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Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4

  • Authors:
    • Hongbo Yu
    • Ji Qu
    • Haixin Gou
    • Ying Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine Traumatology and Surgery, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China, Department of Vascular Medicine, Shanghai Traditional Chinese Medicine‑Integrated Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200082, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 456
    |
    Published online on: October 15, 2024
       https://doi.org/10.3892/etm.2024.12746
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Abstract

Papillary thyroid carcinoma (PTC) is the most common malignant tumor of the endocrine system globally. Formononetin (FMNT), an isoflavonoid, exerts anti‑tumorigenic effects and chromobox homolog 4 (CBX4) exerts tumor‑promoting effect in specific types of tumors. Nevertheless, the predictive values and biological functions of FMNT and CBX4 in the pathological progress of PTC have not been fully understood till now. In the present study, the human PTC cell line TPC‑1 was exposed to 0, 10, 30 and 100 µM FMNT for 24 h to elucidate the precise effects of FMNT on the biological behaviors of PTC cells. Moreover, FMNT‑treated TPC‑1 cells were transfected with oe‑CBX4 to evaluate whether CBX4 was implicated in the anticarcinogenic effects of FMNT against PTC. It was demonstrated that FMNT treatment suppressed the proliferation, clone formation, migration, invasion, EMT, angiogenesis and stemness of PTC cells in a dose‑dependent manner. Furthermore, it was verified that FMNT targeted CBX4 to downregulate its expression in a dose dependent manner. The suppressive effects of FMNT on the proliferation, clone formation, migration, invasion, EMT, angiogenesis and stemness of PTC cells were partially reversed by CBX4 overexpression. Upregulation of CBX4 abolished the tumor suppression effects of FMNT in the malignant progression of PTC. In conclusion, FMNT might act as a promising anti‑tumorigenic agent in PTC, which depends on the downregulation of CBX4.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016.PubMed/NCBI View Article : Google Scholar

2 

Zhang W, Ruan X, Li Y, Zhi J, Hu L, Hou X, Shi X, Wang X, Wang J, Ma W, et al: KDM1A promotes thyroid cancer progression and maintains stemness through the Wnt/β-catenin signaling pathway. Theranostics. 12:1500–1517. 2022.PubMed/NCBI View Article : Google Scholar

3 

Haugen BR, Alexander EK, Bible KC, Doherty GM, Mandel SJ, Nikiforov YE, Pacini F, Randolph GW, Sawka AM, Schlumberger M, et al: 2015 American thyroid association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: The American thyroid association guidelines task force on thyroid nodules and differentiated thyroid cancer. Thyroid. 26:1–133. 2016.PubMed/NCBI View Article : Google Scholar

4 

Davies L and Welch HG: Current thyroid cancer trends in the United States. JAMA Otolaryngol Head Neck Surg. 140:317–322. 2014.PubMed/NCBI View Article : Google Scholar

5 

Jiang D, Rasul A, Batool R, Sarfraz I, Hussain G, Mateen Tahir M, Qin T, Selamoglu Z, Ali M, Li J and Li X: Potential anticancer properties and mechanisms of action of formononetin. Biomed Res Int. 2019(5854315)2019.PubMed/NCBI View Article : Google Scholar

6 

Xin M, Wang Y, Ren Q and Guo Y: Formononetin and metformin act synergistically to inhibit growth of MCF-7 breast cancer cells in vitro. Biomed Pharmacother. 109:2084–2089. 2019.PubMed/NCBI View Article : Google Scholar

7 

Chen J, Zeng J, Xin M, Huang W and Chen X: Formononetin induces cell cycle arrest of human breast cancer cells via IGF1/PI3K/Akt pathways in vitro and in vivo. Horm Metab Res. 43:681–686. 2011.PubMed/NCBI View Article : Google Scholar

8 

Zhou R, Xu L, Ye M, Liao M, Du H and Chen H: Formononetin inhibits migration and invasion of MDA-MB-231 and 4T1 breast cancer cells by suppressing MMP-2 and MMP-9 through PI3K/AKT signaling pathways. Horm Metab Res. 46:753–760. 2014.PubMed/NCBI View Article : Google Scholar

9 

Zhang L, Gong Y, Wang S and Gao F: Anti-colorectal cancer mechanisms of formononetin identified by network pharmacological approach. Med Sci Monit. 25:7709–7714. 2019.PubMed/NCBI View Article : Google Scholar

10 

Wang WS and Zhao CS: Formononetin exhibits anticancer activity in gastric carcinoma cell and regulating miR-542-5p. Kaohsiung J Med Sci. 37:215–225. 2021.PubMed/NCBI View Article : Google Scholar

11 

Yang C, Xie Q, Zeng X, Tao N, Xu Y, Chen Y, Wang J and Zhang L: Novel hybrids of podophyllotoxin and formononetin inhibit the growth, migration and invasion of lung cancer cells. Bioorg Chem. 85:445–454. 2019.PubMed/NCBI View Article : Google Scholar

12 

Chan HL and Morey L: Emerging Roles for polycomb-group proteins in stem cells and cancer. Trends Biochem Sci. 44:688–700. 2019.PubMed/NCBI View Article : Google Scholar

