Natural cell-mediated cytotoxicity of peripheral blood lymphocytes against target cells transfected with epidermodysplasia verruciformis-specific human papillomavirus type 8 L1 DNA sequences

Oldak,Monika; Majewski,Slawomir; Grzela,Tomasz; Stark,Sabine; Malejczyk,Magdalena; Osiecka-Iwan,Anna; Fuchs,Pawel G.; Jablonska,Stefania; Pfister,Herbert J.; Malejczyk,Jacek

doi:10.3892/ijmm.13.1.187

January 2004 Volume 13 Issue 1

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January 2004 Volume 13 Issue 1

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Article

Natural cell-mediated cytotoxicity of peripheral blood lymphocytes against target cells transfected with epidermodysplasia verruciformis-specific human papillomavirus type 8 L1 DNA sequences

Authors:
- Monika Oldak
- Slawomir Majewski
- Tomasz Grzela
- Sabine Stark
- Magdalena Malejczyk
- Anna Osiecka-Iwan
- Pawel G. Fuchs
- Stefania Jablonska
- Herbert J. Pfister
- Jacek Malejczyk
View Affiliations / Copyright

Affiliations: Laboratory for Molecular Cell Biology, Department of Histology and Embryology, Center of Biostructure Research, Medical University of Warsaw, PL-02004 Warsaw, Poland. monika.oldak@ib.amwaw.edu.pl
Pages: 187-191
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Published online on: January 1, 2004

https://doi.org/10.3892/ijmm.13.1.187
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Abstract

The aim of the present study was to characterise natural cell-mediated cytotoxicity against COS-7 cells transfected with potentially oncogenic HPV-8 L1 DNA sequences cloned in sense and antisense orientation and to evaluate their lysis by peripheral blood lymphocytes (PBL) from patients with epidermodysplasia verruciformis (EV), a rare disease associated with life-long infection by specific HPV types. COS-7 cells were transfected with HPV-8 Hinc II restriction fragment (nucleotide positions 5434-7654) cloned in sense (COS-L1S) and antisense (COS-L1A) orientation into pCB6 expression vector. Cytotoxic activity of isolated PBL against COS cell lines as well as K562 erythroleukaemic cells was evaluated by 51Cr-release assay. We found that lymphocytes responsible for natural lysis of COS and K562 cells are CD3-negative CD56-positive natural killer (NK) cells. Analysis of NK cell cytotoxic activity against different COS cell lines has revealed that lymphocytes from healthy subjects killed COS-L1S cells significantly more efficiently than wild COS-7 and COS-L1A cells. Significantly more efficient lysis of COS-L1S cells was also observed in EV patients. Thus, expression of HPV L1 renders target cells more susceptible to NK-mediated cytotoxicity that may enable more effective elimination of transformed cells.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Natural cell-mediated cytotoxicity of peripheral blood lymphocytes against target cells transfected with epidermodysplasia verruciformis-specific human papillomavirus type 8 L1 DNA sequences

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Abstract

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