A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells

  • Authors:
    • Aslamuzzaman Kazi
    • Daniel A. Urbizu
    • Deborah J. Kuhn
    • Abelardo L. Acebo
    • Edward R. Jackson
    • Gail P. Greenfelder
    • Nagi B. Kumar
    • Q. Ping Dou
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijmm.12.6.879
  • Pages: 879-887
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Abstract

Animal studies have demonstrated that a dietary polyphenol known as tannic acid (TA) exhibits anticarcinogenic activity in chemically induced cancers. Most recently, we have reported that TA and ester-bond containing green tea polyphenols are potent proteasome inhibitors in vitro and in vivo. We hypothesize that CellQuest™, a patented formula which contains high level of TA obtained from a musaceas (plantain) plant extract, will inhibit the tumor cell proteasome activity. Here, we report that a partially purified CellQuest fraction, S3, potently inhibits the proteasomal chymotrypsin-like activity of Jurkat T cell extracts in a concentration-dependent manner. Inhibition of the proteasome by S3 in leukemia Jurkat T, simian virus 40-transformed and prostate cancer LNCaP cells results in accumulation of ubiquitinated proteins and the natural proteasome substrate p27Kip1, followed by induction of apoptosis. In contrast, non-transformed, immortalized human natural killer cells and normal human fibroblasts are resistant to S3-mediated proteasome inhibition and apoptosis induction. Our present study suggests that CellQuest targets and inhibits the proteasome selectively in tumor cells, which may contribute to the claimed anticancer activity.

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December 2003
Volume 12 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kazi A, Urbizu DA, Kuhn DJ, Acebo AL, Jackson ER, Greenfelder GP, Kumar NB and Dou QP: A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells. Int J Mol Med 12: 879-887, 2003
APA
Kazi, A., Urbizu, D.A., Kuhn, D.J., Acebo, A.L., Jackson, E.R., Greenfelder, G.P. ... Dou, Q.P. (2003). A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells. International Journal of Molecular Medicine, 12, 879-887. https://doi.org/10.3892/ijmm.12.6.879
MLA
Kazi, A., Urbizu, D. A., Kuhn, D. J., Acebo, A. L., Jackson, E. R., Greenfelder, G. P., Kumar, N. B., Dou, Q. P."A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells". International Journal of Molecular Medicine 12.6 (2003): 879-887.
Chicago
Kazi, A., Urbizu, D. A., Kuhn, D. J., Acebo, A. L., Jackson, E. R., Greenfelder, G. P., Kumar, N. B., Dou, Q. P."A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells". International Journal of Molecular Medicine 12, no. 6 (2003): 879-887. https://doi.org/10.3892/ijmm.12.6.879