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Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia

  • Authors:
    • Nan Lin
    • Liping Lin
    • Xinxiang Huang
    • Chaozhong Huang
    • Jinrong Gong
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 133
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    Published online on: May 13, 2025
       https://doi.org/10.3892/etm.2025.12883
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Abstract

Early detection and diagnosis are key to improving the survival rate and reducing the fatality rate linked to gastric cancer. The precancerous lesion of gastric cancer is referred to as gastric high‑grade intraepithelial neoplasia (HGIN). Both the sensitivity and specificity of current biomarkers that aid in the diagnosis of gastric HGIN are still relatively low. Furthermore, proteomic data on gastric HGIN are still scarce. The present study aimed to explore candidate protein biomarkers for gastric HGIN screening with proteomics and bioinformatics technology. A total of 10 serum samples were collected and categorized into two groups, i.e., the gastric HGIN and the healthy control groups. Label‑free quantification in conjunction with liquid chromatography with tandem mass spectrometry was employed to identify the probable biomarkers for gastric HGIN. Furthermore, differentially expressed proteins (DEPs) were quantified by proteomics analysis. In total, 1,192 distinct serum proteins were discovered between the gastric HGIN group and the healthy control group. DEPs were identified in the further analyses, utilizing a threshold of a 1.5‑fold difference in expression level (P<0.05) in comparison with the control group. There were 18 upregulated and 12 downregulated proteins in the gastric HGIN group in comparison with the control group. Bioinformatics analyses were performed using Gene Ontology and KEGG pathway enrichment analyses. The GO analysis revealed that the DEPs were enriched in biological processes such as ‘cellular’, ‘biological regulation’, ‘multicellular organismal’, ‘developmental’ and ‘reaction to stimulus processes’, localized to ‘cell’, ‘intracellular’ and ‘protein‑containing complex’, and involved in molecular functions such as ‘molecular function modulator’, ‘binding’ and ‘catalytic activity’. The KEGG pathway enrichment analysis manifested that the DEPs were predominantly enriched in ‘antigen processing and presentation’, ‘diabetic cardiomyopathy’, ‘Epstein‑Barr virus infection’, ‘herpes simplex virus 1 infection’, ‘human immunodeficiency virus 1 infection’ and ‘human cytomegalovirus infection’. In conclusion, the present data provide more biological information for the formation of gastric HGIN and clues for further research on the pathogenesis of early gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lin N, Lin L, Huang X, Huang C and Gong J: Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia. Exp Ther Med 30: 133, 2025.
APA
Lin, N., Lin, L., Huang, X., Huang, C., & Gong, J. (2025). Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia. Experimental and Therapeutic Medicine, 30, 133. https://doi.org/10.3892/etm.2025.12883
MLA
Lin, N., Lin, L., Huang, X., Huang, C., Gong, J."Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 30.1 (2025): 133.
Chicago
Lin, N., Lin, L., Huang, X., Huang, C., Gong, J."Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 30, no. 1 (2025): 133. https://doi.org/10.3892/etm.2025.12883
Copy and paste a formatted citation
x
Spandidos Publications style
Lin N, Lin L, Huang X, Huang C and Gong J: Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia. Exp Ther Med 30: 133, 2025.
APA
Lin, N., Lin, L., Huang, X., Huang, C., & Gong, J. (2025). Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia. Experimental and Therapeutic Medicine, 30, 133. https://doi.org/10.3892/etm.2025.12883
MLA
Lin, N., Lin, L., Huang, X., Huang, C., Gong, J."Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 30.1 (2025): 133.
Chicago
Lin, N., Lin, L., Huang, X., Huang, C., Gong, J."Label‑free quantitative proteomics of gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 30, no. 1 (2025): 133. https://doi.org/10.3892/etm.2025.12883
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