.05). Compared with the early‑stage NSCLC group, the late‑stage NSCLC group exhibited a significant increase in the CTC count (P≤0.05), while E‑CTC and M‑CTC counts did not significantly differ between the two groups (P>0.05). The PD‑L1‑positive group exhibited a significant increase in the number of PD‑L1+ CTCs and PD‑L1+ M‑CTCs compared with the PD‑L1‑negative group (P≤0.05), while PD‑L1+ E‑CTC counts did not differ significantly between the two groups (P>0.05). The PD‑L1‑positive patients with a higher number of PD‑L1+ CTCs and PD‑L1+ M‑CTCs had relatively poorer prognoses (P≤0.05), while the PD‑L1+ E‑CTC count had no significant effect on prognosis (P>0.05). Compared with the early‑stage NSCLC group, the late‑stage NSCLC group exhibited a significant increase in the number of PD‑L1+ CTCs and PD‑L1+ M‑CTCs (P≤0.05), while PD‑L1+ E‑CTC counts did not significantly differ between the two groups (P>0.05). Based on univariate and multivariate analyses, the number of PD‑L1+ M‑CTCs was identified as an independent prognostic factor for NSCLC. In conclusion, the presence of CTCs in peripheral blood, particularly PD‑L1+ M‑CTC subtype, indicated poorer clinical staging and prognosis in patients with NSCLC. These findings suggested that CTCs, specifically the PD‑L1+ M‑CTC subtype, could serve as a monitoring indicator for the clinical staging and prognosis of patients with NSCLC.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Adjei AA: Lung cancer worldwide. J Thorac Oncol. 14(956)2019.PubMed/NCBI View Article : Google Scholar

2 

Thai AA, Solomon BJ, Sequist LV, Gainor JF and Heist RS: Lung cancer. Lancet. 398:535–554. 2021.PubMed/NCBI View Article : Google Scholar

3 

Adams SJ, Stone E, Baldwin DR, Vliegenthart R, Lee P and Fintelmann FJ: Lung cancer screening. Lancet. 401:390–408. 2023.PubMed/NCBI View Article : Google Scholar

4 

Yu Y, Zeng D, Ou Q, Liu S, Li A, Chen Y, Lin D, Gao Q, Zhou H, Liao W and Yao H: Association of survival and Immune-related biomarkers with immunotherapy in patients with non-small cell lung cancer: A Meta-analysis and individual patient-level analysis. JAMA Netw Open. 2(e196879)2019.PubMed/NCBI View Article : Google Scholar

5 

Steven A, Fisher SA and Robinson BW: Immunotherapy for lung cancer. Respirology. 21:821–833. 2016.PubMed/NCBI View Article : Google Scholar

6 

Rupp B, Ball H, Wuchu F, Nagrath D and Nagrath S: Circulating tumor cells in precision medicine: Challenges and opportunities. Trends Pharmacol Sci. 43:378–391. 2022.PubMed/NCBI View Article : Google Scholar

7 

Aceto N: Bring along your friends: Homotypic and heterotypic circulating tumor cell clustering to accelerate metastasis. Biomed J. 43:18–23. 2020.PubMed/NCBI View Article : Google Scholar

8 

Gires O, Pan M, Schinke H, Canis M and Baeuerle PA: Expression and function of epithelial cell adhesion molecule EpCAM: Where are we after 40 years? Cancer Metastasis Rev. 39:969–987. 2020.PubMed/NCBI View Article : Google Scholar

9 

Krebs MG, Hou JM, Sloane R, Lancashire L, Priest L, Nonaka D, Ward TH, Backen A, Clack G, Hughes A, et al: Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and -independent approaches. J Thorac Oncol. 7:306–315. 2012.PubMed/NCBI View Article : Google Scholar

10 

Lin D, Shen L, Luo M, Zhang K, Li J, Yang Q, Zhu F, Zhou D, Zheng S, Chen Y and Zhou J: Circulating tumor cells: Biology and clinical significance. Signal Transduct Target Ther. 6(404)2021.PubMed/NCBI View Article : Google Scholar

