Adrenomedullin is expressed in cord blood hematopoietic cells and stimulates their clonal growth

  • Authors:
    • Laura Del Pup
    • Anna S. Belloni
    • Gianni Carraro
    • Sergio De Angeli
    • Pier Paolo Parnigotto
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijmm.11.2.157
  • Pages: 157-160
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Abstract

Adrenomedullin (AM) is a hypotensive peptide, which originates from the proteolytic cleavage of pro(p)AM and acts via AM22-52-sensitive receptors. Reverse transcription polymerase chain reaction allowed the detection of the specific mRNAs of pAM in the mononuclear hematopoietic cells of the cord blood and immunocytochemistry demonstrated their abundant AM-immunoreactivity. AM (10−8 M) markedly enhanced clonal growth of cord blood hematopoietic cells cultured on semisolid media added with stem cell-growth promoting cytokines, and this effect was abolished by AM22-52 (10−6 M). Collectively, these findings indicate that AM is expressed in and stimulates the proliferation of cord blood hematopoietic stem cells. Cord blood has been proposed as a source of stem cells alternative to bone marrow for allogeneic transplantation, and our study suggests that AM may be used, in addition to the classic cytokines, to expand in vitro cord blood stem cells in advance of their clinical use.

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February 2003
Volume 11 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Del Pup L, Belloni AS, Carraro G, De Angeli S, Parnigotto PP and Nussdorfer GG: Adrenomedullin is expressed in cord blood hematopoietic cells and stimulates their clonal growth. Int J Mol Med 11: 157-160, 2003
APA
Del Pup, L., Belloni, A.S., Carraro, G., De Angeli, S., Parnigotto, P.P., & Nussdorfer, G.G. (2003). Adrenomedullin is expressed in cord blood hematopoietic cells and stimulates their clonal growth. International Journal of Molecular Medicine, 11, 157-160. https://doi.org/10.3892/ijmm.11.2.157
MLA
Del Pup, L., Belloni, A. S., Carraro, G., De Angeli, S., Parnigotto, P. P., Nussdorfer, G. G."Adrenomedullin is expressed in cord blood hematopoietic cells and stimulates their clonal growth". International Journal of Molecular Medicine 11.2 (2003): 157-160.
Chicago
Del Pup, L., Belloni, A. S., Carraro, G., De Angeli, S., Parnigotto, P. P., Nussdorfer, G. G."Adrenomedullin is expressed in cord blood hematopoietic cells and stimulates their clonal growth". International Journal of Molecular Medicine 11, no. 2 (2003): 157-160. https://doi.org/10.3892/ijmm.11.2.157