Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes

  • Authors:
    • Jeong-Hwa Baek
    • Gene Lee
    • Si Nae Kim
    • Jin-Man Kim
    • Moonkyu Kim
    • Sung-Chang Chung
    • Byung-Moo Min
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/ijmm.12.3.319
  • Pages: 319-325
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Serial subcultures of normal human oral keratinocytes (NHOKs) and normal human epidermal keratinocytes (NHEKs) to the postmitotic stage result in terminal differentiation and replicative senescence. In order to investigate the common mode of differentiation and/or senescence between mucosal and epidermal keratinocytes, gene expression profiling on both NHOKs and NHEKs was performed by a cDNA microarray analysis. Primary NHOKs and NHEKs were serially subcultured, and the expression level of 3,063 genes was compared between the exponentially growing and senescent cultures. The senescent NHOKs and NHEKs highly expressed 55 and 37 genes, respectively. Among these genes, 16 genes were common in both NHOKs and NHEKs while the other genes were upregulated either in the NHOKs or in the NHEKs. Furthermore, the expression levels of the common genes did not change in the human diploid fibroblasts during the subcultures. These results suggest that subculture-induced differentiation and/or replicative senescence in NHOKs and NHEKs has similar characteristics, but that the pathways leading to these processes are distinct and keratinocyte specific.

Related Articles

Journal Cover

September 2003
Volume 12 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Baek, J., Lee, G., Kim, S.N., Kim, J., Kim, M., Chung, S., & Min, B. (2003). Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes. International Journal of Molecular Medicine, 12, 319-325. https://doi.org/10.3892/ijmm.12.3.319
MLA
Baek, J., Lee, G., Kim, S. N., Kim, J., Kim, M., Chung, S., Min, B."Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes". International Journal of Molecular Medicine 12.3 (2003): 319-325.
Chicago
Baek, J., Lee, G., Kim, S. N., Kim, J., Kim, M., Chung, S., Min, B."Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes". International Journal of Molecular Medicine 12, no. 3 (2003): 319-325. https://doi.org/10.3892/ijmm.12.3.319