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Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis

  • Authors:
    • Chun-Chao Chang
    • Chih-Ju Chang
    • Chih-Yi Liu
    • Hsing-Jung Yeh
    • Yu-Ju Li
    • Wen-Chao Chen
    • Chun-Wei Chang
    • Jun-Liang Pan
    • Yu-Chuan Liu
    • Chi-Jung Huang
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Division of Gastroenterology and Hepatology, Taipei Medical University Hospital, Taipei 110301, Taiwan, R.O.C., Department of Surgery, Division of Neurosurgery, Cathay General Hospital, Taipei 106438, Taiwan, R.O.C., School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242062, Taiwan, R.O.C., Department of Internal Medicine, Division of Cardiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807378, Taiwan, R.O.C., Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan, R.O.C., Department of Biochemistry, National Defense Medical Center, Taipei 114201, Taiwan, R.O.C.
    Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 146
    |
    Published online on: May 27, 2025
       https://doi.org/10.3892/etm.2025.12896
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Abstract

Ulcerative colitis (UC) is a chronic, relapsing inflammatory bowel disease characterized by disruption of the intestinal epithelial barrier and alterations in mucosal gene expression associated with intestinal integrity. Given the risks associated with UC, novel therapies capable of restoring intestinal barrier function and inhibiting inflammation are needed. Plasmon‑activated water (PAW) is a nontoxic form of water with potential in the treatment of inflammatory diseases. The aim of the present study was to evaluate the therapeutic effects of PAW in a murine model of UC. Histological and immunohistochemical analyses were performed on colon tissues from mice with dextran sodium sulfate (DSS)‑induced UC treated with either 5‑aminosalicylic acid (5‑ASA) or PAW. Epithelial cell density was decreased in the DSS model mice compared with that in the normal control mice, whereas treatment with 5‑ASA or PAW attenuated this DSS‑induced reduction. Microscopy revealed that the DSS/PAW group exhibited significantly reduced epithelial loss, crypt damage and inflammatory cell infiltration compared with that in the DSS group. In addition, immunohistochemical analysis demonstrated that PAW downregulated the DSS‑induced expression of tumor necrosis factor‑α and keratin 20 in epithelial cells and the lamina propria. Furthermore, PAW also attenuated the DSS‑induced loss of expression of three proteins essential for cell adhesion and tight junctions, namely E‑cadherin (CDH1), tight junction protein 1 (ZO‑1) and occludin, in the colonic epithelium, particularly in intestinal crypts. In addition, mucin 1 (MUC1) expression was decreased and MUC2 expression increased in the mucosal layer of the colons of the DSS/PAW group compared with those in the DSS group. In conclusion, the colonic mucosa is a reliable site for evaluating epithelial damage and inflammatory infiltration. PAW ameliorated DSS‑induced UC in mice by modulating the expression of key barrier‑associated proteins, including CDH1, occludin, ZO‑1, MUC1 and MUC2. These findings highlight the therapeutic potential of PAW in the treatment of colitis.
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Copy and paste a formatted citation
Spandidos Publications style
Chang C, Chang C, Liu C, Yeh H, Li Y, Chen W, Chang C, Pan J, Liu Y, Huang C, Huang C, et al: Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis. Exp Ther Med 30: 146, 2025.
APA
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W. ... Huang, C. (2025). Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis. Experimental and Therapeutic Medicine, 30, 146. https://doi.org/10.3892/etm.2025.12896
MLA
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W., Chang, C., Pan, J., Liu, Y., Huang, C."Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis". Experimental and Therapeutic Medicine 30.1 (2025): 146.
Chicago
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W., Chang, C., Pan, J., Liu, Y., Huang, C."Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis". Experimental and Therapeutic Medicine 30, no. 1 (2025): 146. https://doi.org/10.3892/etm.2025.12896
Copy and paste a formatted citation
x
Spandidos Publications style
Chang C, Chang C, Liu C, Yeh H, Li Y, Chen W, Chang C, Pan J, Liu Y, Huang C, Huang C, et al: Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis. Exp Ther Med 30: 146, 2025.
APA
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W. ... Huang, C. (2025). Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis. Experimental and Therapeutic Medicine, 30, 146. https://doi.org/10.3892/etm.2025.12896
MLA
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W., Chang, C., Pan, J., Liu, Y., Huang, C."Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis". Experimental and Therapeutic Medicine 30.1 (2025): 146.
Chicago
Chang, C., Chang, C., Liu, C., Yeh, H., Li, Y., Chen, W., Chang, C., Pan, J., Liu, Y., Huang, C."Plasmon‑activated water enhances gut‑barrier function and alleviates inflammation in a mouse model of ulcerative colitis". Experimental and Therapeutic Medicine 30, no. 1 (2025): 146. https://doi.org/10.3892/etm.2025.12896
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