Mucosal permeability regulates receptor binding of luminal epidermal growth factor in the adult rat intestine

Tsujikawa,Tomoyuki; Itoh,Akihiko; Yasuoka,Takashi; Fukunaga,Tetsuya; Satoh,Jin; Uda,Katsuhiro; Ihara,Takashi; Sasaki,Masaya; Fujiyama,Yoshihide

doi:10.3892/ijmm.11.3.349

March 2003 Volume 11 Issue 3

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March 2003 Volume 11 Issue 3

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Article

Mucosal permeability regulates receptor binding of luminal epidermal growth factor in the adult rat intestine

Authors:
- Tomoyuki Tsujikawa
- Akihiko Itoh
- Takashi Yasuoka
- Tetsuya Fukunaga
- Jin Satoh
- Katsuhiro Uda
- Takashi Ihara
- Masaya Sasaki
- Yoshihide Fujiyama
View Affiliations / Copyright

Affiliations: Division of Gastroenterology, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan. tujikawa@belle.shiga-med.ac.jp
Pages: 349-352
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Published online on: March 1, 2003

https://doi.org/10.3892/ijmm.11.3.349
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Abstract

Epidermal growth factor (EGF) stimulates repair in the damaged intestine, but its role in the normal intestine is not clear. Because EGF receptors are found on the basolateral surface but not the luminal surface, we hypothesized that mucosal permeability regulates EGF binding. Adult male rats were divided into 3 groups, one that was fed normal chow (the control), one that was starved for 4 days, and one that was given methotrexate (MTX) intragastrically (10 mg/kg/day for 3 days). The rats were sacrificed and everted sacks of the jejunum were made and incubated in EGF solution. Western blot analysis of mucosal homogenates showed that the amount of phosphotyrosyl EGF receptor in the starved and MTX-treated groups was, respectively, about 1.5 times and 2 times that in the control group. The mucosal permeability in the starved and MTX treated groups also increased and varied directly with the amount of phosphotyrosyl EGF receptor. These results suggest that in the adult rat intestine, luminal EGF may play a role only under tissue damage, where enhanced permeability permits the EGF to pass through the mucosa and bind to its receptor on the basolateral membrane.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Mucosal permeability regulates receptor binding of luminal epidermal growth factor in the adult rat intestine

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