Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats
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- Published online on: May 1, 2004 https://doi.org/10.3892/ijmm.13.5.697
- Pages: 697-703
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Abstract
This study aims at assessing the conversion of exogenous D-__AMB__lsqb;1-13C__AMB__rsqb;fructose, D-__AMB__lsqb;2-13C__AMB__rsqb;fructose or D-__AMB__lsqb;6-13C__AMB__rsqb;-fructose (10 mM) to 13C-enriched and either hydrogenated or deuterated D-glucose, L-lactate and L-alanine released by rat liver cells prepared from Goto-Kakizaki rats and incubated for 120 min in the presence of unlabelled D-glucose (also 10 mM) and D2O. The results of this study are relevant to the relative contribution of fructokinase and hexokinase isoenzyme to the phosphorylation of D-fructose, the capacity of D-glucose to confer to glucokinase positive cooperativity towards D-fructose, the circulation of D-fructose 6-phosphate in the pentose phosphate pathway, the regulation of the cytosolic NADD/NADH ratio, the respective fate of D-fructose-derived D-glyceraldehyde and dihydroxyacetone phosphate, the deuteration of fructose-derived glycolytic intermediates at the phosphoglucoisomerase, phosphomannoisomerase, enolase, pyruvate kinase and glutamate-alanine transaminase levels, and the unequal generation of L-__AMB__lsqb;1-13C__AMB__rsqb;lactate by cells exposed to D-__AMB__lsqb;1-13C__AMB__rsqb;fructose or D-__AMB__lsqb;6-13C__AMB__rsqb;fructose versus D-__AMB__lsqb;2-13C__AMB__rsqb;-fructose.