Aromatic residues are required for RNA helicase A mediated transactivation

  • Authors:
    • Satoko Aratani
    • Ryouji Fujii
    • Hidetoshi Fujita
    • Akiyoshi Fukamizu
    • Toshihiro Nakajima
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijmm.12.2.175
  • Pages: 175-180
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Abstract

RNA helicase A (RHA) is a member of ATPase/helicase and regulates the transcription through recruitment of Pol II and/or by ATP dependent mechanisms. In CREB-dependent transcription, RHA recruits RNA polymerase (Pol) II to the CREB binding protein (CBP) via the minimal transactivation domain (MTAD). This region is well conserved among RHA homologues, whereas it is unique to RHA. The three conserved tryptophan residues in MTAD are critical for transactivation. To understand the importance of tryptophan residues on transactivation, we generated mutants in which tryptophan residues were replaced by other aromatic, bulky hydrophobic or small hydrophobic amino acids. Substitutions of tryptophan with either bulky hydrophobic or small hydrophobic amino acid decreased transcriptional activity, whereas aromatic residue had no effect. Moreover, these mutants with tryptophan to phenylalanine, activated CREB-dependent transcription. These results indicate that aromatic characteristics of tryptophan residues in MTAD are important for CREB-dependent transcription via RHA.

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August 2003
Volume 12 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Aratani S, Fujii R, Fujita H, Fukamizu A and Nakajima T: Aromatic residues are required for RNA helicase A mediated transactivation. Int J Mol Med 12: 175-180, 2003.
APA
Aratani, S., Fujii, R., Fujita, H., Fukamizu, A., & Nakajima, T. (2003). Aromatic residues are required for RNA helicase A mediated transactivation. International Journal of Molecular Medicine, 12, 175-180. https://doi.org/10.3892/ijmm.12.2.175
MLA
Aratani, S., Fujii, R., Fujita, H., Fukamizu, A., Nakajima, T."Aromatic residues are required for RNA helicase A mediated transactivation". International Journal of Molecular Medicine 12.2 (2003): 175-180.
Chicago
Aratani, S., Fujii, R., Fujita, H., Fukamizu, A., Nakajima, T."Aromatic residues are required for RNA helicase A mediated transactivation". International Journal of Molecular Medicine 12, no. 2 (2003): 175-180. https://doi.org/10.3892/ijmm.12.2.175