An aqueous extract of Platycodi radix inhibits LPS-induced NF-κB nuclear translocation in human cultured airway epithelial cells

  • Authors:
    • Jun-Hyuk Lee
    • Yung-Hyun Choi
    • Ho-Sung Kang
    • Byung-Tae Choi
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijmm.13.6.843
  • Pages: 843-847
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Abstract

We investigated the effects of aqueous extract from Platycodi radix (AEPR), a traditional drug used to treat acute lung inflammatory disease, on lipopolysaccharide (LPS)-induced inflammation in A549 human cultured airway epithelial cells. Nuclear factor-κB (NF-κB) and its inhibitory regulator, inhibitory κB (I-κB), play crucial roles in LPS-induced inflammatory response. We show that LPS-induced nuclear translocation of NF-κBp65 is inhibited by AEPR. LPS-induced expression of I-κBα, which is expressed by LPS-induced activation of NF-κB, is inhibited by AEPR as well. Besides LPS-induced expression of a group of genes, such as tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), are repressed by AEPR. We also found that expression of heat shock protein 70 (Hsp70), which has an anti-inflammatory activity, is increased by AEPR plus LPS. These results suggest that AEPR may act as a therapeutic agent for inflammatory disease through regulating the activity of NF-κB and expression of inflammatory genes.

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June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee J, Choi Y, Kang H and Choi B: An aqueous extract of Platycodi radix inhibits LPS-induced NF-κB nuclear translocation in human cultured airway epithelial cells. Int J Mol Med 13: 843-847, 2004
APA
Lee, J., Choi, Y., Kang, H., & Choi, B. (2004). An aqueous extract of Platycodi radix inhibits LPS-induced NF-κB nuclear translocation in human cultured airway epithelial cells. International Journal of Molecular Medicine, 13, 843-847. https://doi.org/10.3892/ijmm.13.6.843
MLA
Lee, J., Choi, Y., Kang, H., Choi, B."An aqueous extract of Platycodi radix inhibits LPS-induced NF-κB nuclear translocation in human cultured airway epithelial cells". International Journal of Molecular Medicine 13.6 (2004): 843-847.
Chicago
Lee, J., Choi, Y., Kang, H., Choi, B."An aqueous extract of Platycodi radix inhibits LPS-induced NF-κB nuclear translocation in human cultured airway epithelial cells". International Journal of Molecular Medicine 13, no. 6 (2004): 843-847. https://doi.org/10.3892/ijmm.13.6.843