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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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November 2002 Volume 10 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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November 2002 Volume 10 Issue 5

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Article

Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells

  • Authors:
    • S. Shimada
    • K. Yamaguchi
    • M. Takahashi
    • M. Ogawa
  • View Affiliations / Copyright

    Affiliations: Department of Surgery II, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan. shimadas443@hotmail.com
  • Pages: 599-603
    |
    Published online on: November 1, 2002
       https://doi.org/10.3892/ijmm.10.5.599
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Abstract

Ninety percent of pancreatic cancers are classified as ductal adenocarcinoma and are not known to secrete pancreatic elastases of the acinar enzymes. In the present study, we investigated the expression and the changes in elastase genes expressed in pancreatic ductal carcinoma cells. The expression of elastase gene family molecules in pancreatic cancer cells was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) method using primer sets for conservative active domains among pancreatic elastases (PEs) and neutophil elastase (NE). The PCR products were subcloned, sequenced and analyzed. Three distinct products were isolated in pancreatic carcinoma cells using the RT-PCR analyses. The sequencing revealed that one was identical with the PE IIIA isoform, and the remaining two were alternatively splicing forms of the PE IIIA. Four of five pancreatic cell lines expressed these splicing variants in a cell-dependent manner. The present study is the first report to demonstrate the expression of PE IIIA and its splicing variants in pancreatic carcinoma cells might represent another infiltrative feature of the pancreatic ductal cell carcinoma.

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Copy and paste a formatted citation
Spandidos Publications style
Shimada S, Yamaguchi K, Takahashi M and Ogawa M: Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int J Mol Med 10: 599-603, 2002.
APA
Shimada, S., Yamaguchi, K., Takahashi, M., & Ogawa, M. (2002). Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. International Journal of Molecular Medicine, 10, 599-603. https://doi.org/10.3892/ijmm.10.5.599
MLA
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10.5 (2002): 599-603.
Chicago
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10, no. 5 (2002): 599-603. https://doi.org/10.3892/ijmm.10.5.599
Copy and paste a formatted citation
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Spandidos Publications style
Shimada S, Yamaguchi K, Takahashi M and Ogawa M: Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int J Mol Med 10: 599-603, 2002.
APA
Shimada, S., Yamaguchi, K., Takahashi, M., & Ogawa, M. (2002). Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. International Journal of Molecular Medicine, 10, 599-603. https://doi.org/10.3892/ijmm.10.5.599
MLA
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10.5 (2002): 599-603.
Chicago
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10, no. 5 (2002): 599-603. https://doi.org/10.3892/ijmm.10.5.599
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