13 

Klauke K, Radulović V, Broekhuis M, Weersing E, Zwart E, Olthof S, Ritsema M, Bruggeman S, Wu X, Helin K, et al: Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation. Nat Cell Biol. 15:353–362. 2013.PubMed/NCBI View Article : Google Scholar

14 

Ismail IH, Gagné JP, Caron MC, McDonald D, Xu Z, Masson JY, Poirier GG and Hendzel MJ: CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res. 40:5497–5510. 2012.PubMed/NCBI View Article : Google Scholar

15 

Pan Y, Li Q, Cao Z and Zhao S: The SUMO E3 ligase CBX4 is identified as a poor prognostic marker of gastric cancer through multipronged OMIC analyses. Genes Dis. 8:827–837. 2021.PubMed/NCBI View Article : Google Scholar

16 

Wang B, Tang J, Liao D, Wang G, Zhang M, Sang Y, Cao J, Wu Y, Zhang R, Li S, et al: Chromobox homolog 4 is correlated with prognosis and tumor cell growth in hepatocellular carcinoma. Ann Surg Oncol. 20 (Suppl 3):S684–S692. 2013.PubMed/NCBI View Article : Google Scholar

17 

Hu C, Zhang Q, Tang Q, Zhou H, Liu W, Huang J, Liu Y, Wang Q, Zhang J, Zhou M, et al: CBX4 promotes the proliferation and metastasis via regulating BMI-1 in lung cancer. J Cell Mol Med. 24:618–631. 2020.PubMed/NCBI View Article : Google Scholar

18 

Fang X and Pan A: MiR-507 inhibits the progression of gastric carcinoma via targeting CBX4-mediated activation of Wnt/β-catenin and HIF-1α pathways. Clin Transl Oncol. 24:2021–2028. 2022.PubMed/NCBI View Article : Google Scholar

19 

Jiang N, Niu G, Pan YH, Pan W, Zhang MF, Zhang CZ and Shen H: CBX4 transcriptionally suppresses KLF6 via interaction with HDAC1 to exert oncogenic activities in clear cell renal cell carcinoma. EBioMedicine. 53(102692)2020.PubMed/NCBI View Article : Google Scholar

20 

Wen LJ, Wang YS and Tan PY: miR-515-5p inhibits the proliferation, migration and invasion of human breast cancer cells by targeting CBX4. Exp Ther Med. 22(1328)2021.PubMed/NCBI View Article : Google Scholar

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

22 

Winer A, Adams S and Mignatti P: Matrix metalloproteinase inhibitors in cancer therapy: Turning past failures into future successes. Mol Cancer Ther. 17:1147–1155. 2018.PubMed/NCBI View Article : Google Scholar

23 

Aiello NM and Kang Y: Context-dependent EMT programs in cancer metastasis. J Exp Med. 216:1016–1026. 2019.PubMed/NCBI View Article : Google Scholar

24 

Paduch R: The role of lymphangiogenesis and angiogenesis in tumor metastasis. Cell Oncol (Dordr). 39:397–410. 2016.PubMed/NCBI View Article : Google Scholar

25 

Khan AQ, Ahmed EI, Elareer N, Fathima H, Prabhu KS, Siveen KS, Kulinski M, Azizi F, Dermime S, Ahmad A, et al: Curcumin-mediated apoptotic cell death in papillary thyroid cancer and cancer stem-like cells through targeting of the JAK/STAT3 signaling pathway. Int J Mol Sci. 21(438)2020.PubMed/NCBI View Article : Google Scholar

26 

Li W, Chen H, Wang Z, Liu J, Lei X and Chen W: Chromobox 4 (CBX4) promotes tumor progression and stemness via activating CDC20 in gastric cancer. J Gastrointest Oncol. 13:1058–1072. 2022.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yu H, Qu J, Gou H and Zhou Y: Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4. Exp Ther Med 28: 456, 2024.
APA
Yu, H., Qu, J., Gou, H., & Zhou, Y. (2024). Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4. Experimental and Therapeutic Medicine, 28, 456. https://doi.org/10.3892/etm.2024.12746
MLA
Yu, H., Qu, J., Gou, H., Zhou, Y."Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4". Experimental and Therapeutic Medicine 28.6 (2024): 456.
Chicago
Yu, H., Qu, J., Gou, H., Zhou, Y."Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4". Experimental and Therapeutic Medicine 28, no. 6 (2024): 456. https://doi.org/10.3892/etm.2024.12746
Copy and paste a formatted citation
x
Spandidos Publications style
Yu H, Qu J, Gou H and Zhou Y: Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4. Exp Ther Med 28: 456, 2024.
APA
Yu, H., Qu, J., Gou, H., & Zhou, Y. (2024). Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4. Experimental and Therapeutic Medicine, 28, 456. https://doi.org/10.3892/etm.2024.12746
MLA
Yu, H., Qu, J., Gou, H., Zhou, Y."Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4". Experimental and Therapeutic Medicine 28.6 (2024): 456.
Chicago
Yu, H., Qu, J., Gou, H., Zhou, Y."Formononetin suppresses the malignant progression of papillary thyroid carcinoma depending on downregulation of CBX4". Experimental and Therapeutic Medicine 28, no. 6 (2024): 456. https://doi.org/10.3892/etm.2024.12746
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