Effect of ghrelin on the apoptotic deletion rate of different types of cells cultured in vitro

  • Authors:
    • Anna S. Belloni
    • Carlo Macchi
    • Piera Rebuffat
    • Maria Teresa Conconi
    • Ludwik K. Malendowicz
    • Pier Paolo Parnigotto
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.165
  • Pages: 165-167
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Abstract

Evidence indicates that ghrelin, an endogenous ligand of the growth hormone secretagogue receptor, controls the growth of several human and rat cell types cultured in vitro. Hence, we have investigated, by using both TUNEL and ELISA assays, the effects of 10−8 M ghrelin on the basal apoptotic deletion rate of rat osteoblasts and thymocytes, rat and human adrenocortical cells, human umbilical vein endothelial cells, and human aldosteronoma cells cultured in vitro, as well as of the human adrenocortical carcinoma-derived cell lines NCI-H295 and SW-13. Both assays consistently showed that ghrelin did not affect apoptotic rate of normal rat and human cells, but significantly enhanced apoptotic deletion in aldosteronoma, NCI-H295 and SW-13 cell cultures. Due to the central role of apoptosis in the control of tumor growth, these findings, if confirmed in other tumor cell types, could suggest an antitumoral action of ghrelin.

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August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Belloni AS, Macchi C, Rebuffat P, Conconi MT, Malendowicz LK, Parnigotto PP and Nussdorfer GG: Effect of ghrelin on the apoptotic deletion rate of different types of cells cultured in vitro. Int J Mol Med 14: 165-167, 2004.
APA
Belloni, A.S., Macchi, C., Rebuffat, P., Conconi, M.T., Malendowicz, L.K., Parnigotto, P.P., & Nussdorfer, G.G. (2004). Effect of ghrelin on the apoptotic deletion rate of different types of cells cultured in vitro. International Journal of Molecular Medicine, 14, 165-167. https://doi.org/10.3892/ijmm.14.2.165
MLA
Belloni, A. S., Macchi, C., Rebuffat, P., Conconi, M. T., Malendowicz, L. K., Parnigotto, P. P., Nussdorfer, G. G."Effect of ghrelin on the apoptotic deletion rate of different types of cells cultured in vitro". International Journal of Molecular Medicine 14.2 (2004): 165-167.
Chicago
Belloni, A. S., Macchi, C., Rebuffat, P., Conconi, M. T., Malendowicz, L. K., Parnigotto, P. P., Nussdorfer, G. G."Effect of ghrelin on the apoptotic deletion rate of different types of cells cultured in vitro". International Journal of Molecular Medicine 14, no. 2 (2004): 165-167. https://doi.org/10.3892/ijmm.14.2.165