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Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva

  • Authors:
    • Daiki Fujii
    • Hiroshi Nango
    • Masahiro Ohtani
  • View Affiliations / Copyright

    Affiliations: Central Research Institute, Wakunaga Pharmaceutical Co., Ltd., Akitakata, Hiroshima 739‑1195, Japan
    Copyright: © Fujii et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 41
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    Published online on: December 30, 2024
       https://doi.org/10.3892/etm.2024.12791
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Abstract

Periodontal disease is recognized as a chronic multifactorial inflammatory condition initiated by dysbiosis within subgingival plaque biofilms. Antimicrobial peptides exhibit a wide spectrum of antimicrobial action, and thus, provide one of the first lines of host defense against oral pathogens. Aged garlic extract (AGE) is effective for preventing the progression of periodontal disease. The present study examined whether AGE affects the production of antimicrobial peptides in mouse gingiva. Reverse transcription‑quantitative PCR analysis demonstrated that oral administration of AGE in mice increased the mRNA level of Defb4 in gingival tissue, while the levels of Defb1, Defb14 and Cramp remained unaffected. AGE also upregulated the protein levels of β‑defensin 4. To explore the underlying mechanism of the increased β‑defensin 4 production induced by AGE, a comprehensive phosphoproteomic analysis in gingival tissues was performed. Proteomic profiling revealed activation of the canonical Wnt/β‑catenin pathway in gingiva of mice treated with AGE. Treatment of mouse gingival epithelial GE1 cells with AGE resulted in an increase of β‑defensin 4 in the culture medium. In support of proteomics experiments, LF3, a specific inhibitor of Wnt/β‑catenin signaling, suppressed the AGE‑induced production of β‑defensin 4. In addition, β‑catenin protein was found to accumulate within the nucleus in cells treated with AGE. In conclusion, the present findings suggested that AGE enhanced the production of β‑defensin 4 in mouse gingiva through the canonical Wnt signal transduction pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fujii D, Nango H and Ohtani M: Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva. Exp Ther Med 29: 41, 2025.
APA
Fujii, D., Nango, H., & Ohtani, M. (2025). Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva. Experimental and Therapeutic Medicine, 29, 41. https://doi.org/10.3892/etm.2024.12791
MLA
Fujii, D., Nango, H., Ohtani, M."Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva". Experimental and Therapeutic Medicine 29.2 (2025): 41.
Chicago
Fujii, D., Nango, H., Ohtani, M."Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva". Experimental and Therapeutic Medicine 29, no. 2 (2025): 41. https://doi.org/10.3892/etm.2024.12791
Copy and paste a formatted citation
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Spandidos Publications style
Fujii D, Nango H and Ohtani M: Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva. Exp Ther Med 29: 41, 2025.
APA
Fujii, D., Nango, H., & Ohtani, M. (2025). Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva. Experimental and Therapeutic Medicine, 29, 41. https://doi.org/10.3892/etm.2024.12791
MLA
Fujii, D., Nango, H., Ohtani, M."Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva". Experimental and Therapeutic Medicine 29.2 (2025): 41.
Chicago
Fujii, D., Nango, H., Ohtani, M."Aged garlic extract enhances the production of β‑defensin 4 via activation of the Wnt/β‑catenin pathway in mouse gingiva". Experimental and Therapeutic Medicine 29, no. 2 (2025): 41. https://doi.org/10.3892/etm.2024.12791
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