Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
January-February 2010 Volume 1 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-February 2010 Volume 1 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging

  • Authors:
    • João A.R. Brito
    • Carolina C. Gomes
    • Flávio J.G.S. Pimenta
    • Alvimar A. Barbosa
    • Marco A.M. Prado
    • Vânia F. Prado
    • Marcus V. Gomez
    • Ricardo S. Gomez
  • View Affiliations / Copyright

    Affiliations: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
  • Pages: 217-221
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/etm_00000035
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRNAs) mir15a and let7a are important regulators of bcl-2, ras and c-myc proteins. Considering that these miRNAs are commonly altered in many human cancers and that these proteins are reported to be altered in oral squamous cell carcinoma (OSCC), we investigated them in a set of OSCC cases. The miRNAs as well as the proteins were evaluated in the tumor and blood of 20 patients by real-time quantitative PCR and immunohistochemistry, respectively. The expression of mir15a and bcl-2 proteins in the tumors was not associated with each other or with tumor staging. On the other hand, we found reduced expression of this miRNA in the blood of patients with an advanced stage of OSCC and with lymph node metastasis. The expression of let7a in the tumor and blood was not associated with tumor size, lymph node metastasis, tumor staging and immunoexpression of ras and c-myc proteins. In conclusion, the present study shows that reduced expression of mir15a is associated with OSCC staging.
View Figures

Figure 1

Figure 2

View References

1. 

Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI

2. 

Zhang B, Pan X, Cobb GP and Anderson TA: microRNAs as oncogenes and tumor suppressors. Dev Biol. 302:1–12. 2007. View Article : Google Scholar : PubMed/NCBI

3. 

Huppi K, Volfovsky N, Mackiewicz M, et al: MicroRNAs and genomic instability. Semin Cancer Biol. 17:65–73. 2007. View Article : Google Scholar : PubMed/NCBI

4. 

Kent OA and Mendell JT: A small piece in the cancer puzzle: microRNAs as tumor suppressors and oncogenes. Oncogene. 25:6188–6196. 2006. View Article : Google Scholar : PubMed/NCBI

5. 

Kumar MS, Lu J, Mercer KL, Golub TR and Jacks T: Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nat Genet. 39:673–677. 2007. View Article : Google Scholar : PubMed/NCBI

6. 

Warnasulasuriya S: Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 45:309–316. 2009. View Article : Google Scholar

7. 

Gomes CC and Gomez RS: MicroRNA and oral cancer. Oral Oncol. 44:910–914. 2008. View Article : Google Scholar : PubMed/NCBI

8. 

Barnes L, Everson JW, Reichart P and Sidransky D: WHO - World Health Organization Classification of Tumours, Pathology and Genetics of Head and Neck Tumours. IARC Press; Lyon: pp. 4332005

9. 

Chen C, Ridzon DA, Broomer AJ, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:1–9. 2005. View Article : Google Scholar : PubMed/NCBI

10. 

Livak KJ and Schimittgen T: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001.

11. 

Tran N, McLean T, Zhang X, et al: MicroRNA expression profiles in head and neck cancer cell lines. Biochem Biophys Res Commun. 358:12–17. 2007. View Article : Google Scholar

12. 

Jiang J, Lee EJ, Gusev Y and Schimittgen TD: Real-time expression profiling of microRNA precursors in human cancer cell lines. Nucleic Acids Res. 33:5394–5403. 2005. View Article : Google Scholar : PubMed/NCBI

13. 

Gaur A, Jewell DA, Liang Y, et al: Characterization of microRNA expression levels and their biological correlates in human cancer cell lines. Cancer Res. 67:2456–2468. 2007. View Article : Google Scholar : PubMed/NCBI

14. 

Calin GA, Dumitru CD, Shimizu M, et al: Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci USA. 99:15524–15529. 2002. View Article : Google Scholar : PubMed/NCBI

15. 

Cimmino A, Calin GA, Fabbri M, et al: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA. 102:13944–13949. 2005. View Article : Google Scholar : PubMed/NCBI

16. 

Camisasca DR, Honorato J, Bernardo V, et al: Expression of Bcl-2 family proteins and associated clinicopathologic factors predict survival outcome in patients with oral squamous cell carcinoma. Oral Oncol. 45:225–233. 2009. View Article : Google Scholar : PubMed/NCBI

17. 

