Identification of a novel DNase I hypersensitive site within the far upstream region of the human HLA-DRA gene

  • Authors:
    • Marina Tomassetti
    • Giordana Feriotto
    • Patrizio Giacomini
    • Ezio Giorda
    • Nicoletta Bianchi
    • Monica Borgatti
    • Alessia Finotti
    • Carlo Mischiati
    • Roberto Gambari
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijmm.12.6.929
  • Pages: 929-934
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Abstract

The class II products of the major histocompatibility complex have a distribution restricted to certain tissues and cells. For instance, they are constitutively expressed by B lymphocytes, but not by resting T lymphocytes. In this study, we report the identification of a novel DNase I hypersensitive site within a putative regulatory region of the human HLA-DRA gene, the so-called far upstream region. This hypersensitive site was present in the genome of the DRα-positive human B-lymphoid Raji cell line, and absent in the DRα-negative T-lymphoid Jurkat cell line. In addition, this hypersensitive site was also present in transgenic B lymphocytes isolated from the murine transgenic line TG 53, carrying a single integrated copy of the human HLA-DRA gene per haploid genome. The correlation between DRA expression and the presence of this far upstream hypersensitive site suggests novel long distance chromatin remodeling mechanisms possibly shared by human and murine class II genes.

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December 2003
Volume 12 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tomassetti M, Feriotto G, Giacomini P, Giorda E, Bianchi N, Borgatti M, Finotti A, Mischiati C and Gambari R: Identification of a novel DNase I hypersensitive site within the far upstream region of the human HLA-DRA gene. Int J Mol Med 12: 929-934, 2003.
APA
Tomassetti, M., Feriotto, G., Giacomini, P., Giorda, E., Bianchi, N., Borgatti, M. ... Gambari, R. (2003). Identification of a novel DNase I hypersensitive site within the far upstream region of the human HLA-DRA gene. International Journal of Molecular Medicine, 12, 929-934. https://doi.org/10.3892/ijmm.12.6.929
MLA
Tomassetti, M., Feriotto, G., Giacomini, P., Giorda, E., Bianchi, N., Borgatti, M., Finotti, A., Mischiati, C., Gambari, R."Identification of a novel DNase I hypersensitive site within the far upstream region of the human HLA-DRA gene". International Journal of Molecular Medicine 12.6 (2003): 929-934.
Chicago
Tomassetti, M., Feriotto, G., Giacomini, P., Giorda, E., Bianchi, N., Borgatti, M., Finotti, A., Mischiati, C., Gambari, R."Identification of a novel DNase I hypersensitive site within the far upstream region of the human HLA-DRA gene". International Journal of Molecular Medicine 12, no. 6 (2003): 929-934. https://doi.org/10.3892/ijmm.12.6.929