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PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy

  • Authors:
    • Shuang Xia
    • Jin Li
    • Hongshan Yuan
    • Wenliang Yan
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu 210001, P.R. China, Department of Dermatology, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu 210001, P.R. China
    Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 402
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    Published online on: August 19, 2024
       https://doi.org/10.3892/etm.2024.12691
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Abstract

Peptidyl‑prolyl cis/trans isomerase, NIMA-interacting 1 (PIN1) has been suggested to be a critical regulator in skin‑related diseases. However, the role and molecular mechanism of PIN1 in psoriasis remain unclear. HaCaT cells were stimulated with five cytokines (M5) to induce psoriatic inflammation‑like conditions. Reverse transcription‑quantitative PCR and western blotting were performed to examine PIN1 expression in M5‑induced HaCaT cells. A Cell Counting Kit‑8 assay and 5‑ethynyl‑2'‑deoxyuridine staining were employed to examine cell proliferation. Inflammatory factors were evaluated using ELISA kits and western blot analysis. Mitochondrial autophagy was examined by immunofluorescence staining, western blotting and a JC‑1 assay. Western blot analysis was adopted to assess the levels of psoriasis marker proteins. PIN1 expression was markedly elevated in M5‑induced HaCaT cells. Silencing of PIN1 inhibited M5‑induced hyperproliferation and the inflammatory response, while it promoted mitochondrial autophagy in HaCaT cells. The addition of the mitochondrial autophagy inhibitor mitochondrial division inhibitor‑1 reversed the effects of PIN1 interference on proliferation, the inflammatory response and mitochondrial autophagy in M5‑induced HaCaT cells. The present study revealed that PIN1 inhibition protected HaCaT cells against M5‑induced hyperproliferation and inflammatory injury through the activation of mitochondrial autophagy.
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Copy and paste a formatted citation
Spandidos Publications style
Xia S, Li J, Yuan H and Yan W: PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy. Exp Ther Med 28: 402, 2024.
APA
Xia, S., Li, J., Yuan, H., & Yan, W. (2024). PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy. Experimental and Therapeutic Medicine, 28, 402. https://doi.org/10.3892/etm.2024.12691
MLA
Xia, S., Li, J., Yuan, H., Yan, W."PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy". Experimental and Therapeutic Medicine 28.4 (2024): 402.
Chicago
Xia, S., Li, J., Yuan, H., Yan, W."PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy". Experimental and Therapeutic Medicine 28, no. 4 (2024): 402. https://doi.org/10.3892/etm.2024.12691
Copy and paste a formatted citation
x
Spandidos Publications style
Xia S, Li J, Yuan H and Yan W: PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy. Exp Ther Med 28: 402, 2024.
APA
Xia, S., Li, J., Yuan, H., & Yan, W. (2024). PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy. Experimental and Therapeutic Medicine, 28, 402. https://doi.org/10.3892/etm.2024.12691
MLA
Xia, S., Li, J., Yuan, H., Yan, W."PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy". Experimental and Therapeutic Medicine 28.4 (2024): 402.
Chicago
Xia, S., Li, J., Yuan, H., Yan, W."PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy". Experimental and Therapeutic Medicine 28, no. 4 (2024): 402. https://doi.org/10.3892/etm.2024.12691
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