Adrenomedullin and its receptors are expressed in the zona glomerulosa of immature and adult rat adrenals: Evidences of upregulation of ADM system in immature glands

  • Authors:
    • Piera Rebuffat
    • Myriam L. Forneris
    • Carlo Macchi
    • Ludwik K. Malendowicz
    • Marcin Trejter
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: July 1, 2002     https://doi.org/10.3892/ijmm.10.1.85
  • Pages: 85-88
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Abstract

Evidence has been provided that adrenomedullin (ADM) stimulates the proliferative activity of adult rat adrenal zona glomerulosa (ZG). However, the selective ADM receptor antagonist ADM22-52, although being able to block ADM effect, was per se ineffective. In contrast, in the companion paper, we showed that ADM22-52 depresses the proliferation rate of ZG in 20-day-old rats, suggesting the involvement of endogenous ADM system in adrenal maturation. Hence, we investigated by semiquantitative reverse transcription-polymerase chain reaction and radioimmune assay the expression of ADM system in adult and immature rat ZG. ProADM mRNA and ADM-immunoreactivity were both more elevated in immature- than adult-rat ZG. Plasma ADM concentration did not show significant age-related differences. ADM acts via two subtypes of ADM22-52-sensitive receptors: the L1 receptor (L1-R) and the calcitonin-receptor-like-receptor (CRLR), the latter behaves as selective ADM receptor only in the presence of the receptor-activity-modifying proteins (RAMPs)2 and 3. L1-R expression was enhanced in immature rat ZG, while CRLR and RAMP2,3 expression did not display significant differences. It is concluded that the endogenous ADM system located in the ZG is upregulated in immature rats, and plays an important autocrine-paracrine role in the maintenance of the elevated growth rate during adrenal maturation.

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July 2002
Volume 10 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Rebuffat P, Forneris ML, Macchi C, Malendowicz LK, Trejter M and Nussdorfer GG: Adrenomedullin and its receptors are expressed in the zona glomerulosa of immature and adult rat adrenals: Evidences of upregulation of ADM system in immature glands. Int J Mol Med 10: 85-88, 2002.
APA
Rebuffat, P., Forneris, M.L., Macchi, C., Malendowicz, L.K., Trejter, M., & Nussdorfer, G.G. (2002). Adrenomedullin and its receptors are expressed in the zona glomerulosa of immature and adult rat adrenals: Evidences of upregulation of ADM system in immature glands. International Journal of Molecular Medicine, 10, 85-88. https://doi.org/10.3892/ijmm.10.1.85
MLA
Rebuffat, P., Forneris, M. L., Macchi, C., Malendowicz, L. K., Trejter, M., Nussdorfer, G. G."Adrenomedullin and its receptors are expressed in the zona glomerulosa of immature and adult rat adrenals: Evidences of upregulation of ADM system in immature glands". International Journal of Molecular Medicine 10.1 (2002): 85-88.
Chicago
Rebuffat, P., Forneris, M. L., Macchi, C., Malendowicz, L. K., Trejter, M., Nussdorfer, G. G."Adrenomedullin and its receptors are expressed in the zona glomerulosa of immature and adult rat adrenals: Evidences of upregulation of ADM system in immature glands". International Journal of Molecular Medicine 10, no. 1 (2002): 85-88. https://doi.org/10.3892/ijmm.10.1.85