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Article Open Access

Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis

  • Authors:
    • Chen Yue
    • Maoxiao Ma
    • Jiayi Guo
    • Hongjun Li
    • Yuxia Yang
    • Youwen Liu
    • Bin Xu
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    Affiliations: Evidence Based Medicine Center, Luoyang Orthopedic‑Traumatological Hospital of Henan Province, Luoyang, Henan 471002, P.R. China, Department of Orthopedics, Luoyang Orthopedic‑Traumatological Hospital of Henan Province, Luoyang, Henan 471002, P.R. China, Department of Orthopedics, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, P.R. China
    Copyright: © Yue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 311
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    Published online on: June 5, 2024
       https://doi.org/10.3892/etm.2024.12599
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Abstract

Excessive alcohol consumption is considered to be a major risk factor of alcohol‑induced osteonecrosis of the femoral head (AONFH). The gut microbiota (GM) has been reported to aid in the regulation of human physiology and its composition can be altered by alcohol consumption. The aim of the present study was to improve the understanding of the GM and its metabolites in patients with AONFH. Metabolomic sequencing and 16S rDNA analysis of fecal samples were performed using liquid chromatography‑mass spectrometry to characterize the GM of patients with AONFH and healthy normal controls (NCs). Metagenomic sequencing of fecal samples was performed to identify whether GM changes on the species level were associated with the expression of gut bacteria genes or their associated functions in patients with AONFH. The abundance of 58 genera was found to differ between the NC group and the AONFH group. Specifically, Klebsiella, Holdemanella, Citrobacter and Lentilactobacillus were significantly more abundant in the AONFH group compared with those in the NC group. Metagenomic sequencing demonstrated that the majority of the bacterial species that exhibited significantly different abundance in patients with AONFH belonged to the genus Pseudomonas. Fecal metabolomic analysis demonstrated that several metabolites were present at significantly different concentrations in the AONFH group compared with those in the NC group. These metabolites were products of vitamin B6 metabolism, retinol metabolism, pentose and glucuronate interconversions and glycerophospholipid metabolism. In addition, these changes in metabolite levels were observed to be associated with the altered abundance of specific bacterial species, such as Basidiobolus, Mortierella, Phanerochaete and Ceratobasidium. According to the results of the present study, a comprehensive landscape of the GM and metabolites in patients with AONFH was revealed, suggesting the existence of interplay between the gut microbiome and metabolome in AONFH pathogenesis.
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Spandidos Publications style
Yue C, Ma M, Guo J, Li H, Yang Y, Liu Y and Xu B: Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis. Exp Ther Med 28: 311, 2024.
APA
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., & Xu, B. (2024). Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis. Experimental and Therapeutic Medicine, 28, 311. https://doi.org/10.3892/etm.2024.12599
MLA
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., Xu, B."Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis". Experimental and Therapeutic Medicine 28.2 (2024): 311.
Chicago
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., Xu, B."Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis". Experimental and Therapeutic Medicine 28, no. 2 (2024): 311. https://doi.org/10.3892/etm.2024.12599
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Spandidos Publications style
Yue C, Ma M, Guo J, Li H, Yang Y, Liu Y and Xu B: Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis. Exp Ther Med 28: 311, 2024.
APA
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., & Xu, B. (2024). Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis. Experimental and Therapeutic Medicine, 28, 311. https://doi.org/10.3892/etm.2024.12599
MLA
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., Xu, B."Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis". Experimental and Therapeutic Medicine 28.2 (2024): 311.
Chicago
Yue, C., Ma, M., Guo, J., Li, H., Yang, Y., Liu, Y., Xu, B."Altered gut microbe metabolites in patients with alcohol‑induced osteonecrosis of the femoral head: An integrated omics analysis". Experimental and Therapeutic Medicine 28, no. 2 (2024): 311. https://doi.org/10.3892/etm.2024.12599
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