Upregulation of tumour endothelial marker-8 by interleukin-1β and its impact in IL-1β induced angiogenesis

  • Authors:
    • Khaled A. Rmali
    • Mahir A.A. Al-Rawi
    • Christian Parr
    • Malcolm C.A. Puntis
    • Wen G. Jiang
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.1.75
  • Pages: 75-80
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Abstract

Tumour endothelial marker-8 (TEM-8) has been found to be selectively upregulated in tumour-associated endothelial cells and is implicated in tumour specific angiogenesis. Specific factors, indigenous to tissues and tumours that regulate the TEM-8 mechanism in angiogenesis are not defined. We report for the first time that interleukin-1β induces the expression of TEM-8 in endothelial cells. Human vascular endothelial cells (HECV), which strongly express IL-1β receptor (as revealed by RT-PCR, Western blotting), increased the level of TEM-8 expression following stimulation with IL-1β (as revealed by conventional and quantitative RT-PCR). Using a newly developed antibody to human TEM-8, we have further demonstrated that IL-1β significantly raised the level of TEM-8 at the protein level, as revealed by Western blotting. In vitro tubule forming assay, revealed that IL-1β significantly induced the formation of capillary-like tubules from the HECV cells, accompanied by an increase in TEM-8 expression. It is concluded that IL-1β is a powerful regulator of the expression of TEM-8 in vascular endothelial cells. Our results suggest an important pathway through which IL-1β regulates tumour-associated angiogenesis.

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October 2004
Volume 14 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Rmali KA, Al-Rawi MA, Parr C, Puntis MC and Jiang WG: Upregulation of tumour endothelial marker-8 by interleukin-1β and its impact in IL-1β induced angiogenesis. Int J Mol Med 14: 75-80, 2004
APA
Rmali, K.A., Al-Rawi, M.A., Parr, C., Puntis, M.C., & Jiang, W.G. (2004). Upregulation of tumour endothelial marker-8 by interleukin-1β and its impact in IL-1β induced angiogenesis. International Journal of Molecular Medicine, 14, 75-80. https://doi.org/10.3892/ijmm.14.1.75
MLA
Rmali, K. A., Al-Rawi, M. A., Parr, C., Puntis, M. C., Jiang, W. G."Upregulation of tumour endothelial marker-8 by interleukin-1β and its impact in IL-1β induced angiogenesis". International Journal of Molecular Medicine 14.1 (2004): 75-80.
Chicago
Rmali, K. A., Al-Rawi, M. A., Parr, C., Puntis, M. C., Jiang, W. G."Upregulation of tumour endothelial marker-8 by interleukin-1β and its impact in IL-1β induced angiogenesis". International Journal of Molecular Medicine 14, no. 1 (2004): 75-80. https://doi.org/10.3892/ijmm.14.1.75