Different muscarinc receptors are involved in the proliferation of murine mammary adenocarcinoma cell lines

  • Authors:
    • Alejandro J. Español
    • María E. Sales
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/ijmm.13.2.311
  • Pages: 311-317
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Abstract

We described that two different murine mammary adenocarcinoma cell lines, LM3 and LM2 constitutively expressed muscarinic acetylcholine receptors (mAchR). We here demonstrate, by competitive binding experiments with the tritiated muscarinic antagonist quinuclidinyl benzilate that M2 subtype predominates in both tumor cell lines. Concordantly immunoblotting assays indicate that mAchR exhibit the following order of expression: M2 > M4 > M3 > M1 >> M5 in both tumor cell lines. Activation of mAchR with carbachol (CARB) increased proliferation in both tumor cell lines in a concentration dependent manner. In LM3 cells CARB promoted proliferation via M3 receptor activation via inositol 1,4,5-triphosphate and nitric oxide production. CARB-induced LM2 cells proliferation needed both M2 and M1 receptor activation, promoting prostaglandin E2 liberation and arginase catabolism respectively, both of them involved in tumor cell growth.

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February 2004
Volume 13 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Español AJ and Sales ME: Different muscarinc receptors are involved in the proliferation of murine mammary adenocarcinoma cell lines. Int J Mol Med 13: 311-317, 2004.
APA
Español, A.J., & Sales, M.E. (2004). Different muscarinc receptors are involved in the proliferation of murine mammary adenocarcinoma cell lines. International Journal of Molecular Medicine, 13, 311-317. https://doi.org/10.3892/ijmm.13.2.311
MLA
Español, A. J., Sales, M. E."Different muscarinc receptors are involved in the proliferation of murine mammary adenocarcinoma cell lines". International Journal of Molecular Medicine 13.2 (2004): 311-317.
Chicago
Español, A. J., Sales, M. E."Different muscarinc receptors are involved in the proliferation of murine mammary adenocarcinoma cell lines". International Journal of Molecular Medicine 13, no. 2 (2004): 311-317. https://doi.org/10.3892/ijmm.13.2.311