Differentiation of HL-60 myeloid leukemia cells induced by all-trans retinoic acid is enhanced in combination with caffeic acid

  • Authors:
    • Renata Veselská
    • Karel Zitterbart
    • Jan Auer
    • Jakub Neradil
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.305
  • Pages: 305-310
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Abstract

We investigated a possible enhancement of all-trans retinoic acid (ATRA)-induced differentiation of HL-60 human myeloid leukemia cells by caffeic acid (CA), a widely distributed plant phenolic compound. Our results showed that CA, in the concentration of 13 or 52 µM, had no or minimal influence on cell differentiation, whereas the differentiating activity of ATRA was potentiated by CA treatment. We proved, using flow cytometric detection of the CD66b surface molecule, a synergistic effect of CA: at day 10, 18.3% of CD66b-positive cells were detected after treatment with ATRA only, and 33% when CA and ATRA were combined together. NBT-assay confirmed that this additive effect of CA on ATRA-induced differentiation. Proliferating activity as assessed by MTT-assay was generally not affected by CA at given concentrations. However, cell proliferation was significantly reduced by 52 µM CA at 96-h intervals. This effect was markedly enhanced when CA, at both concentrations, and ATRA were combined. The possibility to enhance the differentiation potential of ATRA by CA may improve outcomes in the therapy of acute promyelocytic leukemia.

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August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Veselská, R., Zitterbart, K., Auer, J., & Neradil, J. (2004). Differentiation of HL-60 myeloid leukemia cells induced by all-trans retinoic acid is enhanced in combination with caffeic acid. International Journal of Molecular Medicine, 14, 305-310. https://doi.org/10.3892/ijmm.14.2.305
MLA
Veselská, R., Zitterbart, K., Auer, J., Neradil, J."Differentiation of HL-60 myeloid leukemia cells induced by all-trans retinoic acid is enhanced in combination with caffeic acid". International Journal of Molecular Medicine 14.2 (2004): 305-310.
Chicago
Veselská, R., Zitterbart, K., Auer, J., Neradil, J."Differentiation of HL-60 myeloid leukemia cells induced by all-trans retinoic acid is enhanced in combination with caffeic acid". International Journal of Molecular Medicine 14, no. 2 (2004): 305-310. https://doi.org/10.3892/ijmm.14.2.305