Inhibitory effect of pyridoxal 5'-phosphate on endothelial cell proliferation, replicative DNA polymerase and DNA topoisomerase

  • Authors:
    • Kiminori Matsubara
    • Hiroaki Matsumoto
    • Yoshiyuki Mizushina
    • Jung Sup Lee
    • Norihisa Kato
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijmm.12.1.51
  • Pages: 51-55
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Abstract

Recently, the anti-angiogenic effect of pyridoxal 5'-phosphate (PLP) and pyridoxal (PL) was demonstrated in an ex vivo serum-free matrix culture model using rat aortic ring. To clarify how vitamin B6 inhibits angiogenesis, this study was performed to examine the effect on human umbilical vein endothelial cell (HUVEC) proliferation and HUVEC tube formation. Consistent with the result on an ex vivo angiogenesis assay, PLP and PL at 250 μM markedly suppressed the proliferation of HUVEC, while pyridoxine (PN) and pyridoxamine (PM) were inactive at this concentration. Suppression in HUVEC proliferation by PLP and PL was evident in a dose-dependent manner within the range of 50-250 μM. However, the HUVEC tube formation was unaffected by PLP and PL. The activities of replicative DNA polymerase and DNA topoisomerases I and II were also inhibited by PLP. These results suggest that vitamin B6 suppresses endothelial cell proliferation and angiogenesis at least in part by inhibiting DNA polymerase and DNA topoisomerases.

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July 2003
Volume 12 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Matsubara K, Matsumoto H, Mizushina Y, Lee JS and Kato N: Inhibitory effect of pyridoxal 5'-phosphate on endothelial cell proliferation, replicative DNA polymerase and DNA topoisomerase. Int J Mol Med 12: 51-55, 2003.
APA
Matsubara, K., Matsumoto, H., Mizushina, Y., Lee, J.S., & Kato, N. (2003). Inhibitory effect of pyridoxal 5'-phosphate on endothelial cell proliferation, replicative DNA polymerase and DNA topoisomerase. International Journal of Molecular Medicine, 12, 51-55. https://doi.org/10.3892/ijmm.12.1.51
MLA
Matsubara, K., Matsumoto, H., Mizushina, Y., Lee, J. S., Kato, N."Inhibitory effect of pyridoxal 5'-phosphate on endothelial cell proliferation, replicative DNA polymerase and DNA topoisomerase". International Journal of Molecular Medicine 12.1 (2003): 51-55.
Chicago
Matsubara, K., Matsumoto, H., Mizushina, Y., Lee, J. S., Kato, N."Inhibitory effect of pyridoxal 5'-phosphate on endothelial cell proliferation, replicative DNA polymerase and DNA topoisomerase". International Journal of Molecular Medicine 12, no. 1 (2003): 51-55. https://doi.org/10.3892/ijmm.12.1.51