TNFα induces acetylation of p53 but attenuates its transcriptional activation in rheumatoid synoviocytes

  • Authors:
    • Minako Nakazawa
    • Satoko Aratani
    • Mitsutoki Hatta
    • Natsumi Araya
    • Hiroaki Daitoku
    • Ko-Ichi Kawahara
    • Shin-Ichi Watanabe
    • Hiroshi Nakamura
    • Shin-Ichi Yoshino
    • Ryouji Fujii
    • Hidetoshi Fujita
    • Akiyoshi Fukamizu
    • Kusuki Nishioka
    • Toshihiro Nakajima
  • View Affiliations

  • Published online on: September 1, 2002     https://doi.org/10.3892/ijmm.10.3.269
  • Pages: 269-275
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Abstract

Synovial hyperplasia is an important feature of rheumatoid arthritis (RA) and we have reported that several transcription factors were highly activated in rheumatoid synoviocytes. The purpose of this study was to examine nuclear acetylation in synoviocytes as an activation marker and determine its role in cell activation. Autonomous acetylation of approximately 53 and 62 kDa nuclear proteins was detected in rheumatoid synoviocytes by anti-acetylated lysine specific antibody. Furthermore, tumor necrosis factor α (TNFα), a potent mitogen for synoviocytes, dose-dependently increased their state of acetylation. Immunoprecipitation analysis revealed that 53 kDa acetylated protein (ap53) was identical with p53, a tumor suppressor gene product. Since enhanced p53 binding to the promoter by TNFα treatment was detected by gel shift assay, we analyzed p53 promoter activity by reporter assay system. Contrary to enhanced binding activity, the transcriptional activity was attenuated in a TNFα concentration-dependent manner. Since p53 activation requires recruitment of CREB binding protein (CBP) as a coactivator, we also examined the effect of CBP on TNFα-induced attenuation of p53 promoter activation. Overexpression of CBP induced p53 transcriptional activity and recovery of TNFα-induced inhibition. Our results clearly indicate that autonomous nuclear acetylation is characteristically enhanced in rheumatoid synoviocytes and that p53 is one of acetylated protein. Our results also demonstrate that TNFα-induced acetylation of p53 attenuated its transcriptional activation via CBP depletion, and that overexpression of CBP enhanced TNFα-induced cell death in rheumatoid synoviocytes, suggesting that regulation of transcriptional coactivator become a novel strategy for RA therapy.

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September 2002
Volume 10 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nakazawa M, Aratani S, Hatta M, Araya N, Daitoku H, Kawahara K, Watanabe S, Nakamura H, Yoshino S, Fujii R, Fujii R, et al: TNFα induces acetylation of p53 but attenuates its transcriptional activation in rheumatoid synoviocytes. Int J Mol Med 10: 269-275, 2002
APA
Nakazawa, M., Aratani, S., Hatta, M., Araya, N., Daitoku, H., Kawahara, K. ... Nakajima, T. (2002). TNFα induces acetylation of p53 but attenuates its transcriptional activation in rheumatoid synoviocytes. International Journal of Molecular Medicine, 10, 269-275. https://doi.org/10.3892/ijmm.10.3.269
MLA
Nakazawa, M., Aratani, S., Hatta, M., Araya, N., Daitoku, H., Kawahara, K., Watanabe, S., Nakamura, H., Yoshino, S., Fujii, R., Fujita, H., Fukamizu, A., Nishioka, K., Nakajima, T."TNFα induces acetylation of p53 but attenuates its transcriptional activation in rheumatoid synoviocytes". International Journal of Molecular Medicine 10.3 (2002): 269-275.
Chicago
Nakazawa, M., Aratani, S., Hatta, M., Araya, N., Daitoku, H., Kawahara, K., Watanabe, S., Nakamura, H., Yoshino, S., Fujii, R., Fujita, H., Fukamizu, A., Nishioka, K., Nakajima, T."TNFα induces acetylation of p53 but attenuates its transcriptional activation in rheumatoid synoviocytes". International Journal of Molecular Medicine 10, no. 3 (2002): 269-275. https://doi.org/10.3892/ijmm.10.3.269