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Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer

  • Authors:
    • Sunkoji Srujan
    • Suchanda Sahu
    • Tushar Subhadarshan Mishra
    • Dilip Kumar Muduly
    • Pritinanda Mishra
    • Sudipta Mohakud
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India, Department of General Surgery, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India, Department of Surgical Oncology, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India, Department of Pathology, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India, Department of Radio‑diagnosis, All India Institute of Medical Sciences, Bhubaneswar, Odisha 751019, India
    Copyright: © Srujan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 5
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    Published online on: June 24, 2025
       https://doi.org/10.3892/ije.2025.28
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Abstract

Circulating microRNAs (miRNAs/miRs) are found in numerous body fluids in healthy and disease conditions. In various types of malignancies, miRNAs are abnormally expressed and dysregulated. The present study determined and compared the circulating levels of miR‑27a, ‑122 and ‑155 in patients with early‑stage breast cancer, benign breast tumors and healthy controls. For this purpose, samples were collected from 56 newly‑diagnosed patients with early‑stage breast cancer, 56 patients with benign breast tumors and 56 age‑matched healthy controls. Serum was separated and reverse transcription‑quantitative PCR was used to quantify the expression levels of miRNAs. The diagnostic efficacy of individual miRNAs and the combined effect of all three miRNAs were compared in the three groups. The results revealed that the expression levels of the three miRNAs (miR‑27a, miR‑122 and miR‑155) in serum were significantly increased in patients with early‑stage breast cancer when compared to those with benign breast tumors patients and the healthy controls (P<0.001). Receiver‑operating‑characteristic curve (ROC) analysis indicated that the serum levels of the miRNAs, miR‑27a, miR‑122 and miR‑155, exhibited sensitivity (80.4, 78.6 and 69.6%, respectively) and specificity (89.3, 98.2 and 71.4%, respectively) for the detection of early‑stage breast cancer in patients compared to healthy controls. ROC curve analysis also revealed that the serum of the three miRNAs exhibited sensitivity (76.8, 78.6 and 69.6%%, respectively) and specificity (98.2, 87.5 and 78.6%, respectively) for the detection of early‑stage breast cancer in patients compared to benign breast tumors. On the whole, the present study demonstrates a considerable diagnostic accuracy of these miRNAs for the detection of early‑stage breast cancer. These miRNAs thus have potential for use as non‑invasive biomarkers for the diagnosis and screening of breast cancer.
View Figures

Figure 1

Amplification plot representing
microRNAs-16, -27a, -122, -155 gene expression.

Figure 2

miR-27a fold change values in patients
with early-stage breast cancer, benign breast tumors and healthy
controls. The circles on top of the box plots denote the outliers.
FC, fold change; BC, breast cancer; HC, healthy controls.

Figure 3

miR-122 fold change values in patients
with early-stage breast cancer, benign breast tumors and healthy
controls. The circles on top of the box plots denote the outliers.
FC, fold change; BC, breast cancer; HC, healthy controls.

Figure 4

miR-155 fold change values in patients
with early-stage breast cancer, benign breast tumors and healthy
controls. The circles on top of the box plots denote the outliers.
*P<0-05. FC, fold change; BC, breast cancer; HC,
healthy controls.

Figure 5

Receiver operating characteristic
curve analysis of the three miRNAs for differentiating between
patients with early-stage breast cancer and healthy controls.

Figure 6

Receiver operating characteristic
curve analysis of the three miRNAs for differentiating between
patients with early-stage breast cancer and those with benign
breast tumors.

Figure 7

Receiver operating characteristic
curve analysis of the three miRNAs for differentiating between
patients with benign breast tumors and healthy controls.
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Copy and paste a formatted citation
Spandidos Publications style
Srujan S, Sahu S, Mishra TS, Muduly DK, Mishra P and Mohakud S: Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer. Int J Epigen 5: 5, 2025.
APA
Srujan, S., Sahu, S., Mishra, T.S., Muduly, D.K., Mishra, P., & Mohakud, S. (2025). Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer. International Journal of Epigenetics, 5, 5. https://doi.org/10.3892/ije.2025.28
MLA
Srujan, S., Sahu, S., Mishra, T. S., Muduly, D. K., Mishra, P., Mohakud, S."Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer". International Journal of Epigenetics 5.1 (2025): 5.
Chicago
Srujan, S., Sahu, S., Mishra, T. S., Muduly, D. K., Mishra, P., Mohakud, S."Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer". International Journal of Epigenetics 5, no. 1 (2025): 5. https://doi.org/10.3892/ije.2025.28
Copy and paste a formatted citation
x
Spandidos Publications style
Srujan S, Sahu S, Mishra TS, Muduly DK, Mishra P and Mohakud S: Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer. Int J Epigen 5: 5, 2025.
APA
Srujan, S., Sahu, S., Mishra, T.S., Muduly, D.K., Mishra, P., & Mohakud, S. (2025). Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer. International Journal of Epigenetics, 5, 5. https://doi.org/10.3892/ije.2025.28
MLA
Srujan, S., Sahu, S., Mishra, T. S., Muduly, D. K., Mishra, P., Mohakud, S."Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer". International Journal of Epigenetics 5.1 (2025): 5.
Chicago
Srujan, S., Sahu, S., Mishra, T. S., Muduly, D. K., Mishra, P., Mohakud, S."Diagnostic efficacy of circulating microRNAs‑27a, ‑122 and ‑155 in detecting early‑stage breast cancer". International Journal of Epigenetics 5, no. 1 (2025): 5. https://doi.org/10.3892/ije.2025.28
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