Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells

  • Authors:
    • S. Shimada
    • K. Yamaguchi
    • M. Takahashi
    • M. Ogawa
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijmm.10.5.599
  • Pages: 599-603
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Abstract

Ninety percent of pancreatic cancers are classified as ductal adenocarcinoma and are not known to secrete pancreatic elastases of the acinar enzymes. In the present study, we investigated the expression and the changes in elastase genes expressed in pancreatic ductal carcinoma cells. The expression of elastase gene family molecules in pancreatic cancer cells was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) method using primer sets for conservative active domains among pancreatic elastases (PEs) and neutophil elastase (NE). The PCR products were subcloned, sequenced and analyzed. Three distinct products were isolated in pancreatic carcinoma cells using the RT-PCR analyses. The sequencing revealed that one was identical with the PE IIIA isoform, and the remaining two were alternatively splicing forms of the PE IIIA. Four of five pancreatic cell lines expressed these splicing variants in a cell-dependent manner. The present study is the first report to demonstrate the expression of PE IIIA and its splicing variants in pancreatic carcinoma cells might represent another infiltrative feature of the pancreatic ductal cell carcinoma.

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November 2002
Volume 10 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shimada S, Yamaguchi K, Takahashi M and Ogawa M: Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int J Mol Med 10: 599-603, 2002
APA
Shimada, S., Yamaguchi, K., Takahashi, M., & Ogawa, M. (2002). Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. International Journal of Molecular Medicine, 10, 599-603. https://doi.org/10.3892/ijmm.10.5.599
MLA
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10.5 (2002): 599-603.
Chicago
Shimada, S., Yamaguchi, K., Takahashi, M., Ogawa, M."Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells". International Journal of Molecular Medicine 10, no. 5 (2002): 599-603. https://doi.org/10.3892/ijmm.10.5.599