Lipid-mediated gene transfection of intercellular adhesion molecule-1 suppresses the peritoneal metastasis of gastric carcinoma

  • Authors:
    • Hiroaki Tanaka
    • Masakazu Yashiro
    • Takeshi Sunami
    • Masaichi Ohira
    • K. Hirakawa-Y.S. Chung
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijmm.10.5.613
  • Pages: 613-617
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Abstract

We have previously reported that a decreased expression level of ICAM-1 in cancer cells frequently led to the development of lymph node metastasis, and suggested that ICAM-1 gene transfection may inhibit lymph node metastasis. In the present study, we investigated whether ICAM-1 gene therapy for the peritoneal metastasis of gastric carcinoma is useful as a new immuno-gene therapy. ICAM-1 gene was transfected into a gastric cancer cell line, OCUM-2MD3 (2MD3), which has a high metastatic ability to the peritoneum. A transfectant cancer cell line, 2MD3/ICAM-1, had high ICAM-1 expression on the cell surface. The adhesion and cytotoxicity abilities of peripheral blood mononuclear cells were significantly increased against 2MD3/ICAM-1 cells in comparison with 2MD3 cells. Mice inoculated with 2MD3/ICAM-1 cells in the peritoneal cavity had a significantly better survival rate than those inoculated with 2MD3 cells (log-rank test, p<0.05). Histologic findings revealed that more mononuclear cells existed around the metastatic nodules in 2MD3/ICAM-1. Although gastric carcinoma frequently causes peritoneal metastasis, no useful therapy for the metastasis of gastric carcinoma has been developed. These findings revealed that ICAM-1 gene transfection to cancer cells could be a useful immuno-gene therapy for the peritoneal metastasis of gastric carcinoma.

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November 2002
Volume 10 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanaka H, Yashiro M, Sunami T, Ohira M and Hirakawa-Y.S. Chung K: Lipid-mediated gene transfection of intercellular adhesion molecule-1 suppresses the peritoneal metastasis of gastric carcinoma. Int J Mol Med 10: 613-617, 2002.
APA
Tanaka, H., Yashiro, M., Sunami, T., Ohira, M., & Hirakawa-Y.S. Chung, K. (2002). Lipid-mediated gene transfection of intercellular adhesion molecule-1 suppresses the peritoneal metastasis of gastric carcinoma. International Journal of Molecular Medicine, 10, 613-617. https://doi.org/10.3892/ijmm.10.5.613
MLA
Tanaka, H., Yashiro, M., Sunami, T., Ohira, M., Hirakawa-Y.S. Chung, K."Lipid-mediated gene transfection of intercellular adhesion molecule-1 suppresses the peritoneal metastasis of gastric carcinoma". International Journal of Molecular Medicine 10.5 (2002): 613-617.
Chicago
Tanaka, H., Yashiro, M., Sunami, T., Ohira, M., Hirakawa-Y.S. Chung, K."Lipid-mediated gene transfection of intercellular adhesion molecule-1 suppresses the peritoneal metastasis of gastric carcinoma". International Journal of Molecular Medicine 10, no. 5 (2002): 613-617. https://doi.org/10.3892/ijmm.10.5.613