Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells

  • Authors:
    • Hiroshige Hibasami
    • Yayoi Yamada
    • Hiroyuki Moteki
    • Hirotaka Katsuzaki
    • Kunio Imai
    • Kazumi Yoshioka
    • Takashi Komiya
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijmm.12.2.147
  • Pages: 147-151
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Abstract

Sesquiterpenes (costunolide and zaluzanin D) were purified from laurel (Laurus nobilis L.) and identified by Mass, and 1H- and 13C-NMR. These sesquiterpenes displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological changes of the nucleus, including chromatin condensation were induced in the HL-60 cells treated with the sesquiterpenes. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 11.4, 47.0, and 92.5% after a 3-day-treatment with 5, 10 and 15 μM costunolide, respectively. The same analysis showed that the hypodiploid nuclei of HL 60 cells were increased to 12.4, 28.9 and 76.7% after a 3-day-treatment with 10, 15 and 20 μM zaluzanin D, respectively. These findings suggest that growth inhibition by costunolide and zaluzanin D of HL-60 cells results from the induction of chromatin condensation in the HL-60 cells. On the other hand, fragmentations by these compounds of DNA to oligonucleosomal-sized fragments were not observed in the HL-60 cells.

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August 2003
Volume 12 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hibasami H, Yamada Y, Moteki H, Katsuzaki H, Imai K, Yoshioka K and Komiya T: Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells. Int J Mol Med 12: 147-151, 2003
APA
Hibasami, H., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., Yoshioka, K., & Komiya, T. (2003). Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells. International Journal of Molecular Medicine, 12, 147-151. https://doi.org/10.3892/ijmm.12.2.147
MLA
Hibasami, H., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., Yoshioka, K., Komiya, T."Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells". International Journal of Molecular Medicine 12.2 (2003): 147-151.
Chicago
Hibasami, H., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., Yoshioka, K., Komiya, T."Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells". International Journal of Molecular Medicine 12, no. 2 (2003): 147-151. https://doi.org/10.3892/ijmm.12.2.147