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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February 2004 Volume 13 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2004 Volume 13 Issue 2

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Article

Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture

  • Authors:
    • Stefan Marlovits
    • Matthias Hombauer
    • Dietmar Tamandl
    • Vilmos Vècsei
    • Werner Schlegel

  • View Affiliations / Copyright

    Affiliations: Department of Traumatology, University of Vienna, A-1090 Vienna, Austria. stefan.marlovits@akh-wien.ac.at
  • Pages: 281-287
    |
    Published online on: February 1, 2004
       https://doi.org/10.3892/ijmm.13.2.281
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Abstract

Human articular chondrocytes (HACs) were isolated from cartilage samples of normal joints and cultivated in monolayer for 56 days. Collagen type I, II, IX, aggrecan, and versican expression was quantified by real-time polymerase chain reaction (PCR). The expression of the genes was highly time-dependent and changed over the culture time. Collagen type I was not present at the beginning of the culture and increased 100-fold during the culture time. Collagen type II and IX expression was found during the entire culture period and decreased more than 100-fold. Aggrecan expression was downregulated 100-fold whereas versican expression increased 10 times. Indices of cell differentiation, defined as ratios of collagen type II to I (CII/I), collagen type IX to I (CIX/I), and aggrecan to versican (Agg/Ver), were significantly higher at the beginning of the culture and decreased over the culture period. The CII/I and CIX/I ratios formed three phases, with a plateau at the beginning, followed by a transition phase and a steady state at the end of the culture time, whereas the Agg/Ver ratio declined constantly. The accurate quantitative assessment of extracellular matrix gene expression in samples of chondrocytes taken from monolayer culture can be used to monitor chondrocyte metabolism as well as changes in the cell differentiation status.

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Copy and paste a formatted citation
Spandidos Publications style
Marlovits S, Hombauer M, Tamandl D, Vècsei V and Schlegel W: Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture. Int J Mol Med 13: 281-287, 2004.
APA
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., & Schlegel, W. (2004). Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture. International Journal of Molecular Medicine, 13, 281-287. https://doi.org/10.3892/ijmm.13.2.281
MLA
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., Schlegel, W."Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture". International Journal of Molecular Medicine 13.2 (2004): 281-287.
Chicago
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., Schlegel, W."Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture". International Journal of Molecular Medicine 13, no. 2 (2004): 281-287. https://doi.org/10.3892/ijmm.13.2.281
Copy and paste a formatted citation
x
Spandidos Publications style
Marlovits S, Hombauer M, Tamandl D, Vècsei V and Schlegel W: Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture. Int J Mol Med 13: 281-287, 2004.
APA
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., & Schlegel, W. (2004). Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture. International Journal of Molecular Medicine, 13, 281-287. https://doi.org/10.3892/ijmm.13.2.281
MLA
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., Schlegel, W."Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture". International Journal of Molecular Medicine 13.2 (2004): 281-287.
Chicago
Marlovits, S., Hombauer, M., Tamandl, D., Vècsei, V., Schlegel, W."Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture". International Journal of Molecular Medicine 13, no. 2 (2004): 281-287. https://doi.org/10.3892/ijmm.13.2.281
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