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Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress

  • Authors:
    • Qiongyu Li
    • Wan-Rong Shi
    • Yun-Lin Huang
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    Affiliations: College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, Fujian 362000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 310
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    Published online on: June 5, 2024
       https://doi.org/10.3892/etm.2024.12600
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Abstract

Chitin degradation products, especially chitosan oligosaccharides (COSs), are highly valued in various industrial fields, such as food, medicine, cosmetics and agriculture, for their rich resources and high cost‑effectiveness. However, little is known about the impact of acetylation on COS cellular bioactivity. The present study aimed to compare the differential effects of COS and highly N‑acetylated COS (NACOS), known as chitin oligosaccharide, on H2O2‑induced cell stress. MTT assay showed that pretreatment with NACOS and COS markedly inhibited H2O2‑induced RAW264.7 cell death in a concentration‑dependent manner. Flow cytometry indicated that NACOS and COS exerted an anti‑apoptosis effect on H2O2‑induced oxidative damage in RAW264.7 cells. NACOS and COS treatment ameliorated H2O2‑induced RAW264.7 cell cycle arrest. Western blotting revealed that the anti‑oxidation effects of NACOS and COS were mediated by suppressing expression of proteins involved in H2O2‑induced apoptosis, including Bax, Bcl‑2 and cleaved PARP. Furthermore, the antagonist effects of NACOS were greater than those of COS, suggesting that acetylation was essential for the protective effects of COS.
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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Shi W and Huang Y: Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress. Exp Ther Med 28: 310, 2024.
APA
Li, Q., Shi, W., & Huang, Y. (2024). Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress. Experimental and Therapeutic Medicine, 28, 310. https://doi.org/10.3892/etm.2024.12600
MLA
Li, Q., Shi, W., Huang, Y."Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress". Experimental and Therapeutic Medicine 28.2 (2024): 310.
Chicago
Li, Q., Shi, W., Huang, Y."Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress". Experimental and Therapeutic Medicine 28, no. 2 (2024): 310. https://doi.org/10.3892/etm.2024.12600
Copy and paste a formatted citation
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Spandidos Publications style
Li Q, Shi W and Huang Y: Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress. Exp Ther Med 28: 310, 2024.
APA
Li, Q., Shi, W., & Huang, Y. (2024). Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress. Experimental and Therapeutic Medicine, 28, 310. https://doi.org/10.3892/etm.2024.12600
MLA
Li, Q., Shi, W., Huang, Y."Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress". Experimental and Therapeutic Medicine 28.2 (2024): 310.
Chicago
Li, Q., Shi, W., Huang, Y."Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress". Experimental and Therapeutic Medicine 28, no. 2 (2024): 310. https://doi.org/10.3892/etm.2024.12600
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