Dissimilar effects of D-mannoheptulose on the phosphorylation of α- versus β-D-glucose by either hexokinase or glucokinase

  • Authors:
    • Ying Zhang
    • Philippe Courtois
    • Abdullah Sener
    • Willy J. Malaisse
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.1.107
  • Pages: 107-112
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Abstract

D-mannoheptulose inhibits D-glucose phosphorylation by hexokinase isoenzymes. The present study aims at investigating whether the pattern of such an inhibition differs in the case of α- versus β-D-glucose. The phosphorylation of α- and β-D-[U-14C]glucose was measured over 60-min incubation at 4°C in the presence of bovine heart hexokinase and over 10 min at 24°C in the presence of human liver glucokinase. The relative extent of the inhibitory action of D-mannoheptulose (0.02-10.0 mM) was always less marked with α- than β-D-glucose. In the case of hexokinase, the experiments conducted at the high concentration of the D-glucose anomers (1.0 mM) revealed that D-mannoheptulose, at low concentrations (0.2-0.5 mM), may unexpectedly increase the phosphorylation of α-D-glucose. These findings thus document anomeric specificity in terms of the inhibitory action of D-mannoheptulose upon α- versus β-D-glucose phosphorylation by either hexokinase or glucokinase.

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October 2004
Volume 14 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Y, Courtois P, Sener A and Malaisse WJ: Dissimilar effects of D-mannoheptulose on the phosphorylation of α- versus β-D-glucose by either hexokinase or glucokinase. Int J Mol Med 14: 107-112, 2004
APA
Zhang, Y., Courtois, P., Sener, A., & Malaisse, W.J. (2004). Dissimilar effects of D-mannoheptulose on the phosphorylation of α- versus β-D-glucose by either hexokinase or glucokinase. International Journal of Molecular Medicine, 14, 107-112. https://doi.org/10.3892/ijmm.14.1.107
MLA
Zhang, Y., Courtois, P., Sener, A., Malaisse, W. J."Dissimilar effects of D-mannoheptulose on the phosphorylation of α- versus β-D-glucose by either hexokinase or glucokinase". International Journal of Molecular Medicine 14.1 (2004): 107-112.
Chicago
Zhang, Y., Courtois, P., Sener, A., Malaisse, W. J."Dissimilar effects of D-mannoheptulose on the phosphorylation of α- versus β-D-glucose by either hexokinase or glucokinase". International Journal of Molecular Medicine 14, no. 1 (2004): 107-112. https://doi.org/10.3892/ijmm.14.1.107