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Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease

  • Authors:
    • Mariana Cecilia Pérez‑Villalobos
    • Andrea Barba‑González
    • Nicté García‑Carrillo
    • Martín Humberto Muñoz‑Ortega
    • Esperanza Sánchez‑Alemán
    • Manuel Enrique Ávila‑Blanco
    • Jorge Christopher Morones‑Gamboa
    • Javier Ventura‑Juárez
    • Sandra Luz Martínez‑Hernández
  • View Affiliations / Copyright

    Affiliations: Department of Morphology, Center of Basic Sciences, Autonomous University of Aguascalientes, 20100 Aguascalientes, Mexico, Department of Chemistry, Center of Basic Sciences, Autonomous University of Aguascalientes, 20100 Aguascalientes, Mexico, Department of Microbiology, Center of Basic Sciences, Autonomous University of Aguascalientes, 20100 Aguascalientes, Mexico
    Copyright: © Pérez‑Villalobos et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 392
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    Published online on: August 7, 2024
       https://doi.org/10.3892/etm.2024.12681
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Abstract

Chronic kidney disease (CKD) is a progressive disease with a high mortality rate and a worldwide prevalence of 13.4%, triggered by various diseases with high incidence. The aim of the present study was to investigate the anti‑inflammatory and antifibrotic effect of pioglitazone on kidney in an adenine‑induced Wistar rats and the mechanisms possibly involved. CKD was induced in 40 rats. Rats were divided into two groups, which were split into the following sub‑groups: i) Therapeutic (pioglitazone administered after renal damage) divided into intact (healthy), adenine (CKD) and adenine/pioglitazone (treatment) and ii) prophylactic (adenine and pioglitazone administered at the same time) split into intact (healthy), adenine (CKD), endogenous reversion (recovery without treatment), adenine/pioglitazone (treatment) and pioglitazone sub‑groups. Reverse transcription‑quantitative PCR (collagen I, α‑SMA and TGF‑β), and hematoxylin‑eosin, Masson's trichrome and Sirius red staining were performed to measure histological markers of kidney damage, also the serum markers (urea, creatinine and uric acid) were performed, for analyze the effects of pioglitazone. In the adenine/pioglitazone rats of the therapeutic group, renal function parameters such as eGFR increased and serum creatinine decreased from those of untreated rats (CKD), however the renal index, serum urea, abnormalities in renal morphology, inflammatory cells and relative gene expression of collagen I, α‑SMA and TGF‑β did not change relative to the CKD rats. In adenine/pioglitazone rats, extracellular matrix collagen accumulation was significantly lower than the CKD rats. On the other hand, in adenine/pioglitazone rats of the prophylactic group, the renal index, creatinine, urea, uric acid serum and relative gene expression of collagen I, α‑SMA, and TGF‑β were significantly lower, as well as the presence of 2,8‑dihydroxyadenine crystals, and extracellular matrix collagen compared with CKD rats. In addition, the eGFR in the treatment group was similar to healthy rats, renal morphology was restored, and inflammatory cells were significantly lower. In conclusion, pioglitazone has a nephroprotective effect when administered in the early stages of kidney damage, reducing inflammatory and fibrotic processes and improving glomerular filtration rate. Furthermore, in the late phase of treatment, a tendency to decrease creatinine and increase eGFR was observed.
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Copy and paste a formatted citation
Spandidos Publications style
Pérez‑Villalobos MC, Barba‑González A, García‑Carrillo N, Muñoz‑Ortega MH, Sánchez‑Alemán E, Ávila‑Blanco ME, Morones‑Gamboa JC, Ventura‑Juárez J and Martínez‑Hernández SL: Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease. Exp Ther Med 28: 392, 2024.
APA
Pérez‑Villalobos, M.C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M.H., Sánchez‑Alemán, E., Ávila‑Blanco, M.E. ... Martínez‑Hernández, S.L. (2024). Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease. Experimental and Therapeutic Medicine, 28, 392. https://doi.org/10.3892/etm.2024.12681
MLA
Pérez‑Villalobos, M. C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M. H., Sánchez‑Alemán, E., Ávila‑Blanco, M. E., Morones‑Gamboa, J. C., Ventura‑Juárez, J., Martínez‑Hernández, S. L."Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease". Experimental and Therapeutic Medicine 28.4 (2024): 392.
Chicago
Pérez‑Villalobos, M. C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M. H., Sánchez‑Alemán, E., Ávila‑Blanco, M. E., Morones‑Gamboa, J. C., Ventura‑Juárez, J., Martínez‑Hernández, S. L."Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease". Experimental and Therapeutic Medicine 28, no. 4 (2024): 392. https://doi.org/10.3892/etm.2024.12681
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Spandidos Publications style
Pérez‑Villalobos MC, Barba‑González A, García‑Carrillo N, Muñoz‑Ortega MH, Sánchez‑Alemán E, Ávila‑Blanco ME, Morones‑Gamboa JC, Ventura‑Juárez J and Martínez‑Hernández SL: Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease. Exp Ther Med 28: 392, 2024.
APA
Pérez‑Villalobos, M.C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M.H., Sánchez‑Alemán, E., Ávila‑Blanco, M.E. ... Martínez‑Hernández, S.L. (2024). Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease. Experimental and Therapeutic Medicine, 28, 392. https://doi.org/10.3892/etm.2024.12681
MLA
Pérez‑Villalobos, M. C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M. H., Sánchez‑Alemán, E., Ávila‑Blanco, M. E., Morones‑Gamboa, J. C., Ventura‑Juárez, J., Martínez‑Hernández, S. L."Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease". Experimental and Therapeutic Medicine 28.4 (2024): 392.
Chicago
Pérez‑Villalobos, M. C., Barba‑González, A., García‑Carrillo, N., Muñoz‑Ortega, M. H., Sánchez‑Alemán, E., Ávila‑Blanco, M. E., Morones‑Gamboa, J. C., Ventura‑Juárez, J., Martínez‑Hernández, S. L."Nephroprotective effect of pioglitazone in a Wistar rat model of adenine‑induced chronic kidney disease". Experimental and Therapeutic Medicine 28, no. 4 (2024): 392. https://doi.org/10.3892/etm.2024.12681
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