Ohigashi T, Ueno N, Novaka S, et al: Tyrosine kinase inhibitors reduce bcl-2 expression and induce apoptosis in androgen-dependent cells. Am J Physiol Cell Physiol. 278:C66–C72. 2000.PubMed/NCBI

18. 

Ogawara K, Miyakawa A, Shiba M, et al: Allelic loss of chromosome 13q14.3 in human oral cancer correlation with lymph node metastasis. Int J Cancer. 79:312–317. 1998. View Article : Google Scholar : PubMed/NCBI

19. 

Takamizawa J, Konishi H, Yanagizawa K, et al: Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 64:3753–3756. 2004. View Article : Google Scholar : PubMed/NCBI

20. 

Akao Y, Nakagawa Y and Naoe T: Let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull. 29:903–906. 2006. View Article : Google Scholar : PubMed/NCBI

21. 

Childs G, Fazzari M, Kung G, et al: Low-level expression of microRNAs let-7d and mir-205 are prognostic markers of head and neck squamous cell carcinoma. Am J Pathol. 174:736–745. 2009. View Article : Google Scholar : PubMed/NCBI

22. 

Chang KW, Liu CJ, Chu TH, et al: Association between high mir-211 microRNA Expression and the poor prognosis of oral carcinoma. J Dent Res. 87:1063–1068. 2008. View Article : Google Scholar : PubMed/NCBI

23. 

Johnson SM, Grosshans H, Shingara J, et al: RAS is regulated by the let-7 microRNA family. Cell. 120:635–647. 2005. View Article : Google Scholar : PubMed/NCBI

24. 

Christensen BC, Moyer BJ, Avissar M, et al: Let-7 microRNA binging site polymorphism in the KRAS 3′UTR is associated with reduced survival in oral cancers. Carcinogenesis. 30:1003–1007. 2009.

25. 

Perez-Ordoñez B, Beauchemin M and Jordan RCK: Molecular biology of squamous cell carcinoma of the head and neck. J Clin Pathol. 59:445–453. 2006.

26. 

Hardisson D: Molecular pathogenesis of head and neck squamous cell carcinoma. Head Neck Oncol. 260:502–508. 2003.PubMed/NCBI

27. 

Das BR and Nagpal JK: Understanding the biology of oral cancer. Med Sci Monit. 8:258–267. 2002.

28. 

Fukasawa K: Centrosome amplification, chromosome instability and cancer development. Cancer Lett. 230:6–19. 2005. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Brito JA, Gomes CC, Pimenta FJ, Barbosa AA, Prado MA, Prado VF, Gomez MV and Gomez RS: Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging . Exp Ther Med 1: 217-221, 2010.
APA
Brito, J.A., Gomes, C.C., Pimenta, F.J., Barbosa, A.A., Prado, M.A., Prado, V.F. ... Gomez, R.S. (2010). Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging . Experimental and Therapeutic Medicine, 1, 217-221. https://doi.org/10.3892/etm_00000035
MLA
Brito, J. A., Gomes, C. C., Pimenta, F. J., Barbosa, A. A., Prado, M. A., Prado, V. F., Gomez, M. V., Gomez, R. S."Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging ". Experimental and Therapeutic Medicine 1.1 (2010): 217-221.
Chicago
Brito, J. A., Gomes, C. C., Pimenta, F. J., Barbosa, A. A., Prado, M. A., Prado, V. F., Gomez, M. V., Gomez, R. S."Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 217-221. https://doi.org/10.3892/etm_00000035
Copy and paste a formatted citation
x
Spandidos Publications style
Brito JA, Gomes CC, Pimenta FJ, Barbosa AA, Prado MA, Prado VF, Gomez MV and Gomez RS: Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging . Exp Ther Med 1: 217-221, 2010.
APA
Brito, J.A., Gomes, C.C., Pimenta, F.J., Barbosa, A.A., Prado, M.A., Prado, V.F. ... Gomez, R.S. (2010). Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging . Experimental and Therapeutic Medicine, 1, 217-221. https://doi.org/10.3892/etm_00000035
MLA
Brito, J. A., Gomes, C. C., Pimenta, F. J., Barbosa, A. A., Prado, M. A., Prado, V. F., Gomez, M. V., Gomez, R. S."Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging ". Experimental and Therapeutic Medicine 1.1 (2010): 217-221.
Chicago
Brito, J. A., Gomes, C. C., Pimenta, F. J., Barbosa, A. A., Prado, M. A., Prado, V. F., Gomez, M. V., Gomez, R. S."Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 217-221. https://doi.org/10.3892/etm_00000035
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team