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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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March 2003 Volume 11 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line

  • Authors:
    • Masaru Harada
    • Hiroto Kumemura
    • Shotaro Sakisaka
    • Shoichiro Shishido
    • Eitaro Taniguchi
    • Takumi Kawaguchi
    • Shinichiro Hanada
    • Hironori Koga
    • Ryukichi Kumashiro
    • Takato Ueno
    • Tatsuo Suganuma
    • Koh Furuta
    • Masayoshi Namba
    • Toshihiro Sugiyama
    • Michio Sata
  • View Affiliations / Copyright

    Affiliations: Second Department of Medicine, Kurume University School of Medicine, Kurume 830-0011, Japan. harada@med.kurume-u.ac.jp
  • Pages: 293-298
    |
    Published online on: March 1, 2003
       https://doi.org/10.3892/ijmm.11.3.293
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Abstract

Wilson disease is a genetic disorder characterized by the accumulation of copper in the body due to a defect of biliary copper excretion. The gene responsible for Wilson disease has been cloned, however, the precise localization of this gene product ATP7B, a copper-transporting ATPase, is still controversial. We examined the localization of ATP7B by expressing a chimeric protein, ATP7B-tagged with green fluorescent protein (GFP) (GFP-ATP7B), in HEK293, Hep3B and a highly polarized human hepatocyte line (OUMS29). Intracellular organelles were visualized by immunofluorescence microscopy. The effects of bathocuproine disulfonate, a copper chelator, and copper sulfate were examined. GFP-ATP7B colocalized with a late endosome marker, but not with endoplasmic reticulum, Golgi, or lysosome markers in a copper-depleting condition. Treatment with copper sulfate did not affect the localization of ATP7B. ATP7B is localized in the late endosomes in both copper-depleting and copper-loaded conditions. ATP7B seems to translocate copper from the cytosol into the late endosomes, and copper may be excreted to bile via lysosomes. We believe that the disturbed incorporation of copper into the late endosomes caused by mutated ATP7B is the main defect in Wilson disease.

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Copy and paste a formatted citation
Spandidos Publications style
Harada M, Kumemura H, Sakisaka S, Shishido S, Taniguchi E, Kawaguchi T, Hanada S, Koga H, Kumashiro R, Ueno T, Ueno T, et al: Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. Int J Mol Med 11: 293-298, 2003.
APA
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T. ... Sata, M. (2003). Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. International Journal of Molecular Medicine, 11, 293-298. https://doi.org/10.3892/ijmm.11.3.293
MLA
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T., Hanada, S., Koga, H., Kumashiro, R., Ueno, T., Suganuma, T., Furuta, K., Namba, M., Sugiyama, T., Sata, M."Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line". International Journal of Molecular Medicine 11.3 (2003): 293-298.
Chicago
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T., Hanada, S., Koga, H., Kumashiro, R., Ueno, T., Suganuma, T., Furuta, K., Namba, M., Sugiyama, T., Sata, M."Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line". International Journal of Molecular Medicine 11, no. 3 (2003): 293-298. https://doi.org/10.3892/ijmm.11.3.293
Copy and paste a formatted citation
x
Spandidos Publications style
Harada M, Kumemura H, Sakisaka S, Shishido S, Taniguchi E, Kawaguchi T, Hanada S, Koga H, Kumashiro R, Ueno T, Ueno T, et al: Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. Int J Mol Med 11: 293-298, 2003.
APA
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T. ... Sata, M. (2003). Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. International Journal of Molecular Medicine, 11, 293-298. https://doi.org/10.3892/ijmm.11.3.293
MLA
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T., Hanada, S., Koga, H., Kumashiro, R., Ueno, T., Suganuma, T., Furuta, K., Namba, M., Sugiyama, T., Sata, M."Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line". International Journal of Molecular Medicine 11.3 (2003): 293-298.
Chicago
Harada, M., Kumemura, H., Sakisaka, S., Shishido, S., Taniguchi, E., Kawaguchi, T., Hanada, S., Koga, H., Kumashiro, R., Ueno, T., Suganuma, T., Furuta, K., Namba, M., Sugiyama, T., Sata, M."Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line". International Journal of Molecular Medicine 11, no. 3 (2003): 293-298. https://doi.org/10.3892/ijmm.11.3.293
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