pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis

  • Authors:
    • Akira Hasegawa
    • Masahiro Kuroda
    • Yadi Wang
    • Shiro Akaki
    • Jun-Ichi Asaumi
    • Kohichi Shibuya
    • Shoji Kawasaki
    • Yoshihiro Okumura
    • Susumu Kanazawa
    • Yoshio Hiraki
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/ijmm.13.2.235
  • Pages: 235-242
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.

Related Articles

Journal Cover

February 2004
Volume 13 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hasegawa A, Kuroda M, Wang Y, Akaki S, Asaumi J, Shibuya K, Kawasaki S, Okumura Y, Kanazawa S, Hiraki Y, Hiraki Y, et al: pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis. Int J Mol Med 13: 235-242, 2004.
APA
Hasegawa, A., Kuroda, M., Wang, Y., Akaki, S., Asaumi, J., Shibuya, K. ... Hiraki, Y. (2004). pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis. International Journal of Molecular Medicine, 13, 235-242. https://doi.org/10.3892/ijmm.13.2.235
MLA
Hasegawa, A., Kuroda, M., Wang, Y., Akaki, S., Asaumi, J., Shibuya, K., Kawasaki, S., Okumura, Y., Kanazawa, S., Hiraki, Y."pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis". International Journal of Molecular Medicine 13.2 (2004): 235-242.
Chicago
Hasegawa, A., Kuroda, M., Wang, Y., Akaki, S., Asaumi, J., Shibuya, K., Kawasaki, S., Okumura, Y., Kanazawa, S., Hiraki, Y."pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis". International Journal of Molecular Medicine 13, no. 2 (2004): 235-242. https://doi.org/10.3892/ijmm.13.2.235