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Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test

  • Authors:
    • Miki Tanioka
    • Atsushi Utani
    • Eri Araki
    • Hiroko Fujii
    • Satoshi Kore-Eda
    • Takao Tachibana
    • Toshikazu Takano
    • Hisayuki Kobayashi
    • Haruhiko Nakajima
    • Yoshiki Miyachi
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. mtanioka@fukui-med.jrc.or.jp
  • Pages: 65-68
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/etm_00000011
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Abstract

Malignant melanoma usually shows resistance to a standard chemotherapy regimen. A useful in vitro method to evaluate individual chemosensitivity is required to select effective anti-cancer drugs. This study aimed to establish in vitro tumor response testing for malignant melanoma. We determined the chemosensitivity of primary cultured melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test (CD-DST). Nineteen tests were carried out for 15 cases of malignant melanoma. Primary cultured melanoma cells in collagen gel droplets were exposed to anti-cancer drugs, including cisplatin, adriamycin, dacarbazine, nimustine and vincristine. After a 7-day incubation in a serum-free medium, living melanoma cells in a collagen droplet were detected by image analysis after staining with Neutral red reagent. In vitro drug exposure conditions were determined to reproduce the value of the plasma area under the time-drug concentration curve in vivo. The rate of evaluation of the primary culture of melanoma cells was 78.9% (15/19 tests). The chemosensitivity of cisplatin, adriamycin, dacarbazine, nimustine and vincristine was 15, 62, 0, 0 and 62%, respectively. Dacarbazine was not suitable for CD-DST due to its prodrug characteristics. The CD-DST method was able to evaluate the chemosensitivity of malignant melanoma to anti-cancer drugs in vitro. This method can also be applied to estimate the efficacy of newly developed anti-cancer drugs in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Tanioka M, Utani A, Araki E, Fujii H, Kore-Eda S, Tachibana T, Takano T, Kobayashi H, Nakajima H, Miyachi Y, Miyachi Y, et al: Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test . Exp Ther Med 1: 65-68, 2010.
APA
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T. ... Miyachi, Y. (2010). Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test . Experimental and Therapeutic Medicine, 1, 65-68. https://doi.org/10.3892/etm_00000011
MLA
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T., Takano, T., Kobayashi, H., Nakajima, H., Miyachi, Y."Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test ". Experimental and Therapeutic Medicine 1.1 (2010): 65-68.
Chicago
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T., Takano, T., Kobayashi, H., Nakajima, H., Miyachi, Y."Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 65-68. https://doi.org/10.3892/etm_00000011
Copy and paste a formatted citation
x
Spandidos Publications style
Tanioka M, Utani A, Araki E, Fujii H, Kore-Eda S, Tachibana T, Takano T, Kobayashi H, Nakajima H, Miyachi Y, Miyachi Y, et al: Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test . Exp Ther Med 1: 65-68, 2010.
APA
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T. ... Miyachi, Y. (2010). Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test . Experimental and Therapeutic Medicine, 1, 65-68. https://doi.org/10.3892/etm_00000011
MLA
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T., Takano, T., Kobayashi, H., Nakajima, H., Miyachi, Y."Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test ". Experimental and Therapeutic Medicine 1.1 (2010): 65-68.
Chicago
Tanioka, M., Utani, A., Araki, E., Fujii, H., Kore-Eda, S., Tachibana, T., Takano, T., Kobayashi, H., Nakajima, H., Miyachi, Y."Evaluation of the chemosensitivity of primary cultured malignant melanoma cells using the collagen gel droplet-embedded culture drug sensitivity test ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 65-68. https://doi.org/10.3892/etm_00000011
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