11 

Bae SY, Kamalanathan KJ, Galeano-Garces C, Konety BR, Antonarakis ES, Parthasarathy J, Hong J and Drake JM: Dissemination of circulating tumor cells in breast and prostate cancer: Implications for early detection. Endocrinology. 165(bqae022)2024.PubMed/NCBI View Article : Google Scholar

12 

Mitra A, Mishra L and Li SL: EMT, CTCs and CSCs in tumor relapse and drug-resistance. Oncotarget. 6:10697–10711. 2015.PubMed/NCBI View Article : Google Scholar

13 

Joosse SA, Gorges TM and Pantel K: Biology, detection, and clinical implications of circulating tumor cells. Embo Mol Med. 7:1–11. 2015.PubMed/NCBI View Article : Google Scholar

14 

Zhang X, Wei L, Li J, Zheng J, Zhang S and Zhou J: Epithelial-mesenchymal transition phenotype of circulating tumor cells is associated with distant metastasis in patients with NSCLC. Mol Med Rep. 19:601–608. 2019.PubMed/NCBI View Article : Google Scholar

15 

Zhang Y, Men Y, Wang J, Xing P, Zhao J, Li J, Xu D, Hui Z and Cui W: Epithelial circulating tumor cells with a heterogeneous phenotype are associated with metastasis in NSCLC. J Cancer Res Clin Oncol. 148:1137–1146. 2022.PubMed/NCBI View Article : Google Scholar

16 

Pantazaka E, Vardas V, Roumeliotou A, Kakavogiannis S and Kallergi G: Clinical relevance of mesenchymal- and stem-associated phenotypes in circulating tumor cells isolated from lung cancer patients. Cancers (Basel). 13(2158)2021.PubMed/NCBI View Article : Google Scholar

17 

Bie F, Tian H, Sun N, Zang R, Zhang M, Song P, Liu L, Peng Y, Bai G, Zhou B and Gao S: Research progress of Anti-PD-1/PD-L1 immunotherapy related mechanisms and predictive biomarkers in NSCLC. Front Oncol. 12(769124)2022.PubMed/NCBI View Article : Google Scholar

18 

Hu CB, Huang C, Wang J, Hong Y, Fan DD, Chen Y, Lin AF, Xiang LX and Shao JZ: PD-L1/BTLA checkpoint axis exploited for bacterial immune escape by restraining CD8+ T Cell-initiated adaptive immunity in Zebrafish. J Immunol. 211:816–835. 2023.PubMed/NCBI View Article : Google Scholar

19 

Juneja VR, McGuire KA, Manguso RT, LaFleur MW, Collins N, Haining WN, Freeman GJ and Sharpe AH: PD-L1 on tumor cells is sufficient for immune evasion in immunogenic tumors and inhibits CD8 T cell cytotoxicity. J Exp Med. 214:895–904. 2017.PubMed/NCBI View Article : Google Scholar

20 

Brody R, Zhang Y, Ballas M, Siddiqui MK, Gupta P, Barker C, Midha A and Walker J: PD-L1 expression in advanced NSCLC: Insights into risk stratification and treatment selection from a systematic literature review. Lung Cancer. 112:200–215. 2017.PubMed/NCBI View Article : Google Scholar

21 

Barclay J, Creswell J and León J: Cancer immunotherapy and the PD-1/PD-L1 checkpoint pathway. Arch Esp Urol. 71:393–399. 2018.PubMed/NCBI

22 

Ligero M, Serna G, El Nahhas OSM, Sansano I, Mauchanski S, Viaplana C, Calderaro J, Toledo RA, Dienstmann R, Vanguri RS, et al: Weakly supervised deep learning predicts immunotherapy response in solid tumors based on PD-L1 expression. Cancer Res Commun. 4:92–102. 2024.PubMed/NCBI View Article : Google Scholar

23 

Hou F, Qi C, Lu Y, Li F and Hao Z: Cell HE staining smears and paired cell paraffin sections in detection of epithelial growth factor receptor gene of pleural fluid specimens. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 47:35–44. 2022.PubMed/NCBI View Article : Google Scholar : (In English, Chinese).

