Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.

  • Authors:
    • A Ationu
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  • Published online on: June 1, 1998     https://doi.org/10.3892/ijmm.1.6.1007
  • Pages: 1007-1017
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Abstract

Accelerated transplant coronary artery disease (TxCAD) results in increased expression of antiendothelial antibodies whose target antigens remain largely unidentified. One of these endothelial antigens has been identified as vimentin, a cytoskeletal protein present in cells of the blood vessel walls. In the present study, SDS-PAGE and Western blot analysis of human endothelial cell (EAHy 926) lysates probed with sera from a TxCAD patient were used to confirm immunoreactivity of antiendothelial antibodies towards several endothelial proteins. To further elucidate the identity of these putative antigens, a human endothelial cell (EAHy 926) cDNA expression library was immunoscreened with serum obtained from a TxCAD patient. Two positive cDNA clones were identified by partial nucleotide sequence analysis and GenBank/EMBL database searches for homology as the 85 kDa human CD36 antigen (a cell surface glycoprotein expressed in various cells including epithelial and endothelial cells) and a 50 kDa keratin-like protein (a member of the intermediate filament protein expressed in epithelial cells). These results are the first to demonstrate that human CD36 antigen and a keratin-like protein may be additional target proteins for the anti-endothelial antibodies associated with TxCAD.

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Jun 1998
Volume 1 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ationu A: Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.. Int J Mol Med 1: 1007-1017, 1998
APA
Ationu, A. (1998). Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.. International Journal of Molecular Medicine, 1, 1007-1017. https://doi.org/10.3892/ijmm.1.6.1007
MLA
Ationu, A."Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.". International Journal of Molecular Medicine 1.6 (1998): 1007-1017.
Chicago
Ationu, A."Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.". International Journal of Molecular Medicine 1, no. 6 (1998): 1007-1017. https://doi.org/10.3892/ijmm.1.6.1007