Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma

  • Authors:
    • Er-Bin Yang
    • Liu-Liang Qin
    • Yin-Nong Zhao
    • Kai Zhang
    • Pierce Chow
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijmm.11.6.773
  • Pages: 773-778
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Abstract

It was reported that 60-70% of hepatitis B virus (HBV)-negative hepatocellular carcinoma (HCC) had loss of heterozygosity (LOH) at the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) locus and this gene was mutated in 55% of these patients with LOH. In this study, genomic DNA from 29 pairs of HBV-positive HCC and corresponding non-tumor tissues was used to analyze LOH at the M6P/IGF2R locus and single deoxyguanosine deletion in this gene by PCR. Total RNA from 19 of the 29 patients was utilized to determine a 192 bp insert in the M6P/IGF2R mRNA and expression of this gene by RT-PCR. Twenty-eight of 29 (97%) HBV-positive HCC were found to be informative at the M6P/IGF2R locus but LOH at this region was only detected in 4/28 (14%) informative patients. Neither single deoxyguanosine deletion in this gene nor 192 bp insert in its mRNA occurred in these patients. Compared with corresponding non-tumor tissues, expression of the M6P/IGF2R mRNA was decreased in 13/19 (68%) HBV-positive HCC tissues, suggesting that M6P/IGF2R may be involved in HBV-associated hepatocarcinogenesis by the regulation of its expression level. In the development of HBV-associated HCC, M6P/IGF2R mutation may not be a major agent.

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June 2003
Volume 11 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang E, Qin L, Zhao Y, Zhang K and Chow P: Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma. Int J Mol Med 11: 773-778, 2003
APA
Yang, E., Qin, L., Zhao, Y., Zhang, K., & Chow, P. (2003). Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma. International Journal of Molecular Medicine, 11, 773-778. https://doi.org/10.3892/ijmm.11.6.773
MLA
Yang, E., Qin, L., Zhao, Y., Zhang, K., Chow, P."Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma". International Journal of Molecular Medicine 11.6 (2003): 773-778.
Chicago
Yang, E., Qin, L., Zhao, Y., Zhang, K., Chow, P."Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma". International Journal of Molecular Medicine 11, no. 6 (2003): 773-778. https://doi.org/10.3892/ijmm.11.6.773