Characterization of the nuclear localization signal in the DNA helicase involved in Werner's syndrome.

  • Authors:
    • T Matsumoto
    • O Imamura
    • M Goto
    • Y Furuichi
  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/ijmm.1.1.71
  • Pages: 71-77
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Abstract

The nuclear localization signal (NLS) of the DNA helicase involved in Werner's syndrome (WS) was studied. Previously, we noted that the C-terminal region of WS helicase contains the NLS. In this study, we generated in HeLa cells various chimeric proteins consisting of the N-terminal tagged with an enhanced green fluorescent protein and the C-terminal fragments of the WS helicase that were truncated either from N- or C-termini, and we examined the ability of fragments to transfer the fusion proteins to the nucleoplasm by fluorescence microscopy. A small C-proximal region containing 34 amino acid residues (residues 1369-1402) was found to contain full nuclear migration activities. Subsequent amino acid substitution experiments showed that a sequence of three positively charged amino acids (Lys1371-Arg1372-Arg1373) in this region are particularly important. Similar sequence has previously been defined as the nuclear localization signal of SV-40 large T antigen that also acts as a viral DNA helicase. Conservation of this motif was found in the C-terminal regions of the other RecQ type DNA helicases, including murine WS helicase, yeast sgs1 and rqh+1 and human Bloom syndrome DNA helicases.

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Jan 1998
Volume 1 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Matsumoto T, Imamura O, Goto M and Furuichi Y: Characterization of the nuclear localization signal in the DNA helicase involved in Werner's syndrome.. Int J Mol Med 1: 71-77, 1998
APA
Matsumoto, T., Imamura, O., Goto, M., & Furuichi, Y. (1998). Characterization of the nuclear localization signal in the DNA helicase involved in Werner's syndrome.. International Journal of Molecular Medicine, 1, 71-77. https://doi.org/10.3892/ijmm.1.1.71
MLA
Matsumoto, T., Imamura, O., Goto, M., Furuichi, Y."Characterization of the nuclear localization signal in the DNA helicase involved in Werner's syndrome.". International Journal of Molecular Medicine 1.1 (1998): 71-77.
Chicago
Matsumoto, T., Imamura, O., Goto, M., Furuichi, Y."Characterization of the nuclear localization signal in the DNA helicase involved in Werner's syndrome.". International Journal of Molecular Medicine 1, no. 1 (1998): 71-77. https://doi.org/10.3892/ijmm.1.1.71