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Magnolol acts as a neurorestorative agent in an Aβ1‑42‑induced mouse model of Alzheimer's disease

  • Authors:
    • Qian Yu
    • Yuchen Shi
    • Yuehong Wu
    • Ruifei Liu
    • Hui Zhang
    • Lingling Wu
    • Mingxing Ding
  • View Affiliations / Copyright

    Affiliations: Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, Jinhua, Zhejiang 321017, P.R. China, Department of Psychiatry, Jinhua Second Hospital, Jinhua, Zhejiang 321016, P.R. China, Jinhua Institute for Food and Drug Control, Institute of Pharmacology, Jinhua, Zhejiang 321000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 12
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    Published online on: November 14, 2024
       https://doi.org/10.3892/etm.2024.12762
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Abstract

Magnolol may have the potential to alleviate the progression of Alzheimer's disease (AD). The present study was conducted to investigate the broader mechanism of action of magnolol in AD pathogenesis. C57BL/6 mice were randomly divided into five groups (n=6 mice/group): i) Control; ii) AD model; iii) 5 mg/kg magnolol + AD model; iv) 10 mg/kg magnolol + AD model; and v) 20 mg/kg magnolol + AD model. A total of 7 days after modeling, the treatment groups were administered different doses of magnolol (5, 10 and 20 mg/kg) by gavage every day, and a Morris water maze test was conducted after 2 months of treatment. The impacts of magnolol on amyloid β (Aβ) plaque deposition and neuroinflammation were assessed using Congo red and immunofluorescence staining. Immunofluorescence staining results were supplemented with western blotting and reverse transcription‑quantitative PCR to ascertain the role of magnolol in other pivotal pathological mechanisms, including the formation of intracellular neurofibrillary tangles, compromised synaptic plasticity, and astrocyte and microglia activation. Administration of magnolol effectively mitigated cognitive impairment, reduced Aβ plaque deposition and inhibited neuroinflammation in Aβ1‑42‑induced mice. Moreover, hippocampal levels of tau, phosphorylated (p‑)tau, glycogen synthase kinase 3β (GSK3β), p‑GSK3β, synaptophysin, brain‑derived neurotrophic factor, glial fibrillary acidic protein and ionized calcium binding adaptor molecule 1 revealed that magnolol also limited neurofibrillary tangle formation, repaired synaptic plasticity, and inhibited astrocyte and microglia activation. In conclusion, the present findings broaden the current understanding of the mechanisms explaining the neuroprotective effects of magnolol against AD progression. Notably, it may inhibit multiple manifestations of AD, including plaques and neuroinflammation, while also exhibiting the capacity to restore AD‑related neurological damage.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Q, Shi Y, Wu Y, Liu R, Zhang H, Wu L and Ding M: Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease. Exp Ther Med 29: 12, 2025.
APA
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., & Ding, M. (2025). Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease. Experimental and Therapeutic Medicine, 29, 12. https://doi.org/10.3892/etm.2024.12762
MLA
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., Ding, M."Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease". Experimental and Therapeutic Medicine 29.1 (2025): 12.
Chicago
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., Ding, M."Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease". Experimental and Therapeutic Medicine 29, no. 1 (2025): 12. https://doi.org/10.3892/etm.2024.12762
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Q, Shi Y, Wu Y, Liu R, Zhang H, Wu L and Ding M: Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease. Exp Ther Med 29: 12, 2025.
APA
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., & Ding, M. (2025). Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease. Experimental and Therapeutic Medicine, 29, 12. https://doi.org/10.3892/etm.2024.12762
MLA
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., Ding, M."Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease". Experimental and Therapeutic Medicine 29.1 (2025): 12.
Chicago
Yu, Q., Shi, Y., Wu, Y., Liu, R., Zhang, H., Wu, L., Ding, M."Magnolol acts as a neurorestorative agent in an A<sub>&beta;1‑42</sub>‑induced mouse model of Alzheimer's disease". Experimental and Therapeutic Medicine 29, no. 1 (2025): 12. https://doi.org/10.3892/etm.2024.12762
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