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Article

Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate

  • Authors:
    • Ramesh Ummanni
    • Ulrike Lehnigk
    • Uwe Zimmermann
    • Christian Woenckhaus
    • Reinhard Walther
    • Jürgen Giebel
  • View Affiliations / Copyright

    Affiliations: Department of Medical Biochemistry and Molecular Biology, Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany
  • Pages: 47-52
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/etm_00000008
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Abstract

Activation of caspases is an essential prerequisite for induction of apoptosis. In many tumors caspases are down-regulated, while anti-apoptotic Bcl-2 is up-regulated. To elucidate their putative role in prostate cancer (PCa) we determined the expression of different caspases and Bcl-2 in benign prostate epithelium (BPE) and PCa. Paraffin-embedded prostate whole mounts were cut (4 µm) and investigated immunohistochemically using monoclonal antibodies against caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6, and Bcl-2. In BPE all caspases were localized to the cytoplasm of glandular cells. In PCa we found a statistically significant reduction in cleaved caspase-3 and -6 compared to the levels in BPE. The Bcl-2 protein was detected in the basal compartment of epithelial gland cells, but no immunostaining was noted in PCa. The decreased immunoreactivity of activated caspases probably indicates an alteration in post-translational cleavage that may play an important role during PCa progression.
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Copy and paste a formatted citation
Spandidos Publications style
Ummanni R, Lehnigk U, Zimmermann U, Woenckhaus C, Walther R and Giebel J: Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate . Exp Ther Med 1: 47-52, 2010.
APA
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., & Giebel, J. (2010). Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate . Experimental and Therapeutic Medicine, 1, 47-52. https://doi.org/10.3892/etm_00000008
MLA
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., Giebel, J."Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate ". Experimental and Therapeutic Medicine 1.1 (2010): 47-52.
Chicago
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., Giebel, J."Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 47-52. https://doi.org/10.3892/etm_00000008
Copy and paste a formatted citation
x
Spandidos Publications style
Ummanni R, Lehnigk U, Zimmermann U, Woenckhaus C, Walther R and Giebel J: Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate . Exp Ther Med 1: 47-52, 2010.
APA
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., & Giebel, J. (2010). Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate . Experimental and Therapeutic Medicine, 1, 47-52. https://doi.org/10.3892/etm_00000008
MLA
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., Giebel, J."Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate ". Experimental and Therapeutic Medicine 1.1 (2010): 47-52.
Chicago
Ummanni, R., Lehnigk, U., Zimmermann, U., Woenckhaus, C., Walther, R., Giebel, J."Immunohistochemical expression of caspase-1 and -9, uncleaved caspase-3 and -6, cleaved caspase-3 and -6 as well as Bcl-2 in benign epithelium and cancer of the prostate ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 47-52. https://doi.org/10.3892/etm_00000008
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