24 

Davies CR, Guo T, Burke E, Stankiewicz E, Xu L, Mao X, Scandura G, Rajan P, Tipples K, Alifrangis C, et al: The potential of using circulating tumour cells and their gene expression to predict docetaxel response in metastatic prostate cancer. Front Oncol. 12(1060864)2023.PubMed/NCBI View Article : Google Scholar

25 

Castro-Giner F and Aceto N: Tracking cancer progression: From circulating tumor cells to metastasis. Genome Med. 12(31)2020.PubMed/NCBI View Article : Google Scholar

26 

Zhang H, Yuan F, Qi Y, Liu B and Chen Q: Circulating tumor cells for Glioma. Front Oncol. 11(607150)2021.PubMed/NCBI View Article : Google Scholar

27 

Liang DH, Hall C and Lucci A: Circulating tumor cells in breast cancer. Recent Results Cancer Res. 215:127–145. 2020.PubMed/NCBI View Article : Google Scholar

28 

Pantel K: Circulating tumor cells in head and neck carcinomas. Clin Chem. 65:1193–1195. 2019.PubMed/NCBI View Article : Google Scholar

29 

Massari F, Di Nunno V, Comito F, Cubelli M, Ciccarese C, Iacovelli R, Fiorentino M, Montironi R and Ardizzoni A: Circulating tumor cells in genitourinary tumors. Ther Adv Urol. 10:65–77. 2018.PubMed/NCBI View Article : Google Scholar

30 

Castello A, Carbone FG, Rossi S, Monterisi S, Federico D, Toschi L and Lopci E: Circulating tumor cells and metabolic parameters in NSCLC patients treated with checkpoint inhibitors. Cancers (Basel). 12(487)2020.PubMed/NCBI View Article : Google Scholar

31 

Chen M, Xu R, Wu L and Chen X: Relationship between circulating tumor cells undergoing EMT and short-term efficacy following interventional treatment in patients with hepatocellular carcinoma. J Interv Med. 3:146–150. 2020.PubMed/NCBI View Article : Google Scholar

32 

Thunnissen E, de Langen AJ and Smit EF: PD-L1 IHC in NSCLC with a global and methodological perspective. Lung Cancer. 113:102–105. 2017.PubMed/NCBI View Article : Google Scholar

33 

Wang Y, Kim TH, Fouladdel S, Zhang Z, Soni P, Qin A, Zhao L, Azizi E, Lawrence TS, Ramnath N, et al: PD-L1 expression in circulating tumor cells increases during Radio(chemo)therapy and indicates poor prognosis in Non-small cell lung cancer. Sci Rep. 9(566)2019.PubMed/NCBI View Article : Google Scholar

34 

Cheng Y, Wang T, Lv X, Li R, Yuan L, Shen J, Li Y, Yan T, Liu B and Wang L: Detection of PD-L1 expression and its clinical significance in circulating tumor cells from patients with Non-small-cell lung cancer. Cancer Manag Res. 12:2069–2078. 2020.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jiang J, Mo W, Lian X, Cao D, Cheng H and Wang H: Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer. Exp Ther Med 28: 294, 2024.
APA
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., & Wang, H. (2024). Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer. Experimental and Therapeutic Medicine, 28, 294. https://doi.org/10.3892/etm.2024.12583
MLA
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., Wang, H."Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer". Experimental and Therapeutic Medicine 28.1 (2024): 294.
Chicago
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., Wang, H."Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer". Experimental and Therapeutic Medicine 28, no. 1 (2024): 294. https://doi.org/10.3892/etm.2024.12583
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang J, Mo W, Lian X, Cao D, Cheng H and Wang H: Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer. Exp Ther Med 28: 294, 2024.
APA
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., & Wang, H. (2024). Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer. Experimental and Therapeutic Medicine, 28, 294. https://doi.org/10.3892/etm.2024.12583
MLA
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., Wang, H."Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer". Experimental and Therapeutic Medicine 28.1 (2024): 294.
Chicago
Jiang, J., Mo, W., Lian, X., Cao, D., Cheng, H., Wang, H."Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer". Experimental and Therapeutic Medicine 28, no. 1 (2024): 294. https://doi.org/10.3892/etm.2024.12583
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team